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Citations for Thermo Fisher :
1801 - 1850 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Systems Biology 2022Quote: ... The consistency of plasmid expression throughout different experiments was ensured by transfecting 80 ng of each plasmid per well of an 8-well Lab-Tek™ chambered cover glass slides (0.8 cm2 surface area, ThermoFisher) and the amount of plasmid was scaled up proportional to the culture-well surface area for other culture-plates used ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown for six days at 37 °C with 8% CO2 shaking 125 rpm according to the manufacturer’s protocol (Thermo Fisher). Cell cultures for Spike-W and Spike Omicron BA.1 and BA.2 were harvested six days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Molecular Biology 2022Quote: ... Cas9 protein was prepared by mixing 12 μl of Alt-R Cas9 protein (62 μM) with 8 μl of buffer R (Invitrogen). gRNA duplexes and Cas9 were then mixed gently and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2019Quote: ... dorsal root ganglion neurons were trimmed and isolated from postnatal 8-10 day SD rat spinal cords in HBSS (Invitrogen), and digested in 2.5 U/ml dispase II (Roche ...
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bioRxiv - Molecular Biology 2019Quote: Samples were loaded onto 8% SDS-polyacrylamide gels or 4–12% Tris/HEPES-SDS Bolt™ precast gels (Thermo-Invitrogen) for electrophoresis ...
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bioRxiv - Biophysics 2019Quote: QDs were functionalized with benzyl guanine by mixing 5 μl of 8 μM amino (PEG) QDs emitting at 655 nm (ThermoFisher) with 2 μl of 20 mM BG-GLA-NHS (NEB ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing G12V Rac1A under a tetracycline-inducible promoter along with GFP-WASP or RFP-tagged active Rac marker were resuspended in LoFlo and seeded to a final concentration of 5×105 cells/ml on a borosilicate glass 8 well chamber (ThermoFisher) or on a 35 mm glass bottom dish (MatTek) ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Genetics 2019Quote: ... 10 or 15μg of protein was loaded per well on Tris-Acetate 3-8% gels with Tris-Acetate running buffer (NuPAGE, ThermoFisher) for detecting KIF26B protein or on 4-12% NuPAGE Bis-Tris Gel with NuPAGE MES SDS Running Buffer (NuPAGE ...
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bioRxiv - Cell Biology 2019Quote: MDA-MB-468 cells were grown to 70% confluence on 8-chambered glass slides (Nunc Lab-Tek II, Thermo Scientific), starved in serum-free media for 24 h,and were treated with DMSO carrier or SHP099 (10 uM ...
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bioRxiv - Cell Biology 2019Quote: MDA-MB-468 cells were grown to 70% confluence on 8-chambered glass slides (Nunc Lab-Tek II, Thermo Scientific), starved in serum-free media for 24 h,and were treated with DMSO carrier or SHP099 (10 uM ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and 64 °C) for 8 min using a VeriFlex temperature control technology of the thermocycler (Applied Biosystems VeritiTM Thermal Cycler). Immediately ...
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bioRxiv - Pathology 2020Quote: Stored blood samples collected on d 9 from selected 8 NEC and 8 healthy pigs across 3 litters were used for RNA extraction using MagMax 96 blood RNA isolation kit (Thermofisher) for transcriptomics by whole transcriptome shotgun sequencing as previously described24 ...
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bioRxiv - Developmental Biology 2020Quote: ... We analyzed 40 μg of protein by SDS-polyacrylamide gel electrophoresis on an 8% Tris-Glycine gel (Thermo Fisher Scientific) and transferred overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The lines were grown and expanded in feeder-free conditions on vitronectin using Essential 8 plus medium (Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... On day 3 the cells were transfected with 60 pMol of either miR-122 or control microRNA (miR-122 p2-8) or anti-miR-122 LNA or control LNA (anti-miR-124) with 5uL of Lipofectamine 2000 (Invitrogen). Cells were harvested 2 days post-transfection and luciferase activity was measured.
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bioRxiv - Cancer Biology 2020Quote: ... The cell culture supernatant was collected and used for quantifying IL-8 secretion using the ELISA assay kit (# 88-8086-88, ThermoFisher). The assay was performed following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 5×103 RAW 264.7 cells were seeded per well in a Nunc Lab-Tek II 8-well Chamber Slide system (ThermoFisher Scientific) in a total of 300 μl osteoclast differentiation medium containing 30 ng/ml RANKL ...
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bioRxiv - Microbiology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of whole protein extracts from thymocytes and 50μl from myeloid cells were loaded on pre-cast NuPAGE™ BOLT 8% Bis-Tris polyacrylamide gels (Invitrogen) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were transfected with 8μl (10 μM) small interfering RNA (sc-43983, Sant Cruz Biotechnology) with 8 μl Lipofectamine™ RNAiMAX (Invitrogen) for 6 hours according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1×103 cells were seeded per well in either a 48-well or 8-chamber format in DMEM high glucose (Gibco) and refreshed every 3 to 4 days.
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The cells were transfected in triplicate with 10 μg of each bacmid DNA (bAcac13KO-ph, bAcac13REP-ph and bAc-ph)using 8 μL of Cellfectin II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the mouse Achilles tendon samples were stained with 0.2% of 4 mg/ml fluorescein diacetate (FDA) (596-09-8, ACROS Organics) and 0.5% of 1 mg/ml propidium iodide (PI ...
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bioRxiv - Immunology 2021Quote: HEK203-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2020Quote: HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Plant Biology 2019Quote: ... as well as custom pENpAtPAD4 StrepII-YFP (Supplemental Dataset 8 with sequence in.gbk format) was performed using LR Clonase II (11791100, Life technologies).
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bioRxiv - Cell Biology 2021Quote: RBL-2H3 cells in a confluent 75 cm2 flask were washed and trypsinized for 8 min at 37 °C with 3 mL of 0.05% Trypsin-EDTA (Thermo Fisher). The detached cells were resuspended in 7 mL of growth medium and centrifuged to remove the medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Cell Biology 2020Quote: ... pALN25: GFP-BUB1-T609A) and selected with 2 mg/ml (HeLa) or 8 mg/ml (K-562) Blasticidin (Life Technologies) (Cheeseman and Desai ...
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bioRxiv - Cell Biology 2020Quote: ... The cell pellet was resuspended in SFSC medium and 50,000 cells were seeded into each well of 8 well Lab-Tek II glass chamber slides (Nunc, 154534) for immunofluorescence preparation.
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bioRxiv - Cell Biology 2020Quote: ... of cell lysate proteins per lane were resolved on SDS-PAGE (8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 1 hour using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IPSCs were maintained on human Laminin-521 (BioLamina, Sundbyger, Sweden) coating in essential 8 (E8) medium (DMEM/F12 supplemented with 15 mM Hepes (Gibco/Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were grown until 70% confluency before transfection in 8 well Nunc Lab Tek II chambered slides with 0.16 mm cover glass thickness from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Neonatal rat ventricular myocytes were plated at a density of 0.5 × 106 cells per well on fibronectin coated 34.8 mm plates and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 1:1 (cat# 11330-032, Thermo Fisher Scientific), containing 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: Immunoprecipitations for competition assays were performed with 8 μg of C-terminally biotinylated nanobody on M-280 streptavidin magnetic beads (Dynabeads, Invitrogen) and 10 μg of spike or RBD preincubated with 10 μg of the indicated (HIS-tagged ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... containing 50 mM dithiothreitol (DTT) for 5 min at 100°C before loading on 8-16% Tris-glycine gels (ThermoFisher). Gels were electrophoresed at RT in running buffer (25 mM Tris ...