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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 37°C 5% CO2 in DMEM and then cells were reverse transfected with 2 μg of GFP using lipofectamine2000 (Invitrogen) in 6 well plate.
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bioRxiv - Immunology 2021Quote: ... samples were incubated for 30 minutes at 4°C with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher Scientific) while in PBS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... cells were centrifuged for 5 min at 1400 rpm and incubated at 37°C for 2 min in sterile RBC lysis solution (Invitrogen). Lysis was arrested by adding 30 ml PBS ...
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bioRxiv - Immunology 2019Quote: ... and a final 10min 68°C extension) from 2-3μl of cDNA in 25μl of Accuprime Pfx supermix (Invitrogen, Thermo Fisher Scientific) and 0.5μl of the VH ...
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bioRxiv - Molecular Biology 2019Quote: ... 3’-linked footprints were denatured at 80°C for 2 min and purified on 10% TBE-Urea polyacrylamide gels (Invitrogen) in 1x TBE (Ambion ...
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bioRxiv - Immunology 2020Quote: ... for 2 h at 37°C and 5% CO2 in the presence of dextran-rhodamine (0.5 mg/mL) (Molecular Probes). mAb-untreated Pf sporozoites were used to measure the maximum traversal capacity ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in 2% BSA/PBS were incubated overnight at 4°C and secondary antibodies (Thermo Fisher Scientific, Alexa Fluor) were incubated for 2 hours at room temperature and washed with PBS-T ...
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bioRxiv - Immunology 2019Quote: ... 100µl cell suspension was embedded by centrifugation (5’ at 1230 g at 30°C) in 100µl 2% Low Melting Agarose (LMA, Gibco BRL) in 0.1M cacodylate buffer ...
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bioRxiv - Cancer Biology 2020Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/mL) (Invitrogen), resuspended in DMEM (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with PBS and incubated for 2 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing 5 or 2.5 μM remdesivir alone or in combination with 10 μM ramelteon (PubChem ID 208902) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting PCR product (N linker::CeTurboID::c-Myc::mNeongreen::3XFLAG) was cloned into position 2 of a Multisite Gateway pDONR vector (ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... Retinas were maintained in culture (37°C, 5% CO2) fed by Neurobasal-A media supplemented with 2 mM L-glutamate (GlutaMAX, Gibco) 2 % B27 ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RT mixtures were incubated at 52°C for 2 min before addition of 1 μL (200 U) of Superscript III Reverse transcriptase (Invitrogen) and reactions were incubated at 55°C for 2 hr ...
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bioRxiv - Immunology 2021Quote: Serum samples were heat-inactivated for 30 minutes at 56°C and diluted in a 2- fold serial fashion in MEM (Gibco) with Hepes (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 ml cells were pre-incubated at 18 °C for 2 h and fixed with 1.45% Formaldehyde from 16% stock (ThermoFisher 28908) for 30 min at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then incubated overnight at 4°C and under agitation with 2 mL Ni-NTA resin (50% v/v, Thermofisher) pre-equilibrated with lysis buffer.
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bioRxiv - Immunology 2021Quote: ... the eWAT was minced and incubated for 35 min at 37°C under gentle agitation (150rpm) in cRPMI (2% BSA FFA-free [Fisher BP9704100], 1mM of Sodium Pyruvate [Gibco, 11360-070] ...
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bioRxiv - Immunology 2021Quote: ... in 96- or 24-well flat bottom plates pre-coated for 2 hr at 37 °C with goat-anti-hamster whole IgG (50 μg/mL; Invitrogen). Activation was induced by adding hamster-anti-mouse CD3ε (0.3 or 1 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 10’000 rcf for 30 min and then ultracentrifuged at 120’000 rcf at 4 °C for 2 h (TH 64.1 rotor, Thermo Fisher Scientific). The epididymosomal pellet was then washed in PBS at 4 °C and ultracentrifuged at 120’000 rcf at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and then treated with 0.4 mg/ml proteinase K (1-2 hour, 50°C) before addition of 25 μM Sytox Green (ThermoFisher Scientific). Cells were then sonicated and DNA-content was analysed for >10,000 events on either a BD Biosciences FACScan Analyzer or an Attune NxT flow cytometer ...
