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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Immunoprecipitations for competition assays were performed with 8 μg of C-terminally biotinylated nanobody on M-280 streptavidin magnetic beads (Dynabeads, Invitrogen) and 10 μg of spike or RBD preincubated with 10 μg of the indicated (HIS-tagged ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... containing 50 mM dithiothreitol (DTT) for 5 min at 100°C before loading on 8-16% Tris-glycine gels (ThermoFisher). Gels were electrophoresed at RT in running buffer (25 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... The tubes were then supplemented with 630 μl chloroform/isoamyl alcohol (24:1, pH 8, Acros Organics, Cat. No.: 327155000) and bead beat at setting 6 for 40 sec using the FastPrep-24™ Classic Instrument (MP Biomedicals ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... 72 µg PE-Puro or PE-FeLV plasmid were mixed with 8 µg pCS2-GFP and 40 µl Lipofectamine P3000 (Invitrogen) in 3.2 ml Opti-Mem (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... The mixed cell suspension was dispensed into a large dimple plate (Tissue by Net Ltd., Tokyo, Japan) for mixed spheroids formation in Essential 8 Medium (A1517001 Invitrogen) for four days before being collected and transferred to a 3D printed mold ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: Murine MC38-RFP adenocarcinoma cells and MC38 hybrid cells (MC38-macrophage hybrid cells)8 were cultured in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and embedded in Tissue-Tek O.C.T Compound (Sakura) and cut into 8 μm sections using CryoStar NX50 (Thermo fisher Scientific). Slides were permeabilized with 0.3% Triton X-100 (Beyotime ...
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bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... cells were plated in three biological replicates in an 8-well Lab-Tek II chambered cover-glass system plate (Nunc) or 24-well plates at a density of 100,000 cells/well in 500 µL of media and incubated at 37 °C under a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Jupiler with increased ethanol levels (8% and 13.5% ABV) (adjusted using 100% ethanol, Fisher Chemical, Thermo Fisher Scientific, Waltham, USA); dry-hopped Jupiler (using Cascade Cryo Hops® pellets ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α) were measured using a custom 8-plex panel mouse ProcartaPlex assay (ThermoFisher Scientific, catalog# PPX-08-MXGZGFX), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... for a total of three replicates per condition and fragmented for 8 minutes at 70°C to an average fragment size of ~80 nt using Fragmentation Reagents (Invitrogen). rRNA depletion ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Microbiology 2022Quote: ... To prepare CONV larvae first instars from surface-sterilized eggs were exposed immediately upon hatching to CONV inoculum at selected dilution (from 10−3 to 10−8) in sterile water (Gibco). Bacterial composition of CONV inoculum is provided (FIG S2 ...
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bioRxiv - Molecular Biology 2022Quote: Live cell imaging of fluorescent cells was carried out in Nunc Lab-Tek II 8 well chambered coverglass in Live Cell Imaging Solution (Invitrogen) using a 40x EC PlnN oil objective with NA=1.3 on a Zeiss AxioObserver Z1 with an AxioCam MRm monochrome digital camera ...
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bioRxiv - Molecular Biology 2023Quote: Proliferating C2C12 myoblasts were transduced with lentivirus in the presence of 8 µg/ml polybrene and selected with 2 µg/ml puromycin (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Cells were mounted on microscopy slides using GLOX anti-fade buffer containing 10 mM Tris pH 8 (Thermo Fisher, AM9856), 2x SSC (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100 μg of protein in 10-40 μl volume was electrophoresed through 8% or 12% Bolt Bis-Tris Plus gels (InVitrogen) in Bolt MOPS or MES SDS running buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded as single cells at a concentration of 200K cells/cm2 in maintenance medium (Essential 8 medium, Thermo Fisher) supplemented with a rho kinase inhibitor (Stemgent ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Neuroscience 2022Quote: ... in EER mode with a flux of 8 electrons/pixel/s and a Selectris-X energy filter (Thermo Fisher Scientific) with a slit width of 10 eV to remove inelastically scattered electrons ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Perfusion was subsequently changed to bicarbonate-buffered extracellular solution supplemented with the voltage- sensitive dye Di-8-ANEPPS at 3 µM (D3167, Invitrogen). Following dye loading for 4 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG) – a proxy for oxidative DNA damage – was analyzed in corals using DNA Damage Competitive ELISA Kit (Invitrogen) and MyCurveFit.com software as described in (20).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell culture supernatants were collected and analyzed for IL-8 release using a commercially available ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and submerged in cutting buffer (8 mM Na2HPO4.2H2O, 2 mM KH2PO4, pH = 7.4) in the Micron HV650 (Thermo Fisher Scientific). Coronal sections of 60µm were cut using a frequency of 70Hz ...
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bioRxiv - Neuroscience 2024Quote: ... stock solution (8 mg/mL) was freshly prepared by dissolving RB powder in sterile saline (catalog number: NC9054335, Fisher Scientific) on the day of photothrombosis ...
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bioRxiv - Bioengineering 2024Quote: ... the medium of the electroporated U2OS cells on the 8-well microscopy slides was exchanged with 300 µl of fresh medium containing 500 ng/ml Hoechst33342 (H3570; Invitrogen) and no phenol red ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher) and loaded onto a MonoQ 5/50 GL column for anion exchange chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher), then concentrated to ∼0.5 mL and loaded onto a Superose 6 Increase 10/300 column pre-equilibrated in GF150 buffer ...
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bioRxiv - Genetics 2023Quote: ... Testes of 8 weeks old mice were ground under liquid nitrogen and RNA was extracted using Trizol (Thermo Fisher Scientific) according to the manufacturer’s instructions but using 80% EtOH during the wash step.
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bioRxiv - Cell Biology 2023Quote: ... The iPSCs were cultured in Essential 8 (E8) medium containing E8 supplement and 100 μg/mL Penicillin-Streptomycin (E8 complete medium, all from GIBCO, Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: Monolayers and EBs from day 3 to day 8 were dissociated to single cells with TypLE 1X (Thermo Fisher, 12563029) for 5 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 1M cells were plated in 10cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... Cells were grown for six days at 37°C with 8% CO2 in shaking conditions at 125 rpm according to the manufacturer’s protocol (Thermo Fisher). ExpiFectamine™ CHO Enhancer and ExpiCHO™ Feed were added 18 to 22 hours post-transfection to boost transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection was performed with a total of 12 μg of library and 8 μg of VSV-G using Lipofectamine 2000 (Invitrogen) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The uptake reaction was stopped after 8 min by washing the cells thrice with ice-cold phosphate-buffered saline (PBS, Gibco). Lysis buffer containing 1 M NaOH and 2% Triton X-100 in H2O was added ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 8 μm paraffin sections were prepared using a Leica rotary microtome RM2265 and transferred to polysine coated slides (ThermoFisher Scientific), dewaxed ...
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bioRxiv - Neuroscience 2023Quote: ... the hippocampus form 6-8 neonatal mice was collected from both hemispheres in Hank’s buffered saline solution (HBSS, Life Technologies). Tissue was incubated in 0.25% trypsin (Life Technologies ...