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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... colonoids were dissociated using TrypLE and replated in an 8-well glass-bottom chamber slide (Nunc™ Lab-Tek™, Cat#155411). Three to four days later ...
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bioRxiv - Cell Biology 2021Quote: ... On-feeder hiPSC cultures were gradually transitioned to feeder- free conditions by weaning off from the standard HuES media to Essential 8™ medium (Gibco, #A1517001) and maintained on hESC-qualified Matrigel coated surface ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 1X Phosphatase inhibitor (Thermo Scientific, #1862495)) at room temperature for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Cell Biology 2022Quote: ... Human iPSC lines were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium (Thermo Fisher Scientific) supplemented with Essential 8 Flex Supplement (50x ...
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bioRxiv - Molecular Biology 2022Quote: ... The retinas were washed in PBS for 30 min at least 8 times at room temperature and incubated with anti-IB4 (I21412, Invitrogen, 1:200) and Alexa Fluor 488 goat anti-rabbit IgG (A11008 ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were killed with 10 μL of 6X SDS loading buffer and pooled or directly loaded onto a 15-well Novex Wedgewell 8% Tris-Glycine Gel (Thermo Fisher Scientific) and ran at 175 V for ~ 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures for immunocytochemistry and RNAscope experiments were prepared as described above with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek). The neurons were resuspended in culture media ...
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bioRxiv - Neuroscience 2019Quote: Wild-type C57/BL6J mice of either gender (aged 8-10 weeks) were used as recipients of yellow-green microsphere (polystyrene FluoSpheres™, 505ex/515em nm, Invitrogen, USA) transplantation ...
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bioRxiv - Cell Biology 2021Quote: ... enzyme activity was stopped with 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA). The digestion mixture was passed through 100 μm cell strainer and centrifuged at 150 x g for 8 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: The human embryonic stem cell (hESC) line Hues9 (H9) was obtained from the Wicell Research Institute (Madison, WI) and maintained in Essential 8 media (Thermo Fisher Scientific) on hESC-Qualified Matrigel (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were boiled at 95°C for 5 min before separating on Novex WedgeWell 8–16% Tris-Glycine Mini Gels (Thermo Fisher Scientific). Protein samples were transferred to polyvinylidene fluoride membrane (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...
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bioRxiv - Cell Biology 2021Quote: The tomograms showed in the Figure 8 and 9 were performed on Glacios equipped with a field emission gun and operated at 200 kV (Thermo Fisher Scientific) and a Falcon 3 direct electron detector ...
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bioRxiv - Microbiology 2021Quote: ... mutations were inserted in fragment 8 (Extended Data Table 3) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, cat# A13312) according to the manufacturer’s protocol with the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies, Carlsbad ...
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bioRxiv - Developmental Biology 2022Quote: ... for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201) for 30 minutes at 37C ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
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bioRxiv - Microbiology 2022Quote: Mammalian cells were seeded at a density of 8×105 in a 96-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2022Quote: ... templated and sequenced using either Ion 550™ Kit-Chef and Ion 550 Chip Kit as one 16-plex library pool or Ion 540™ Kit-Chef and Ion 540 Chip Kit as one 8-plex library pool (Thermo Fisher). Two independent sequencing analyses were performed on a panel of eight samples ...
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bioRxiv - Cell Biology 2019Quote: ... placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12) and covered with the supplied top (Thermo Fisher Scientific). The covered tubes in racks were transported to a Gammacell 40 137Cs irradiator (Atomic Energy of Canada Ltd. ...
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bioRxiv - Biophysics 2019Quote: iPSCs were cultivated for several passages under feeder-free conditions (in the Essential 8™ Medium on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific). 3-4 days before differentiation ...
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bioRxiv - Biophysics 2019Quote: ... iPSCs were cultured in the Essential 8™ Medium on a Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) after transferring from the feeder layer ...
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bioRxiv - Biophysics 2019Quote: ... Cells were seeded 1 or 2 days prior the experiment at low density (10000-12000 cells per well) in a glass bottom 8-well Lab-Tek II chambered coverglass (Thermo Scientific Nunc, 155383).
