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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... malayi adult female tissue (see section “Parasite material and treatment”) was cryosectioned longitudinally to 8 µm thickness with a CryoStar NX70 cryostat (ThermoFisher). All sections (∼11-16 sections per block ...
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bioRxiv - Immunology 2023Quote: ... were distributed into four wells of a 24-well plate and activated for 8 days by adding prewashed anti-CD3/CD28 Dynabeads at a 1:1 bead-to-cell ratio (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Genomics 2022Quote: ... and resuspended in 1x lysis buffer (10mM Tris pH 8, 5mM EDTA, 0.5% SDS, 1x (0.4mg/ml) Proteinase K (Thermo Fisher Scientific) in water at 10M cells/800ul ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: DRG were dissected from 8- to 10-wk-old C57BL/6J mice in cold Hank’s balanced salt solution (HBSS) (Life Technologies) or Dulbecco’s Modified Eagle’s medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... were then seeded at a density of ~8 × 104 cells/cm2 in EpiLife medium containing 60 µmol/L CaCl2 (#MEPI500CA Gibco) and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Microbiology 2023Quote: ... Samples with an RNA Integrity Number (RIN) of 8 or more were used for rRNA depletion using a Ribominus v2 kit (ThermoFisher) and both bacteria-specific and human-specific rRNA probes as per the manufacturer’s instructions (Life Technologies) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... SARK and HMW tau extracts (8 ng total tau per well) were incubated with 1% lipofectamine 2000 transfection reagent (ThermoFisher) in opti-MEM (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cortex tissue from up to 8 embryos was pooled into 1.5 mL microfuge tubes with Hank’s balanced salt solution (HBSS) (Gibco -14170-112), rinsed twice with fresh HBSS ...
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bioRxiv - Biophysics 2023Quote: ... samples were mixed with 2X Novex Tris-glycine native sample buffer and loaded into NuPAGE 3-8% Tris-glycine gels (Invitrogen) with NativeMark unstained protein standard (Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... We section organoid blocks at 8 µm and mount them on Superfrost Plus Microscope Slides (12-550-15, Fisher Scientific). Hematoxylin and eosin staining was performed on the parent tumor and the resulting organoids derived from tissue collection according to standard protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Genomics 2023Quote: ... medium containing 2 µg/ml puromycin and 8 µg/ml polybrene (HCT116-Cas9) and 1 µg/ml (iPSC-iCas9) or no (NPCs- iCas9) puromycin (GIBCO) was exchanged ...
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bioRxiv - Microbiology 2023Quote: ... Fractions were collected every 10 seconds from 8 to 15.5 min and dried in a SpeedVac (SPD-1010-115, Thermo Fisher). Rhamnolipid methyl esters did not exhibit a strong UV absorbance profile ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were cultured routinely in feeder-free conditions in either Essential 8 (Thermo Fisher or made in-house) or mTeSR1 (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... OCT-embedded Brugia malayi adult female worm pieces (see section “Parasite material and treatment”) were cryosectioned longitudinally to 8 µm thickness with a CryoStar NX70 cryostat (ThermoFisher). Samples BM1 and BM2 were sectioned with the re-embedding technique described in the section “Total RNA extraction - RNA quality” while sample BM3 was sectioned from the original OCT block ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Developmental Biology 2022Quote: Kc167 cells (8×105 cells per well) were co-transfected in the presence of 10% Foetal Bovine Serum (Cat. # 10500064, Gibco) in 6-well plates with pActin-Gal4 and pUAS-Lgr4 vector using Fugene-HD (Cat ...
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bioRxiv - Genomics 2023Quote: ... AMD associated SNPs that were not present on the HumanOmni2.5-8 BeadChip were genotyped using a combination of pre-designed and Custom Taqman SNP Genotyping Assays (Applied Biosystems). Each assay was run in a 15 µl reaction containing 2x Taqman GTXpress master mix ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated at a concentration of 3,000 neurons/well in Matrigel coated Lab-Tek II 8-well chamber slides or 24-well plates (Thermo Fisher). TGs were collected after separation from debris via density gradient centrifugation using OptiPrep (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... S2 cell pellets from 200 ml of culture were resuspended with 16 ml lysis buffer (50 mM Tris, pH 8, NaCl 150 mM, 20% glycerol, 1x protease inhibitor (Cat# A32965, ThermoFisher)) ...
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bioRxiv - Developmental Biology 2023Quote: ... alginate and calcium sulfate solution were either directly deposited into an 8-well Lab-Tek chamber slide (Thermo Fisher Scientific) or onto a hydrophobic glass plate which was then covered with another glass plate with a 1 mm spacer between plates ...
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bioRxiv - Genomics 2023Quote: ... Long term cultured transduced cells were seeded at 10,000 cells per cm2 in 8 well Permanox Nunc Lab-Tek chambers (ThermoFisher #177402) and grown for 5 days in growth medium ...
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bioRxiv - Biophysics 2023Quote: ... Then we detached them using trypsin and plated a 1:10 dilution in DMEM on an 8-well chamber (Lab-Tek 1.0 borosilicate glass, Thermo Fisher), which we let grow overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Stably transduced cell lines were generated by infection over-night in the presence of 8 μg/ml polybrene and 48 hours after infection were selected with 1 μg/mL Puromycin (Gibco) for three days ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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bioRxiv - Microbiology 2023Quote: Expi293F / 293F cells were maintained at 37 °C and 8% CO2 in Expi293 / 293F freestyle Expression medium (Gibco; ThermoFisher Scientific) with continuous shaking at 110-130 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
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bioRxiv - Physiology 2023Quote: Animals were culled using a schedule-1 method followed by injection of the common bile duct with 1 mg/mL collagenase NB 8 (Serva) in RPMI 1640 (Gibco) and pancreas dissection ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured at 80 °C for 8 minutes in LDS sample buffer containing 1x NuPAGE reducing agent (ThermoFisher Scientific). Samples were run on a NuPAGE 4-12 % Bis-Tris gel (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: Approximately 8·106 HEK-293T cells were seeded into T75 flasks previously coated with poly-D-lysine (Gibco™ A3890401). After 16 - 24 h (when cells reached around 90% confluence) ...
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bioRxiv - Microbiology 2023Quote: Expi293F / 293F cells were maintained at 37 °C and 8% CO2 in Expi293 / 293F freestyle Expression medium (Gibco; ThermoFisher Scientific) with continuous shaking at 110-130 rpm ...
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bioRxiv - Genomics 2023Quote: ... these reactions were electrophoresed on a non-denaturing 8% PAGE gel alongside O’RangeRuler 10 bp DNA and GeneRuler 50 bp DNA ladders (Thermo Fisher), followed by SYBR gold staining ...
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bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...