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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Viral supernatant was collected and concentrated after 48 hours and transduced directly into hiPSCs at low to mid-confluency along with 8 μg/mL polybrene (Fisher Scientific). Because all plasmids contained puromycin-resistance cassettes ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+/Luc+ NPCs were diluted with a final concentration of 8 × 104 cells/μL in sterile 1x PBS (pH 7.4, without calcium or magnesium; Thermo Fisher Scientific) and stored on ice until transplantation ...
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bioRxiv - Biochemistry 2022Quote: Exosomes from mice plasma were harvested from age matched WT and SR-/- mice (6-8 weeks) plasma using the total exosome precipitation reagent for plasma (Invitrogen #4484451) as per instructions ...
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bioRxiv - Biochemistry 2022Quote: ... using a 10-minute 8-38% acetonitrile gradient (200 ml/min) and subsequent MS2 by HCD on a Fusion Lumos mass spectrometer (ThermoFisher Scientific). Phosphorylated residues (serine ...
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bioRxiv - Neuroscience 2022Quote: ... then the flow-throughs were fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... and 8×104 cells were plated into 96-well U-bottom and activated with anti-CD3/anti-CD28-coated Dynabeads (Thermo Fisher), as previously described89 ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 with daily replacement of Essential 8 media system (Thermofisher, Canada. Catalog#A1517001). Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 µM Z-IETD-R110; Molecular Probes), caspase-9 (10 µM ZLEHD-R110 ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μg of anti-rabbit IgG was incubated with 8 μg of Alexa Fluor 750 N-Succinimidyl Ester (Thermo Fisher Scientific) in 100 mM NaHCO3 (pH 8.3 ...
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bioRxiv - Microbiology 2021Quote: ... One microliter of G3 phages were denatured at 94°C for 8 min and used as a template for PCR using Phusion High-Fidelity PCR Master Mix (Thermo Fisher). The amplified DNA fragment was purified using QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... emerging clones were manually picked and cultured under feeder-free conditions using Geltrex-coated dishes and Essential 8 medium (Life Technologies). iPSC clones were maintained in culture for at least 15 passages to ensure stable pluripotency before characterization was conducted by the iPSC – cell reprogramming core facility of CHU Sainte-Justine ...
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bioRxiv - Biochemistry 2020Quote: ... 1*10^8 yeast were pelleted and resuspended in 500 µl PBM containing 100 nM Streptavidin-Alexa Fluor 647 conjugate (Thermo Fisher) and 400 nM PLpro S1*-mutant and incubated for 40 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were measured by adding 2uL of stock solution (100 nM) to an 8 uL droplet of filtered phosphate buffered saline (Gibco 14190144). Contrast measurements were converted to molecular weights using a standard curve generated with bovine serum albumin (Thermo 23210 ...
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bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies ...
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bioRxiv - Genetics 2022Quote: ... or one of five Hsa21 genes (100 ng/well; 1 row, 8 wells or technical replicates per unique cDNA) using Lipofectamine 2000 (Invitrogen, 11668030) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The iPSCs were continuously cultured and expanded on Geltrex coated 6-well plates with Essential 8 medium (ThermoFisher Scientific, Cat#A1517001). Human iPSC cell lines used in this manuscript ...
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bioRxiv - Systems Biology 2022Quote: ... reconstituted in aqueous 0.1% TFA and separated (according to the instructions supplied by the manufacturer) into 8 fractions using the Pierce high pH reversed-phase fractionation kit (Thermo Scientific). For plants 2 (DX ...
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bioRxiv - Neuroscience 2022Quote: ... 1xPBS was replaced with direct stochastic optical reconstruction microscopy (dSTORM) photo-switching buffer consisting of 50 mM Tris pH 8 solution supplemented with 10 mM sodium chloride (ThermoFisher Scientific), 10% glucose (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Immunology 2022Quote: ... The 8-10 μm tissue sections were cut with cryostat (NX70 MicromMicrotech) and we used the Tyramide SuperBoost™ kit (Invitrogen) according to the manufacturer’s instructions for immunofluorescence staining ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Neuroscience 2022Quote: The tissue slice was rehydrated in PBS at room temperature (RT) (2 × 15 min) and incubated in PBS with 8% SDS (Fisher Scientific) at RT (4 h ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg of DNA was used to infect 8 X 105 SF9 cells by using Cellfectin II (Thermo Fisher Scientific; 10362100) two times to increase viral titer to 5 X 106 plaque-forming units/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 μM Z-IETD-R110; Molecular Probes), caspase-9 (10 μM Z-LEHD-R110 ...
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bioRxiv - Biophysics 2019Quote: ... For live cell microscopic analysis, stable DM cells were plated in tomo-dishes (Tomcube, Korea) or Lab-Tek 8-well chambered coverglass (Nunc, USA). For observation of HeLa cells expressing H2B-mRFP ...
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bioRxiv - Cell Biology 2019Quote: ... Emerging hiPSC colonies were manually picked and cultured under feeder-free conditions in Essential 8 medium on Geltrex-coated dishes (Life Technologies). hiPSC clones were passaged at least 15 times to increase stable pluripotency ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
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bioRxiv - Neuroscience 2019Quote: ... seeded at 1.8 × 105 cells/cm2 in plastic plates coated with poly-D-lysine (10 mg/ml) and incubated in Neurobasal (Life Technologies) supplemented with 2 mM glutamine ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Biophysics 2020Quote: The tau cysteine mutants were buffer exchanged into 20 mM NaPi buffer (pH 8) via 7k MWCO Zeba spin desalting columns (Thermo Fisher). 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Suspended cells were sonicated for 8 cycles (30s on 30s off) with 30% amplitude on ice using probe sonicator (Sonics vibra cells, Fisher scientific) followed by centrifugation at 9000 rpm for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... Neuro-2a cells were seeded into the 8-well chambered coverglass (Nunc™ Lab-Tek™ Chambered Coverglass, Thermo Fisher Scientific) at the density of 15,000 cells/well ...
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... mixed at an 1:1 ratio in a test tube and added to wells of an 8-well slide (Thermo Fisher) coated with poly-lysine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Cancer Biology 2020Quote: After 48 hours incubation in the presence of the compounds, cells were fixed by the addition of an equal volume of formaldehyde (8 %, 50 µL) (#28908, Thermo Scientific) to the existing media ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... ermGal4) or their unsorted control matches were plated on cover glass (Labtek II Chambered Coverglass, 8-well, 155409, Thermo Fisher Scientific) into Schneider’s medium (Homem ...
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bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then denatured at 95°C for 8 min in the presence of 4X LDS sample loading buffer (Life Technologies) and 5% beta-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Dried fractions were resuspended in 8 μL 5% ACN + 5% FA and analyzed on an Orbitrap Fusion with in-line Easy Nano-LC 1000 (Thermo Scientific). Fractions were run as 3 h gradients progressing from 3% ACN + 0.125% FA to 100% ACN + 0.125% FA ...