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Citations for Thermo Fisher :
1851 - 1900 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The tubes were then supplemented with 630 μl chloroform/isoamyl alcohol (24:1, pH 8, Acros Organics, Cat. No.: 327155000) and bead beat at setting 6 for 40 sec using the FastPrep-24™ Classic Instrument (MP Biomedicals ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... 72 µg PE-Puro or PE-FeLV plasmid were mixed with 8 µg pCS2-GFP and 40 µl Lipofectamine P3000 (Invitrogen) in 3.2 ml Opti-Mem (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... The mixed cell suspension was dispensed into a large dimple plate (Tissue by Net Ltd., Tokyo, Japan) for mixed spheroids formation in Essential 8 Medium (A1517001 Invitrogen) for four days before being collected and transferred to a 3D printed mold ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: Murine MC38-RFP adenocarcinoma cells and MC38 hybrid cells (MC38-macrophage hybrid cells)8 were cultured in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and embedded in Tissue-Tek O.C.T Compound (Sakura) and cut into 8 μm sections using CryoStar NX50 (Thermo fisher Scientific). Slides were permeabilized with 0.3% Triton X-100 (Beyotime ...
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bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... cells were plated in three biological replicates in an 8-well Lab-Tek II chambered cover-glass system plate (Nunc) or 24-well plates at a density of 100,000 cells/well in 500 µL of media and incubated at 37 °C under a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Jupiler with increased ethanol levels (8% and 13.5% ABV) (adjusted using 100% ethanol, Fisher Chemical, Thermo Fisher Scientific, Waltham, USA); dry-hopped Jupiler (using Cascade Cryo Hops® pellets ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α) were measured using a custom 8-plex panel mouse ProcartaPlex assay (ThermoFisher Scientific, catalog# PPX-08-MXGZGFX), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... for a total of three replicates per condition and fragmented for 8 minutes at 70°C to an average fragment size of ~80 nt using Fragmentation Reagents (Invitrogen). rRNA depletion ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Microbiology 2022Quote: ... To prepare CONV larvae first instars from surface-sterilized eggs were exposed immediately upon hatching to CONV inoculum at selected dilution (from 10−3 to 10−8) in sterile water (Gibco). Bacterial composition of CONV inoculum is provided (FIG S2 ...
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bioRxiv - Molecular Biology 2022Quote: Live cell imaging of fluorescent cells was carried out in Nunc Lab-Tek II 8 well chambered coverglass in Live Cell Imaging Solution (Invitrogen) using a 40x EC PlnN oil objective with NA=1.3 on a Zeiss AxioObserver Z1 with an AxioCam MRm monochrome digital camera ...
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bioRxiv - Molecular Biology 2023Quote: Proliferating C2C12 myoblasts were transduced with lentivirus in the presence of 8 µg/ml polybrene and selected with 2 µg/ml puromycin (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Cells were mounted on microscopy slides using GLOX anti-fade buffer containing 10 mM Tris pH 8 (Thermo Fisher, AM9856), 2x SSC (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... 50-100 μg of protein in 10-40 μl volume was electrophoresed through 8% or 12% Bolt Bis-Tris Plus gels (InVitrogen) in Bolt MOPS or MES SDS running buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded as single cells at a concentration of 200K cells/cm2 in maintenance medium (Essential 8 medium, Thermo Fisher) supplemented with a rho kinase inhibitor (Stemgent ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Neuroscience 2022Quote: ... in EER mode with a flux of 8 electrons/pixel/s and a Selectris-X energy filter (Thermo Fisher Scientific) with a slit width of 10 eV to remove inelastically scattered electrons ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... selection for infected cells was initiated through the introduction of 2mg/mL (hTR-8/SVneo) or 8mg/mL (HepG2) puromycin (Gibco). Once stably infected ...
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bioRxiv - Plant Biology 2022Quote: ... 8 μm paraffin sections were prepared using a Leica rotary microtome RM2265 and transferred to polysine coated slides (ThermoFisher Scientific), dewaxed ...
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bioRxiv - Neuroscience 2023Quote: ... the hippocampus form 6-8 neonatal mice was collected from both hemispheres in Hank’s buffered saline solution (HBSS, Life Technologies). Tissue was incubated in 0.25% trypsin (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... malayi adult female tissue (see section “Parasite material and treatment”) was cryosectioned longitudinally to 8 µm thickness with a CryoStar NX70 cryostat (ThermoFisher). All sections (∼11-16 sections per block ...
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bioRxiv - Immunology 2023Quote: ... were distributed into four wells of a 24-well plate and activated for 8 days by adding prewashed anti-CD3/CD28 Dynabeads at a 1:1 bead-to-cell ratio (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Genomics 2022Quote: ... and resuspended in 1x lysis buffer (10mM Tris pH 8, 5mM EDTA, 0.5% SDS, 1x (0.4mg/ml) Proteinase K (Thermo Fisher Scientific) in water at 10M cells/800ul ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: DRG were dissected from 8- to 10-wk-old C57BL/6J mice in cold Hank’s balanced salt solution (HBSS) (Life Technologies) or Dulbecco’s Modified Eagle’s medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... embryonic fetal lung lobes were incubated in Advanced DMEM/F12 medium supplemented with 8 U/ml Dispase (ThermoFisher Scientific, Gibco) for 2 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... were then seeded at a density of ~8 × 104 cells/cm2 in EpiLife medium containing 60 µmol/L CaCl2 (#MEPI500CA Gibco) and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Microbiology 2023Quote: ... Samples with an RNA Integrity Number (RIN) of 8 or more were used for rRNA depletion using a Ribominus v2 kit (ThermoFisher) and both bacteria-specific and human-specific rRNA probes as per the manufacturer’s instructions (Life Technologies) ...
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bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... SARK and HMW tau extracts (8 ng total tau per well) were incubated with 1% lipofectamine 2000 transfection reagent (ThermoFisher) in opti-MEM (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cortex tissue from up to 8 embryos was pooled into 1.5 mL microfuge tubes with Hank’s balanced salt solution (HBSS) (Gibco -14170-112), rinsed twice with fresh HBSS ...
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bioRxiv - Biophysics 2023Quote: ... samples were mixed with 2X Novex Tris-glycine native sample buffer and loaded into NuPAGE 3-8% Tris-glycine gels (Invitrogen) with NativeMark unstained protein standard (Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... We section organoid blocks at 8 µm and mount them on Superfrost Plus Microscope Slides (12-550-15, Fisher Scientific). Hematoxylin and eosin staining was performed on the parent tumor and the resulting organoids derived from tissue collection according to standard protocols ...