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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cartilage fragments were subsequently cut with an 8 mm diameter disposable biopsy punch (Robbins Instruments) and cultured for 24h in DMEM/F12 (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: 1.0×104 of PDAC cells were seeded on 8-well NuncTM Lab-TekTM II CC2TM Chamber Slides (Thermo Fisher Scientific). Cells were washed with PBS and fixed in 4% paraformaldehyde in PBS at room temperature for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168; PC Bottle Assembly 70 mL, ThermoFisher) for 1 hour at 4° C ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Cdc13-encoding baculovirus samples were generated by transfecting of 1 µg of bacmid DNA in 8 µL Cellfectin II reagent (Gibco) into 8×10 Sf9 cells in one well of a 6-well plate ...
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bioRxiv - Microbiology 2024Quote: ... at 120 rpm with 8% CO2 following the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were resuspended in 45 μL of 1X Ligase buffer containing 8 nM of adapter and 2.5 units of T4 DNA ligase (ThermoFisher, 15224041) and incubated at room temperature for 60 min ...
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bioRxiv - Cell Biology 2024Quote: Mixed populations of keratinocytes were prepared and seeded onto 8-chamber glass slides with removable wells (Lab-Tek, ThermoFisher #154534). Cells were grown to approximately 50% confluence ...
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bioRxiv - Bioengineering 2024Quote: ... Wells were loaded at 400 ng (standards) or 100 µg total protein/well into a Novex WedgeWell 8% Bis-Tris gel (Invitrogen) and separated in Tris-glycine SDS running buffer (Novex) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...
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bioRxiv - Bioengineering 2024Quote: ... 200 μl of antibody or scFv at 1 mg/ml in PBS was mixed with 10% volume of 1M sodium bicarbonate to adjust pH to 7-8 followed by addition of EZ-Link sulfo-NHS-LC-Biotin (ThermoFisher) to reach a 5:1 biotin:protein molar ratio ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellets were resuspended in 500 μl of 8 mg/ml dithiobis (succinimidyl propionate; DSP) solution (Thermo Fisher Scientific) and cross-linked ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were centrifuged from the culture medium and resuspended in an equal amount of Fluo-8 dye-loading solution along with Hank Balanced Salt Solution (HBSS buffer) supplemented with calcium (Gibco). A suspension containing 200,000–250,000 P2X3-Expi293 GnTI- cells per well was prepared in a 96-well poly-D-lysine plate ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: HEK293T/17 (ATCC #ACS-4500) and HCT-8 (ATCC #CCL-244) cells were cultured in Dulbecco’s modified Eagle medium (DMEM; ThermoFisher #11995065) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) and 8 µg/mL of blasticidin (all Gibco brand, from Thermo Fisher Scientific). The SH-SY5Y cell line was cultured in the minimum essential medium Eagle (EMEM ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) and 8 µg/mL of blasticidin (all Gibco brand, from Thermo Fisher Scientific). The SH-SY5Y cell line was cultured in the minimum essential medium Eagle (EMEM ...
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bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 cells carrying an inducible copy of GFP multimers and FlpIn T-Rex 293 cells carrying an inducible copy of 3×FLAG-SNAP-SAF-A were grown in 8-well Lab-Tek II Chambered Coverglass -- 1.5 borosilicate glass (Thermofisher scientific).
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bioRxiv - Biochemistry 2024Quote: ... followed by an enzyme solution consisting of 8 nM wild-type PETase or its mutant labeled with 0.8 nM Qdot655 (Thermo Scientific), 2 mM 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... alongside control human embryonic stem cell (CTRL-ES) and FMR1 knock out line (FMR1KO) used in this study were maintained in Essential 8 Medium (E8, Gibco) in plates coated with Matrigel Growth Factor Reduced Basement Membrane Matrix and were passaged at 80% confluence with 1 mg/ml collagenase and 0.5 mg/ml dispase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of cRNA was used for reverse transcription with 2 pmoles of a 16S rRNA-specific primer (Supplementary Table 8) and SuperScript II (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
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bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... After neutralization with 1/10 volume of 1 M sodium phosphate buffer (pH 8) the antibody was coupled to M270 Epoxy Dynabeads (Thermo Scientific) in ammonium sulfate buffer (0.1 M sodium phosphate pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cancer Biology 2021Quote: Cells stably expressing V5-tagged XPR1 and KIDINS constructs were plated at a density of 10,000 -20,000 cells per well in NuncTM Lab-Tek™ II CC2™ 8-well Chamber Slides (Thermo Fisher). When investigating localization upon knockdown ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from 8/16-cell embryos (10-15/group/replicate) was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Purified RNA was then subject to reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Genetics 2020Quote: ... embryos were washed 8 × 15min in PBST (PBS with 0.1% Tween-20) and incubated in Image-iT FX Signal Enhancer (ThermoFisher Scientific, I36933) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific, C10337). For induction of apoptosis in myoblasts ...
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bioRxiv - Microbiology 2021Quote: ... was seeded with influent solids harvested from the influent of the RAEBL full-scale WRRF by weekly centrifuging 10 L of a 24-h composite influent sample at 4,000×g using the IEC-Centra-8 centrifuge (Thermo Scientific, USA). The total suspended solids (TSS ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Synthetic Biology 2022Quote: HEK:BFP cells were the kind gift of Chris Richardson.8 Cells were maintained at 37°C and 5% CO2 in DMEM media plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 7 days and 14 days post SNI) L3 and L4 DRGs were dissected from 2 mice (8 DRGs/sample) and stored in ice-cold HBSS (Gibco, 14170088). DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were centrifuged again (80 g, 8 min) and suspended at the desired concentrations in culture medium (Neurobasal medium (Thermofisher 21103049) supplemented with 1 % GlutaMAX (Thermofisher 35050) ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2020Quote: Flash frozen MCAs with surrounding brain tissue were sliced to 8 μm sections using a cryotome (Fisher Scientific, Pittsburgh, PA, USA), placed on charged slides (2-4 slices/slide) ...
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bioRxiv - Molecular Biology 2020Quote: INS1 cells were re-plated onto a cover slip as above and incubated in regular INS1 medium containing 8 μM Bodipy C12 (Molecular Probes) at 37 °C at 5% CO2 for the last 16 h of culture ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with indicated plasmids alone or in combination for 8 h by using Lipofectamine 3000 (Thermo Fisher, USA), and then continue to cultivate for 24 h in the fresh medium before being subjected to indicated experiments.
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Each CDM pellet was resuspended in 100 mM NH4HCO3 solution containing 8 M urea and 10 mM dithiothreitol (DTT; Thermo Fisher) and incubated at 37 °C for 2 hours under agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 25 µL drops of cell-Matrigel suspensions were seeded into each well of an 8-well chambered coverglass (Nunc, No. 1.5) and incubated at 37 °C for 30 minutes before adding DMEM to cells ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... HEK293T cells in a 10 cm dish with about 60% confluence were transfected with 8 μg lentiviral construct and helper plasmids using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... for NIH-3T3 cells and P19 cells or 5nM siRNA/8 µl INTERFERin (PolyPlus) for MDA-MB-231s and 200 µl Opti-MEM (ThermoFisher Scientific) in fresh media ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA produced from 15 ng RNA and 8 pmol of each forward and reverse primer per reaction and Power SYBR Green PCR Master Mix (Applied Biosystems) were used ...