Labshake search
Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
-
bioRxiv - Molecular Biology 2023Quote: ... Stock probes were diluted 1:1000 in RNA FISH hybridization buffer preheated to 42°C (15% deionized formamide, 10% dextran sulfate, 2× SSC from Ambion, 0.02% BSA ...
-
bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/ml) (Invitrogen), resuspended in DMEM (Lonza ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... Vials of frozen PBMCs were thawed in a 37 °C water bath for approximately 2 minutes prior to resuspension and washed in warmed media composed of RPMI-1640 (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
-
bioRxiv - Microbiology 2023Quote: Primers were pre-annealed to the RNA by heating for 2 minutes at 95 °C prior to an RT step carried out using SuperScript III Reverse Transcriptase (Invitrogen) as described previously (15) ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated in a 5% CO2 environment at 38.5°C in 2 mL RPMI 1640 media (Gibco, UK) with 10% FBS (Gibco One-Shot FBS ...
-
bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
-
bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gradient was centrifuged at 200.000×g for 2 hours at 4°C in a S52-ST rotor (Thermo Scientific) and subsequently fractionated into 14 fractions using the BioComp Piston Gradient Fractionator device ...
-
bioRxiv - Immunology 2024Quote: ... were grown as a monolayer at 37°C with 5% CO2 in RPMI 1640 supplemented with 2 mM L-glutamine (31870-025, Thermofisher), 1 mM sodium pyruvate (11360-039 ...
-
bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
-
bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
-
bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
-
bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
-
bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
-
bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
-
bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Flag-Tdrd3-N and Flag-Tdrd3-C were synthesized with an mMESSAGE mMACHINE SP6 kit (Ambion), and the resulting mRNAs (2 μg ...
-
bioRxiv - Bioengineering 2019Quote: ... TADV-C or WT adult females using the Ambion mirVana mRNA Isolation Kit (ThermoFisher Scientific #AM1560). Following extraction ...
-
bioRxiv - Molecular Biology 2021Quote: ... 37°C) followed by reverse transcription using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Semi-quantitative polymerase chain reactions were performed and gene expression was normalized to β-actin ...
-
bioRxiv - Microbiology 2021Quote: ... Complimentary (c)DNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™) per manufacturer’s instructions and diluted 1:5 in nuclease-free water ...
-
bioRxiv - Immunology 2023Quote: ... In vitro transcription was performed at 34°C using a T7 MEGAscript T7 Transcription kit (Invitrogen). RNA was purified via lithium-chloride precipitation ...
-
bioRxiv - Neuroscience 2024Quote: ... Hybridization was done for 20 hours at +48 °C (AF-900454 GeneChip Hybridization Control Kit, Affymetrix). It was followed by the wash and stain protocol (AF-901910 FlashTagTM Biotin HSR RNA Labeling Kit ...
-
bioRxiv - Cancer Biology 2023Quote: FACS to detect definitive endoderm cells was performed using APC anti-c-KIT (Invitrogen, Cat# CD11705), PE anti-human CXCR4 (Biolegend ...
-
bioRxiv - Molecular Biology 2024Quote: ... and c-Myc using the CytoTune™-iPS 2.0 Sendai Reprogramming kit (Thermo Fisher Scientific, A16518) according to manufacturer’s protocol.
-
bioRxiv - Genetics 2021Quote: ... The solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) (Thermo Fisher, EH2IL2) was used to measure IL2 concentration from cell supernatant according to the manufacturer’s protocol(https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014639_EH2IL2_ELISA_Kit_PI.pdf) ...
-
bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 80 μL/well diluent SuperSignal Elisa Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) was added ...
-
bioRxiv - Immunology 2020Quote: ... Plates were developed using 1-Step Ultra TMB-ELISA (34028, Thermo Fisher) for one to two minutes after which the reaction was stopped with 1M H2SO4 ...
-
bioRxiv - Biophysics 2019Quote: ... 300 μL of SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) were added and the luminescence was measured using a Glomax 20/20 Luminometer (Promega ...
-
bioRxiv - Immunology 2019Quote: ... overnight at 4C on Nunc Maxisorp 96-well ELISA plates (Thermo Scientific). Wells were washed once with PBS and 1×105 CAR+ T cells were plated in 200uL CM and cultured for 24h ...
-
bioRxiv - Microbiology 2021Quote: PPS3 ELISAs were performed using 96-well Nunc Maxisorp plates (Thermofisher Scientific) coated with purified PPS3 (ATCC ...
-
bioRxiv - Biochemistry 2020Quote: ... and developed with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... Plates were developed using SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) and read at 425 nm ...
-
bioRxiv - Microbiology 2020Quote: ... ELISA was performed as follows: 96-well MaxiSorp plates (NUNC, Roskilde, Denmark) were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen ...
-
bioRxiv - Bioengineering 2022Quote: ... We coated ninety-six-well ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific) overnight with epitope-scaffolds in PBS buffer ...
-
bioRxiv - Immunology 2022Quote: ... ELISA was read by adding 100uL/well TMB substrate (Thermo Scientific, N301), allowing 10 minutes to develop in dark ...
-
bioRxiv - Microbiology 2022Quote: ... and then they were plated in 96-well ELISA MaxiSorp plates (Nunc) at 100 μl in each well ...
-
bioRxiv - Bioengineering 2022Quote: ... We coated ninety-six-well ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific) overnight with epitope-scaffolds in PBS buffer ...
-
bioRxiv - Immunology 2021Quote: ... NVX-CoV2373 Spike protein was coated on Maxisorp ELISA plate (Thermo Fisher), and then blocked with 5% BSA ...
-
bioRxiv - Neuroscience 2021Quote: The concentration of Aβ in TGCM was determined by ELISA (ThermoFisher Scientific) following the manufacturer’s instructions and was adjusted to 2000 pM Aβ 40/200 pM Aβ 42 prior to use ...
-
bioRxiv - Immunology 2022Quote: ... ELISA plates (Nunc MaxiSorp™ flat-bottom, Thermo Fisher Scientific, Waltham, MA) were coated with antigen (10ng/50μL ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 µg/ml TcGST were immobilised onto ELISA plates (Maxisorp, Thermofisher Scientific), free binding sites were blocked with Superblock (Thermofisher Scientific ...
-
bioRxiv - Pathology 2021Quote: ... sandwich enzyme-linked immunosorbent assays (ELISAs; Invitrogen, cat #s KHB0051 and KHB0041) were performed on brain homogenate (10% in PBS ...