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bioRxiv - Molecular Biology 2022Quote: ... PABP-GFP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-GFP (clones 19F7 and 19C8 ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Cancer Biology 2022Quote: ... vials were placed in placed in a 37°C water bath and the contents washed twice in DMEM + 2% FBS (Invitrogen), before reseeding in Matrigel at required density.
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bioRxiv - Developmental Biology 2022Quote: ... tissues were washed in PBS with 2% Triton X-100 at 37°C for 5 days and mounted in SlowFade Diamond Antifade Mountant (S36972, Invitrogen). The stained whole mounts were imaged under the two-photon microscope.
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bioRxiv - Cell Biology 2022Quote: ... ATCC® CCL-2) were grown at 37 °C under 5% CO2 in DMEM high glucose glutamax (Gibco, 61965-059) supplemented with 10% FBS ...
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bioRxiv - Genomics 2022Quote: ... Cells were FACS sorted in 384-well plates and lysis of individual cells was performed for 2 h at 55°C using Proteinase K (Ambion) in 1x Cutsmart (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The resulting pellets were resuspended in 2 mls PBS and dialyzed overnight at 4°C in 1L PBS using SnakeSkin 10,000 MWCO dialysis tubing (Thermo Fisher). Buffer was refreshed and samples were dialyzed again overnight ...
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bioRxiv - Cell Biology 2022Quote: ... MBL) and an additional 2 h at 4°C with 20 μL of a slurry of IgG-conjugated Dynabeads (Invitrogen). After washing the beads five times with IP buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and grown to an OD600 range of 2-4 at 30°C in a deep-well 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Immunology 2023Quote: ... for 2 h at 37 °C and 5% CO2 in the presence of 0.5 mg/ml dextran-rhodamine (Molecular Probes). Untreated Pf sporozoites were used to measure maximum traversal capacity ...
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bioRxiv - Microbiology 2023Quote: ... 0.1 – 100 ng total DNA for the WT NF54 or clonal cyt c-2 KO sample was added to PowerUP SYBR Green Master Mix (Invitrogen) and amplified in duplicate using primer sets specific to the 5’ and 3’ ends of the WT (5’ ...
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bioRxiv - Microbiology 2023Quote: ... Synthesis of cDNA was performed for 10 min at 37°C after addition of 2 µl MMLV-Mix (VD+Mg2+ buffer, BSA, dNTPs and 800 U MMLV reverse transcriptase (Invitrogen). The Reaction was stopped by addition of formamide loading dye and boiling at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... 500µl of the reaction mixture was added to the cells, incubated for 4h at 37°C, and then overlaid with 2% bactoagar (214010, Becton, USA) in MEM (2X, Gibco). Plaque formation was allowed for 36-48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Domes were polymerised by incubation for 15 minutes at 37°C and Advanced DMEM media supplemented with 1x N-2 (Gibco), 1x B-27 (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen; AM8669]). The probe was added to hybridization buffer and hybridized to the blot overnight at 38 °C ...
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bioRxiv - Cell Biology 2023Quote: ... were seeded in the wells of a 24-well plate 48 h before the experiment and incubated at 37°C in 2 ml HepG2 cell culture medium (Advanced DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was DNase digested for 30 min at 37°C using 1 µl TURBO DNase (2 U/µl) (Invitrogen). A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: Bacterial cultures were inoculated from freezer stocks in 2 mL of LB and incubated overnight at 37°C with continuous shaking (250 r.p.m.; MaxQ 8000, Thermo Fisher Scientific). Then ...
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Genetics 2023Quote: Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Cancer Biology 2023Quote: BE(2)-C (ATCC, CRL-2268) cells were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM; Invitrogen; Product number 77749) in IMDM+0.1% BSA ...