Labshake search
Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
-
bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
-
bioRxiv - Systems Biology 2019Quote: ... Human ESCs (WA-01; National Institutes of Health registry #0043) were maintained in Essential 8 medium (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin-amphotericin B solution (Lonza ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The homogenate was centrifuged at 10,000g for 15 minutes (Sorvall™ ST 8 Small Benchtop Centrifuge: Thermo Scientific, Waltham, MA, USA). The supernatant was used as the enzyme source ...
-
bioRxiv - Molecular Biology 2019Quote: Human atherosclerotic plaque or mouse aortic root specimens were first cut into 8 μm sections using a freezing microtome (Thermo Scientific). For immunofluorescence detection to detect Tf ...
-
bioRxiv - Biophysics 2019Quote: ... were plasma-cleaned for 10 minutes in an Harrick Plasma Cleaner (Harrick) and attached to 8-well LabTek chambers (Nunc, USA), for which the bottom glass slide had been removed ...
-
bioRxiv - Genetics 2019Quote: Xenograft mouse model of human lung cancer tumors were implanted under the left upper limb in the abdomens of 6- to 8-week old male NCG mice by subcutaneous injection of A549 (5×106 cells in 200 µL DPBS (Gibco, C14190500BT)) or H2228 cells (2× 106 cells in 200 µL DPBS) ...
-
bioRxiv - Plant Biology 2020Quote: ... Blocks were sectioned using a rotary microtome to 8 μm thickness and then floated on Arcturus PEN membrane slides (Fisher Scientific) with DEPC-treated water ...
-
bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... Confluent cells were first differentiated overnight with serum-free DMEM in 8-well chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific). Cells were then washed and treated with anesthetics or other drugs for 10 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS ...
-
bioRxiv - Microbiology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
-
bioRxiv - Neuroscience 2020Quote: ... 8 µg of the cDNA from each sample were fragmented and labelled with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753). Hybridization was performed as described previously31 during 16 h at 45°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Embryos older than 24 hpf were permeabilized in 10 μg/mL Proteinase K solution (8 min for 24 hpf, 28 min for 48 hpf, 30 min for 5dpf and 7dpf embryos) (Invitrogen, 25530015). ISH was then performed as described previously (Thisse and Thisse ...
-
bioRxiv - Developmental Biology 2021Quote: ... Coriell) were maintained under feeder-free conditions using Essential 8 (E8) Medium and vitronectin (VN)-coated plates (#A14700; Thermo Fisher Scientific). Cells were routinely passaged using 0.5 mM EDTA diluted in phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... with a mu- phosphatase signal sequence and a C-terminal Avi-8xHis-EPEA-tag in a pD2610-V5 vector (ATUM Bio) were expressed in Expi293F cells at 37°C and 8% CO2 according to manufacturer’s instructions (Thermo Fisher Scientific). Cell culture supernatant was collected after four days and purified over a 5 mL C-tag affinity matrix (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: Human induced pluripotent stem cells (hiPSCs) were maintained in feeder-free monolayers on Geltrex (ThermoFisherScientific) and fed daily with Essential 8 medium (Life Technologies). When confluent ...
-
bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
-
bioRxiv - Bioengineering 2020Quote: FACS-purified CD3-CAR+ or CD3-CAR- CD4+ T cells (8 x 106 each) were lysed in Pierce™ IP Lysis Buffer (ThermoFisher) supplemented with Complete Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Bioengineering 2020Quote: iPSCs (DF19-19-9-11T) purchased from WiCell Technologies were maintained on vitronectin-coated plates in Essential 8 medium (E8, ThermoFisher Scientific), and passaged when the colonies reached ~75-80% confluence ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 % of the input and all of the output volume were loaded on an SDS-PAGE using 8-12 % Novex Wedge Well Tris-Glycine gel (Thermo Scientific). The gel was then imaged on Amersham Typhoon (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... All iPSC lines were maintained on Vitronectin-coated plates and fed with Essential 8 (E8) + E8 supplement media (ThermoFisher, CAT#: A1517001).
