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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... DNA samples were eluted in 30 µl 10 mM Tris HCl pH 8 and analysed for purity and quantity using the Nanodrop spectrophotometer (NanoDrop One, ThermoFisher Scientific) and Qubit fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... washed in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA, USA) two times ...
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bioRxiv - Microbiology 2021Quote: ... and 10 ml of 1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Life Technologies). Sf9 insect cells (ATCC CRL-1711 ...
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bioRxiv - Microbiology 2022Quote: ... for nucleic acids (bacteria and host) and 4 µM ER-Tracker Red (Thermo Fisher Scientific) for ER in Buffer A for 2 h at 37°C on the SkyLight Glass Base Dish 3971-035-SK (IWAKI ...
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bioRxiv - Bioengineering 2022Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 1 M was purchased from Gibco (Waltham, MA), sodium chloride 5 M was purchased from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1% formic acid in acetonitrile (B) (A998-4, Thermo Fisher Scientific, Waltham, MA, USA). The column temperature was set to 45 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2% formic acid and injected 4 μL into an Easy-nLC 1200 (Thermo Fisher Scientific) HPLC system ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were hydrolyzed into their protein-bound amino acids in a sealed glass tube at 150 °C for 2 h with 500 μL methanesulfonic acid 4 M (Fisher Scientific; Hampton, New Hampshire, USA) after flushing out air with a gentle stream of nitrogen gas ...
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bioRxiv - Biophysics 2021Quote: ... were plasma cleaned for 10 min and attached with the use of dental imprint silicon putty (Picodent twinsil 22, Picodent) to Lab-Tek™ 8-well chambers (ThermoFisher Scientific), from which the glass bottom had been removed34 ...
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bioRxiv - Cancer Biology 2021Quote: MEFs were plated in 8-well cover glass bottom chambers (Lab-tek #155409) and loaded with cSNARF-1-AM [10 μM] (Thermo Fisher #C1272), a fluorescent pH-sensitive dye ...
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bioRxiv - Cancer Biology 2021Quote: ... each coverslip was inverted onto a 30- μl droplet of 1:8 Oregon Green™ 488 Conjugate Gelatin (Life Technologies, Cat. #G13186) with 0.1% porcine gelatin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... tissue culture flasks or MATTEK glass bottom dishes were incubated in sufficient volume of a mixture of Essential 8 (E8) media (ThermoFisher cat. # A1517001) and Matrigel (Corning cat ...
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bioRxiv - Molecular Biology 2021Quote: ... 5-8 × 106 autogamous cells (T=50 hours) were collected by centrifugation and cross-linked with 1% methanol-free formaldehyde (Thermo Fisher Scientific) PHEM 1 × (10 mM EGTA ...
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bioRxiv - Neuroscience 2021Quote: ... a transcription factor neurogenin-2 in a doxycycline inducible cassette was stably integrated into the AAVS1 safe harbour locus of iPSCs cultured in Essential 8 media (ThermoFisher Scientific, A1517001). The expression of neurogenin-2 in the iPSCs was induced for three days using doxycycline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... about 5×104 of transfected cells were digested and placed into the upper chamber precoated 8 μm pore transwell insert (Fisher Scientific, 0877121) with the lower chamber containing the medium (containing 10% FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... frozen WTC11 cells were first thawed in 37 °C water bath and washed in Essential 8 Medium (E8; Thermo Fisher Scientific, #A1517001) by centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... The bottom layer of the culture was first prepared by pouring 100 μL of type I collagen mix solution into each well of an 8-well coverglass chamber (177402PK, Nunc, Roskilde, Denmark). The middle layer of the culture was prepared by adding 100 μL of the type I collagen mix solution containing 3 × 103 cells/well of the Klf5-iKO line and laid over the bottom layer ...
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bioRxiv - Immunology 2021Quote: ... The peptides from the Hemocyte samples collected at four different time points were labelled with four 8-plex iTRAQ reagents (Applied Biosystems, USA).
