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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated for 2 h at 37°C in CRB containing 50 µM of tBOC-Leu-Met-CMAC (Molecular Probes), a calpain-specific substrate ...
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bioRxiv - Genomics 2019Quote: ... All plates were sealed with breathable membranes (Breathe-Easy) and incubated at 30°C in a thermostated incubator (Cytomat 2, Thermo Scientific) with continuous shaking ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block ...
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bioRxiv - Genetics 2021Quote: ... Biotin was added and the reaction was incubated at 37°C for 2 hours prior to capture on streptavidin-coupled dynabeads (Invitrogen, 65001). Enriched DNA was released from the beads during a 2 hour incubation at room temperature with 100mM DTT (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Eluting peptides were converted to gaseous ions via a fused silica Picotip 5 cm x 10 μm ID emitter (NewObjective) for ESI (spray voltage, 2 kV; heated capillary, 300 °C) using a nano-electrospray source (Thermo Scientific). MS spectra were acquired in the positive ion mode on the Orbitrap Fusion MS with an m/z range of 375–1,700 with R of 120,000 at m/z 200 ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 ul/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in FACS buffer (PBS with 2% newborn calf serum ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VIPR particles were centrifuged at 14,500 xg for 3 minutes at 4°C and washed three times in PBS while the beads were immobilized in an Invitrogen DynaMag-2 magnet (Thermo Scientific). VIPR particles were resuspended in sterile PBS at a concentration of 5×108 particles per ml and stored at 4°C.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Neuroscience 2022Quote: ... we used bath applications of waterborne copper sulfate (CuSO4, 50 µM in system water or 10 µM in E3 medium, for 2 hours at 28.5°C, Acros Organics, 10413465) to achieve cell-specific ablation or BAPTA (5 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of sample were loaded onto the trapping column (Acclaim PepMap RSLC, 100 μm × 2 cm, nanoViper, 5 μm 100Å C 18 particles, Thermo Scientific) using 0.1% acetic acid at a flow rate of 7000 nl/min for 3 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The Hela cell line was provided by HeLa CRM-CCL-2™ and maintained at 37°C with 5% CO2 in Dulbecco’s high glucose modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... in a humidified incubator at 37 °C in a 5% CO2 atmosphere in wells containing Neurobasal medium supplemented with 2% B27 (Thermofisher #17504044), 1% N2 (Thermofisher #17502048) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Biochemistry 2023Quote: ... UV-crosslinked at 120 mJ and blocked for 2 hours rotating at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Thermo Fisher). Blots were probed rotating overnight at 42°C with 32P-labeled DNA probes prepared as described below ...
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bioRxiv - Biochemistry 2023Quote: ... The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher). Blots were incubated with 10 mL of the resuspended probe overnight at 42°C.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated at 37°C with shaking for exactly 2 min and immediately supplemented with 40 U of RNase inhibitor (RiboLock RNase Inhibitor, ThermoFisher Scientific) followed by 3 min incubation on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the cell homogenates were stored at -80°C for 2 hours and dried down overnight in a Savant SpeediVac (Thermo Scientific). Sample order was randomised and lipids were extracted using a single-phase butanol/methanol extraction method ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: Peptides were desalted for 4 minutes on a trap column (1 mM ID x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated on a C18 analytical column (Waters Acquity UPLC BEH C18 Column ...
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bioRxiv - Molecular Biology 2023Quote: ... Sf21 cells were grown to a density of 2 × 106 cells/ml at 27 °C in Sf900II medium (Thermo Fisher Scientific), infected with the V1 UNK stock of baculovirus ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Biophysics 2024Quote: ... and desalted onto a trap column (1 mM inner diameter x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated onto a C18 analytical column (Waters Acquity UPLC BEH ...
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bioRxiv - Cell Biology 2024Quote: ... on a Thermo Easy-Spray PepMap RSLC C18 75 µm x 50 cm C-18 2 µm column (Thermo Scientific™) with a step gradient of 4–25% solvent B (0.1% FA in 80 % ACN ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubating at 55°C for 20 minutes with reaction termination upon addition of 2 μL of 0.2% SDS (Invitrogen, 15553-035) at room temperature for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER rolls and unrolled control samples were incubated at 37 °C in complete organoid media with 50 μM of 5-Iodo-2’deoxyuridine (IdU, Fisher Scientific) and 20 uM of Telox64 (courtesy of Mark Nitz ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...
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bioRxiv - Cell Biology 2021Quote: CFAP61 was immunoprecipitated from mouse testis using the Pierce crosslink IP kit (Thermo Scientific) with anti-CFAP61 antibody described above ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from frozen mouse tissues using the Purelink RNA Extraction Kit (Invitrogen). Tissues were homogenized with a rotor-stator homogenizer in RNA lysis buffer containing 1% β-mercaptoethanol and RNA was isolated following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... following standard protocols supplied in the Pierce Mouse IgG1 Fab Preparation Kit (Thermo Fisher). Complexes of refolded MAGEA4 pMHC bound to 2M2 Fab were isolated by mixing the two components and separating the complex by SEC using a Superdex 200 Increase column ...
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bioRxiv - Microbiology 2022Quote: ... and Dynabeads™ Untouched™ Mouse CD4 Cells Kit were purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Systems Biology 2022Quote: ... To enrich for lineage negative (Lin-) cells Dynabeads Untouched Mouse CD4 Cells kit (Invitrogen) were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched using Dynabeads Untouched Mouse CD8 Cells Kit (Invitrogen, #11417D) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... and brachial lymph nodes with the Dynabeads Mouse DC Enrichment Kit (cat# 11429D, Invitrogen) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Naive CD4+ T cells were isolated via Dynabeads untouched mouse CD4 isolation kit (ThermoFisher) according to kit specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from total mouse splenocytes using PureLink Genomic DNA kit (Invitrogen). 500ng of DNA was subsequently used for library preparation using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® following manufacturer’s recommendations ...