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Citations for Thermo Fisher :
1901 - 1950 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Neuroscience 2023Quote: ... SARK and HMW tau extracts (8 ng total tau per well) were incubated with 1% lipofectamine 2000 transfection reagent (ThermoFisher) in opti-MEM (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cortex tissue from up to 8 embryos was pooled into 1.5 mL microfuge tubes with Hank’s balanced salt solution (HBSS) (Gibco -14170-112), rinsed twice with fresh HBSS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were cultured routinely in feeder-free conditions in either Essential 8 (Thermo Fisher or made in-house) or mTeSR1 (Stem Cell Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
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bioRxiv - Microbiology 2023Quote: ... Samples with an RNA Integrity Number (RIN) of 8 or more were used for rRNA depletion using a Ribominus v2 kit (ThermoFisher) and both bacteria-specific and human-specific rRNA probes as per the manufacturer’s instructions (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... OCT-embedded Brugia malayi adult female worm pieces (see section “Parasite material and treatment”) were cryosectioned longitudinally to 8 µm thickness with a CryoStar NX70 cryostat (ThermoFisher). Samples BM1 and BM2 were sectioned with the re-embedding technique described in the section “Total RNA extraction - RNA quality” while sample BM3 was sectioned from the original OCT block ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Developmental Biology 2022Quote: Kc167 cells (8×105 cells per well) were co-transfected in the presence of 10% Foetal Bovine Serum (Cat. # 10500064, Gibco) in 6-well plates with pActin-Gal4 and pUAS-Lgr4 vector using Fugene-HD (Cat ...
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bioRxiv - Genomics 2023Quote: ... AMD associated SNPs that were not present on the HumanOmni2.5-8 BeadChip were genotyped using a combination of pre-designed and Custom Taqman SNP Genotyping Assays (Applied Biosystems). Each assay was run in a 15 µl reaction containing 2x Taqman GTXpress master mix ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated at a concentration of 3,000 neurons/well in Matrigel coated Lab-Tek II 8-well chamber slides or 24-well plates (Thermo Fisher). TGs were collected after separation from debris via density gradient centrifugation using OptiPrep (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... S2 cell pellets from 200 ml of culture were resuspended with 16 ml lysis buffer (50 mM Tris, pH 8, NaCl 150 mM, 20% glycerol, 1x protease inhibitor (Cat# A32965, ThermoFisher)) ...
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bioRxiv - Biochemistry 2023Quote: ... 200 mM Tris-HCl, pH 8.0, 8 M Urea, 4% CHAPS, 1 M NaCl, supplemented with protease and phosphatase inhibitors, Thermo Scientific) was added to the pellets and the extracts were sonicated 10 x 3s with a probe sonicator ...
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bioRxiv - Developmental Biology 2023Quote: ... alginate and calcium sulfate solution were either directly deposited into an 8-well Lab-Tek chamber slide (Thermo Fisher Scientific) or onto a hydrophobic glass plate which was then covered with another glass plate with a 1 mm spacer between plates ...
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bioRxiv - Genomics 2023Quote: ... Long term cultured transduced cells were seeded at 10,000 cells per cm2 in 8 well Permanox Nunc Lab-Tek chambers (ThermoFisher #177402) and grown for 5 days in growth medium ...
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bioRxiv - Cell Biology 2023Quote: Mixed populations of keratinocytes were prepared and seeded onto 8-chamber glass slides with removable wells (Lab-Tek, ThermoFisher #154534). Cells were grown to approximately 50% confluence ...
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bioRxiv - Biophysics 2023Quote: ... Then we detached them using trypsin and plated a 1:10 dilution in DMEM on an 8-well chamber (Lab-Tek 1.0 borosilicate glass, Thermo Fisher), which we let grow overnight ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: Approximately 8·106 HEK-293T cells were seeded into T75 flasks previously coated with poly-D-lysine (Gibco™ A3890401). After 16 - 24 h (when cells reached around 90% confluence) ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were resuspended in 45 μL of 1X Ligase buffer containing 8 nM of adapter and 2.5 units of T4 DNA ligase (ThermoFisher, 15224041) and incubated at room temperature for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 15,000×g for 15 minutes at 4°C (ThermoFisher Fiberlite F15s-8×50c rotor). The cell pellets were washed in 100ml of 30mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cartilage fragments were subsequently cut with an 8 mm diameter disposable biopsy punch (Robbins Instruments) and cultured for 24h in DMEM/F12 (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Expi293F / 293F cells were maintained at 37 °C and 8% CO2 in Expi293 / 293F freestyle Expression medium (Gibco; ThermoFisher Scientific) with continuous shaking at 110-130 rpm ...
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bioRxiv - Cancer Biology 2023Quote: 1.0×104 of PDAC cells were seeded on 8-well NuncTM Lab-TekTM II CC2TM Chamber Slides (Thermo Fisher Scientific). Cells were washed with PBS and fixed in 4% paraformaldehyde in PBS at room temperature for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168; PC Bottle Assembly 70 mL, ThermoFisher) for 1 hour at 4° C ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured at 80 °C for 8 minutes in LDS sample buffer containing 1x NuPAGE reducing agent (ThermoFisher Scientific). Samples were run on a NuPAGE 4-12 % Bis-Tris gel (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Biochemistry 2024Quote: ... The uptake reaction was stopped after 8 min by washing the cells thrice with ice-cold phosphate-buffered saline (PBS, Gibco). Lysis buffer containing 1 M NaOH and 2% Triton X-100 in H2O was added ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000g for 30 min at 4°C with a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... the medium of the electroporated U2OS cells on the 8-well microscopy slides was exchanged with 300 µl of fresh medium containing 500 ng/ml Hoechst33342 (H3570; Invitrogen) and no phenol red ...
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bioRxiv - Microbiology 2023Quote: Expi293F / 293F cells were maintained at 37 °C and 8% CO2 in Expi293 / 293F freestyle Expression medium (Gibco; ThermoFisher Scientific) with continuous shaking at 110-130 rpm ...
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bioRxiv - Physiology 2023Quote: Animals were culled using a schedule-1 method followed by injection of the common bile duct with 1 mg/mL collagenase NB 8 (Serva) in RPMI 1640 (Gibco) and pancreas dissection ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... these reactions were electrophoresed on a non-denaturing 8% PAGE gel alongside O’RangeRuler 10 bp DNA and GeneRuler 50 bp DNA ladders (Thermo Fisher), followed by SYBR gold staining ...
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bioRxiv - Molecular Biology 2024Quote: ... The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG) – a proxy for oxidative DNA damage – was analyzed in corals using DNA Damage Competitive ELISA Kit (Invitrogen) and MyCurveFit.com software as described in (20).