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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Cells were then treated with APC-conjugated F(ab’)2-goat anti-mouse secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... at room temperature for 2 hours and incubated with donkey anti-mouse-Alexa 488 (Invitrogen Molecular Probes) at 4 µg/ml for 45 min at room temperature in the dark ...
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bioRxiv - Genetics 2021Quote: ... at room temperature for 2 hours and incubated with donkey anti-mouse-Alexa 488 (Invitrogen Molecular Probes) at 4 µg/ml for 45 min at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse SARS-CoV-2-spike (GTX, 1:100) and anti-rabbit hACE2 (Thermo Fisher,1:100) were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores ...
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bioRxiv - Plant Biology 2020Quote: ... 4 hour incubation in MTSB containing 2 % BSA and 0.5 % Alexa-conjugated anti-mouse secondary antibody (Invitrogen), 9 washes for 15 minutes in MTSB ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μg/ml Alexa Fluor 488-conjugated goat anti-mouse IgG for two hours (Thermo Fisher Scientific Cat# A-11029 ...
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bioRxiv - Genomics 2022Quote: ... manufacturer details in Supplementary Table 2) and the labeled cells subsequently incubated with Mouse Depletion Dynabeads (Invitrogen). Purification of lineage-negative cells was performed using a DynaMag-15 magnet (Invitrogen) ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... which were either 2 µg/ml donkey anti-mouse Alexa Fluor 488 (Cat#A21202, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 488 (Cat#A21206 ...
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bioRxiv - Microbiology 2021Quote: Mouse tissue from SARS-CoV-2 infected mice was homogenized using glass beads in TRIzol Reagent (Invitrogen). Equal volume of 100% EtOH was mixed with the TRIzol homogenate and processed using Direct-Zol RNA MiniPrep Kit (Zymo ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained as follows: 1-blocking with 2 μg/mL FcBlock (anti mouse CD16/CD32, Invitrogen) for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μg/ml Alexa Fluor 647-conjugated donkey anti-mouse IgG for two hours (Thermo Fisher Scientific Cat# A-31571 ...
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bioRxiv - Microbiology 2020Quote: ... at a 2 μg/mL concentration followed by goat-anti-mouse-HRP antibody (Thermo Fisher, Cat # 32230) at a 0.5 μg/mL concentration ...
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bioRxiv - Immunology 2021Quote: ... and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific)) for 20 min before staining for specific surface markers with fluorescence-conjugated antibodies for 25 min at 4°C (Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Synthetic Biology 2023Quote: ... were coated with biotin-conjugated mouse anti-HA monoclonal antibody (10 μg ml−1; 2-2.2.14, ThermoFisher) in ELISA buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... secondary antibody F(ab’)2-goat anti-mouse IgG (H+L) HRP-conjugate (1:5,000 dilution, Invitrogen), and secondary antibody goat anti-rabbit IgG (H+L)-HRP-conjugate (1:10,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized using the mouse T regulatory cell staining kit (Invitrogen, USA) and stained with anti-FOXP3-PE-Cy5 (Clone ...
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bioRxiv - Developmental Biology 2021Quote: ... Mitochondria from mouse ESCs were isolated using Mitochondria isolation kit (89874, Thermo Scientific).
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bioRxiv - Physiology 2022Quote: ... mouse lungs or i-EndoMT cells were isolated using the mirVana kit (Invitrogen). PPARγ ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: Extraction with the Ribominus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific) was performed according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was precipitated overnight at −20°C by the addition of 1 volume of isopropanol and 2 μL of GlycoBlue (15 mg/mL, Invitrogen). RNA was pelleted by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Genomics 2022Quote: ... plugs were melted two minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific) for 45 minutes at 43°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated (2 hr at 21 °C with 1100 RPM shaking) with AcTEV protease (20 units in 100 μL, Invitrogen) to digest the protease site between the HaloTag and baits.
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bioRxiv - Molecular Biology 2019Quote: ... TCam-2 cells were grown in 37°C and 5% CO2 in Roswell Park Memorial Institute (RPMI, Life Technologies 61870044) 1640 medium supplemented with 10% FBS (GE Healthcare HyClone SH30071 ...
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bioRxiv - Molecular Biology 2019Quote: ... Reactions were started by the addition of ATP (0.5 mM final) and quenched after 2 min at 30 °C with EDTA (100 mM) and 4X LDS buffer (Invitrogen). Reactions were run on 4-12% Bis-Tris gel in MOPS buffer (ThermoFisher Scientific ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: Macaque PBMCs (2×106cells/well) were cultured for 44 hr (37°C, 5% CO2) in 500 μl of IMDM (ThermoFisher) supplemented with 10% FCS and 1% penicillin/streptomycin in the presence or absence of 20 μg/ml of rhMOG ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... After that cells were incubated for 30 minutes in live-cell imaging solution containing 2 μM C-11 BODIPY (Invitrogen). Cells were then washed with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were used at 1:200 dilution in PBT for 2 hours at room temperature or at 4 °C overnight (Goat anti-Chicken Alexa 488, ThermoFisher Scientific cat#A11039 ...
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bioRxiv - Microbiology 2020Quote: ... The radiolabeled probe was heated to 100°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Ambion). Blots were incubated with 10 mL of the resuspended probe overnight at 42°C.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Genomics 2022Quote: ... The cells were incubated at 37 °C for 24 hours and harvested in 2 ml of TRIzol™ (Invitrogen™). RNA was extracted following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then pre-hybridized for 2 hr at 50°C in SCC buffer containing 50% deionized formamide and 40 μg/mL salmon DNA (Thermofisher) and then incubated with sense or antisense probes at 75°C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT) at 37°C for 2 h followed by incubation with 0.75 U UDG (EN0361, Thermo Fisher Scientific) in 1x UDG buffer at 37°C for 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... 721.221 cell lines were kept in a humidified incubator at 37°C with 5% CO2 and cultured in complete RPMI1640 medium supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher) ...
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bioRxiv - Genomics 2022Quote: ... MDA-MB-231 and HEK-293T cell RNAs (5 μg) were incubated with 2 U TURBO DNase for 30 min at 37 °C (ThermoFisher). After DNA digestion ...
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bioRxiv - Immunology 2022Quote: ... and reduced at 37°C with 1:125 dilution of Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (ThermoFisher, Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2, ATCC) were maintained in T-75 flasks (Falcon) at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Immunology 2020Quote: ... CAR T cells were incubated for 30 min at 37°C with either 1μM fura-2 AM or fluo-4 AM (Life Technologies). T cells were then washed in HBSS and resuspended in TexMACS complete medium with 3% AB serum ...
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bioRxiv - Molecular Biology 2020Quote: ... The Cas12a/gRNA trans-cleavage fluorescence reaction was incubated at 37°C for 2 hours and its fluorescence was collected every 1.25 minutes by fluorescence detector (Applied Biosystems 7500 Real-Time PCR System in this study ...