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bioRxiv - Pathology 2019Quote: ... with 8 mM EDTA for 10 min at 75 °C and then converted to cDNA using Superscript IV reverse transcriptase (Invitrogen, Stockholm, Sweden) in a 20 μl reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were then washed with TBS-T buffer followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1h and visualized on either Kodak X-ray film (GeneMate, F-9023-8×10) or BioRad ChemiDoc Imaging System using either SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, 34080) or ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... RPE-choroid explants were washed with phosphate-buffered saline (PBS) and 300 μl of 8 µg/ml Calcein AM (Thermo Fisher Scientific) applied to each explant and incubated for 1 h at 37°C (5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: Passaging in competition assays was performed by co-transfecting BHK-21 cells with in vitro transcribed replicon RNA and harvesting total cell RNA at 8 hours post transfection using TRIzol reagent (Thermo Fisher Scientific). The harvested RNA was then purified using the Direct-zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The spheroids were cultured for 8 days and pictures were taken with an AMG Evos microscope 40x objective (Thermo Fisher Scientific Inc). Spheroid areas were measured using ImageJ 1.51j8 software (National Institute of Health ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from the H661 cells after DMSO or ENT treatment (duplicate samples for each treatment) for 8 hours using TRIzol (Invitrogen, Carlsbad, USA) and a miRNeasy mini kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... Following tissue digestion 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA) was added to stop enzyme activity ...
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bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
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bioRxiv - Biophysics 2020Quote: ... were plasma cleaned for 10 min and attached with the use of dental imprint silicon putty (Picodent twinsil 22, Picodent) to Lab-Tek™ 8-well chambers (ThermoFisher Scientific), from which the glass bottom had been removed42 ...
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bioRxiv - Biochemistry 2020Quote: ... 6 to 8-week old male mice (C57BL/6J) were injected via tail vein with 29 G syringe (ThermoFisher 14-841-32) at a dose of 10e11 GC/mouse diluted in saline in a total volume of 100 μl/mouse ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated by papain digestion and plated on poly-D-lysine-coated (0.01 mg/mL) 8-well Lab-Tek II chambered cover glass (Thermo Fisher Scientific, #Z734853). Specifically ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg protein was loaded per well on 8% acrylamide gels with prestained standard molecular weight markers (PageRuler prestained protein ladder; Thermo Fisher Scientific) and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Overexpressed myc-flag-tagged cadherin-8 was immunoprecipitated using the Pierce c-My-tag IP/Co-IP kit according to the manufacturer’s protocol (Thermo Fisher Cat#23620). Co-immunoprecipitated proteins were determined by Western blot analysis.
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... 20 μl of untreated and of ProK treated PMV (before dilution for FRET) was loaded to 8 % SDS-PAGE gels and the proteins were stained with GelCold Blue Stain (Thermo Fisher Scientific). A control in which PMSF was added for 15 min at room temperature before adding ProK also prepared and loaded on the gel.
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Microbiology 2021Quote: ... and sacculi were spun down at 22,000 x g at 40 °C for 30 min in a Heraeus Multifuge X1R using a Fiberlite™ F15-8 x 50cy Fixed Angle Rotor from Thermo Scientific. SDS was removed by washing the sacculi with 60 °C pre-warmed Millipore water several times until no SDS was detected by the methylene blue assay [Hayashi ...
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bioRxiv - Neuroscience 2020Quote: ... The middle segment was used to cut 8 μm serial sections using rotary microtomes (HM 200 and HM 355; Thermo Scientific, Germany) which were mounted on glass slides ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 8% of fetal bovine serum (FBS) and 1% of penicillin-streptomycin (PES 10, 000 u/mL, Thermo Fisher, NY, USA). Macrophages were seeded over sterile coverslips in a ratio of 250 × 103 / cm2 and they were maintained at 5% CO2 atmosphere at 37 °.
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bioRxiv - Microbiology 2020Quote: ... U-2 OS cells were cultured to 80% confluency in Nunc™ 8-well Lab-Tek II Chambered Coverglass slides (Thermo Scientific). Upon infection ...
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bioRxiv - Microbiology 2020Quote: ... EBV producer HEK-293 cells stably transformed by BART-repaired B95-8 based EBV BAC system encoding GFP (45) were cultured in RPMI 1640 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Microbiology 2020Quote: ... Cover slips were then washed three times for 5 min in wash buffer and subsequently mounted on slides with 8 μl of prolong glass (Invitrogen, cat. # P36984) or mowiol ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h, CCK-8 (Dojindo, Japan) was used to measure drug sensitivity at 450 nm using a microplate reader (Thermo Fisher, USA) after incubating at 37 °C for 1–2 h.