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bioRxiv - Synthetic Biology 2019Quote: ... the AAV helper plasmid carrying the rep and cap genes of AAV serotype 2 and (iii) an adenoviral plasmid providing helper functions for AAV production using 8 μl TurboFect Transfection Reagent per well (Thermo Fisher Scientific). 72 hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells or EphA2 HEK293T stable cells were seeded (1 x 104 cells/well) into 8-well tissue culture chambered coverglass slides (Thermo Scientific; 12565338) and cultured for 36 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... The following media was supplied by a peristaltic pump set to deliver 8 ml/min volume: 150 μl wheat germ agglutinin conjugated to Alexa 594 dye (ThermoFisher Scientific, W11262) and 15 ml PBS 1x and 15 ml 4% PFA.
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bioRxiv - Cell Biology 2021Quote: Proteins were separated by SDS-PAGE (8 % gels) and then transferred to a nitrocellulose membrane with an iBLOT 2 Gel Transfer Device (ThermoFisher Scientific, IB21001). Nitrocellulose membranes were blocked for 1 h with 5 % dried milk resuspended in TBS-Tween 1 % and incubated over-night with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (Thermo Scientific #90057) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Invasion assays were performed in 24-well Matrigel invasion chambers with an 8 μm pore size polyethylene terephthalate filter coated in Matrigel matrix that separated the upper and lower wells (Thermo Fisher Scientific). In both assays ...
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bioRxiv - Cell Biology 2021Quote: ... Migration assays were performed in 24-well chemotaxis chamber with an 8 μm pore size polyethylene terephthalate filter that separated the upper and lower wells (Thermo Fisher Scientific). Invasion assays were performed in 24-well Matrigel invasion chambers with an 8 μm pore size polyethylene terephthalate filter coated in Matrigel matrix that separated the upper and lower wells (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: AXL −ve and AXL-positive cells from H1650 and PC9 were FACS-sorted and cultured for 2 days in an 8-well chamber slide system (LAB-TEK, Thermo Fisher Scientific). H1650 ...
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bioRxiv - Cell Biology 2019Quote: ... GM2610 and AICS-0023 clone 20) were maintained under feeder-free condition using Essential 8 (E8) medium and vitronectin (VTN-N) (Thermo Fisher Scientific). In some cloning experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... Spermatocytes and spermatids isolated from 8-week-old C57BL/6 mice were suspended in αMEM with 10% knockout serum replacement (Thermo Fisher Scientific) and 10 μg/ml GDNF (Funaskoshi) ...
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bioRxiv - Biochemistry 2021Quote: ... The grid was blotted at 8 ° C and 100% humidity and plunge-frozen in liquid ethane using FEI Vitrobot Mark IV (Thermo Fisher Scientific). Cryo-EM data was collected on a Titan Krios electron microscope operated at an acceleration voltage of 300 kV with the defocus range of −1.0 μm to −2.0 μm using SerialEM software [25] ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were then blotted for 2s at 100% humidity at 8°C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted super-resolution mode on a Titan Krios G3 (FEI ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... These cell lines were kept in maintenance culture with StemFit AK02N medium (Ajinomoto) and then cultured for two passages in maintenance culture using Essential 8 Medium (Thermo Fisher Scientific) as in the case of KhES-3 ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from frozen tissues of Cyperus esculentus mixed tissue sample and 8 different stages of tuber development by homogenizing material in Plant RNA Reagent according to instructions (Invitrogen, Carlsbad, USA). RNA integrity and concentration were determined using Experion RNA StdSens analysis kit (BioRad ...
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bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
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bioRxiv - Plant Biology 2020Quote: A total of 85 μg of protein per sample was subjected to one-dimensional SDS-PAGE using an 8-16% precise protein gradient gel (Thermo Fisher Scientific). Proteins were separated for 1 cm in the resolving gel and the entire lane was excised and cut into equal pieces ...
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bioRxiv - Microbiology 2020Quote: ... HCT-8 human ileocecal colorectal adenocarcinoma cell line and its 5-FU-resistant was cultured in RPMI 1640 medium (Gibco, New Zealand) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... 100 U/ml penicillin,100 μg/ml streptomycin, 8% (vol/vol) fetal bovine serum (all Bio&Sell, Nürnberg, Germany) and 2% (vol/vol) chicken serum (ThermoFisher Scientific, Waltham, USA), with the addition of recombinant soluble chicken BAFF and chicken CD40L ...