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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Dengue viruses were grown in C6/36 cells cultured in RPMI 1640 (Gibco 11875-093), L-Glutamine (Gibco 25030-081) ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The lentiviral envelope vector pLP/VSVG (Invitrogen) and the packaging vector psPAX2 (Trono lab ...
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bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Biochemistry 2019Quote: Antibodies and antibody Fabs were produced as described previously.57 BG505 N332 SOSIP gp140 envelopes were expressed as previously described with minor modifications.58 Envelope production was performed with Freestyle293 cells (Invitrogen). On the day of transfection ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... the VSV-G envelope protein vector pMD2.G and the respective pGIPZ-shRNA-target plasmid using lipofectamine 3000 (Invitrogen #L3000008). Filtered virus supernatant was used to transduce MDA-MB-468 and 4T1 cells in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... and 260 ng pMD.G envelope plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25 µg envelope plasmid diluted in 100 µl optiMEM (Gibco) was mixed with 20 µl of PolyFect transfection reagent (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Biophysics 2022Quote: ... and pCMV-VSV-G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2024Quote: ... and 2 U/µl of M-MulV-RT (Reverse transcriptase of Moloney Murine Leukemia Virus; Thermo Scientific, USA).
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...