-
bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
-
bioRxiv - Neuroscience 2021Quote: ... Coverslips were mounted on glass microscope slides using 8-10 µl of Prolong Gold Antifade mounting media with DAPI (Invitrogen, P36941) per coverslip ...
-
bioRxiv - Neuroscience 2021Quote: ... and ESC (WiCell H9 WA09, H7 WA07) lines were maintained on feeder free conditions on Essential 8 media (E8, Life technologies) and Geltrex (Life technologies ...
-
bioRxiv - Immunology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
-
bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
-
bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were stained with primary antibodies: cytokeratin-8 (1:100; TROMA-I, DSHB) and cytokeratin- 14 (1:250; Invitrogen, PA5-16722) and with species-specific fluorochrome-conjugated secondary antibodies (1:500 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Bead-binding was performed using 30 pl of Protein G Dynabeads (Thermo Fisher Scientific Baltics UAB, V.A. Graiciuno 8, LT-02241 Vilnius, Lithuania. Thermo Fisher Scientific Baltics UAB complies with Quality System Standards ISO 9001 and ISO ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells from both hippocampi of each mouse were pooled together and plated in 2 wells of an 8-well Chambered Coverglass (ThermoFisher, 155409PK) at a density of ~250,000 cells per chamber ...
-
bioRxiv - Neuroscience 2020Quote: ... with titan rotor T29-8×50 in oak ridge centrifuge tubes with sealing caps (all from Thermo Fisher Scientific, Rochester, NY). After removal of the supernatants ...
-
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... For analysis of mitochondrial fragmentation cells were plated at 20,000 per well in NuncTM Lab-TekTM II Chambered Coverglass (8 well, 155409, Thermo Fisher Scientific).
-
bioRxiv - Physiology 2020Quote: ... Upon achieving confluence, they were treated with 8 μM GSK3 inhibitor (CHIR99021, Stemgent) in RPMI supplemented with B27 lacking insulin (Thermo Fisher). 24 hrs later ...
-
bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2020Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hr ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... Plates were blocked on the following day for 1hr with TBS-TMS (20mM Tris pH 8, 150mM NaCl, 0.05% Tween 20, 1% non-fat dry milk, 1% goat serum (Gibco 16210-072)) ...
-
bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then fractionated into 8 fractions using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific 84868), then dried by speed-vac ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were DNAseI treated and cDNA was generated from 8 μL of purified RNA/individual (∼500 ng of RNA) using SuperScript™ III First-Strand Synthesis System (ThermoFisher). cDNA was diluted 2-fold with RNase-free water then expression levels of selected immune genes was determined by amplifying 1 μL of cDNA with primers for cecropin D (AAEL000598 ...
-
bioRxiv - Systems Biology 2020Quote: ... the differentiated cardiomyocytes were re-plated in Nunc™Lab-Tek™II glass-bottomed 8-chamber glass slides (Thermo Fisher), and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Reactions were assembled in either 8-tube Genie strips (OP-00008, OptiGene Ltd) or 96-MicroAmp-Fast-Optical reaction plate (Applied Biosystems). Strip tubes reactions were capped ...
-
bioRxiv - Microbiology 2020Quote: ... EBV producer HEK-293 cells stably transformed by BART-repaired B95-8 based EBV BAC system encoding GFP (45) were cultured in RPMI 1640 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
-
bioRxiv - Cancer Biology 2020Quote: ... GFP antibody and HER2/neu Ab-8 (Clone e2-4001) mouse monoclonal antibody used for IP were obtained from Thermo Fisher. C6orf97 antibody is from GeneTex ...
-
bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
-
bioRxiv - Genomics 2021Quote: ... and 0,8 mg/ml BME V2 were added in a volume of 50µl per well using a Multidrop dispenser (Thermo Fisher Scientific). Plates were sealed with a plate-loc (Agilent ...