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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from the thalamus of 2 GAERS (10 and 90 days) and 2 NEC (10 and 90 days) male rats using a MagMax Kit (Ambion) and full length cDNAs generated using SuperScriptII with oligo-dT priming (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Cetuximab and Avelumab F(ab’)2 fragments were generated using the Pierce F(ab’)2 preparation kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... fraction in MACS buffer (2% heat-inactivated FBS, 2% 0,1M EDTA in PBSO) using the CD8+ Dynabead isolation kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-alpha-Tubulin AA4.3-c (1:5000; DSHB)) were detected using HRP-conjugated secondary antibodies (1:10000; Thermo Fisher) and ECL Prime kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse Alexa Fluor 546 immunostaining (Thermo Fisher #A-11003 at 1:500 for 1 hr at 37 °C), and Hoechst 33342 staining (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were incubated with primary antibody for either 1 hour at room temperature or at 4°C overnight using the following dilutions: 1:1,000 mouse-α-HA 2.2.14 (Thermo Fisher 26183), 1:4,000 rabbit-α- PfClpP and 1:4,000 rabbit-α-PfClpR47 (kind gifts from Walid Houry) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: Mouse tissues were fixed in 4% paraformaldehyde at 4°C overnight and embedded in paraffin or Sandon Cryomatrix (Thermo Scientific). Standard histology techniques were used ...
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bioRxiv - Biophysics 2019Quote: ... Then they were incubated for 45 min at 37°C with 1:400 anti-mouse AF647 antibody (Life Technologies, A21237) in PBS + 1% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... Labeled DNA (500 ng) was precipitated during 30 min incubation at −20°C after adding 5 μl of mouse Cot-1 DNA (Invitrogen), 0.5 volume of 7.5 M ammonium acetate and 2.5 volumes of cold 100% ethanol ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C and then with 1:250 goat anti-mouse Alexa Fluor 488 (A11008; Life Technologies, Waltham, MA) and 1:250 goat anti-rabbit Alexa Fluor 647 (A21244 ...
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bioRxiv - Cell Biology 2021Quote: ... was applied at 1:7500 dilution and incubated overnight at 4°C with horizontal platform shaking (62 RPM) followed by secondary goat anti-mouse-HRP antibody (A-10668; Invitrogen) incubation with three 5-minute Tris-Buffered Saline 0.1% Tween 20 (TBST ...
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bioRxiv - Plant Biology 2021Quote: ... a mouse anti-FLAG primary monoclonal antibody was used (anti-DYKDDDDK FG4R, 1 μg/mL overnight at 4 °C, Invitrogen), and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were incubated with primary antibodies at 37 °C with following dilutions: anti-SPARC mouse at 1:800 (33-5500, Invitrogen), anti-SIRPα rabbit at 1:25 (13379 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with either mouse anti-mitochondria antibody (Thermo Fisher Scientific, 1:200, # MA5-12017); mouse anti-nuclei antibody (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_10541045) for 3 days at 4°C followed by 2h in 1:800 anti-mouse FluoroNanogold (Life Technologies, A24920) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... were washed three times in sterile PBS and opsonized overnight at 4°C in 3 mg/mL mouse IgG (Invitrogen). To remove excess antibody ...
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bioRxiv - Molecular Biology 2021Quote: For tracking experiments cells were labelled with 5 nM JF549-HaloTag ligand for 15-30 minutes at 37 °C in mouse ES imaging medium (FluoroBrite DMEM (ThermoFisher, 10 % FCS supplemented with nonessential amino acids ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies were used at 1/200 concentration overnight at 4°C and secondary AF488-conjugated anti-mouse Ig (Life Technologies) at 1/500 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Biochemistry 2020Quote: ... in the same buffer at 4 °C overnight followed by incubation with Alexa Fluor 594 anti-mouse IgG Ab (1:1000, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated overnight at 4°C in blocking-permeabilizing solution containing antibodies anti-PAX6 (mouse, 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... at 1:500 dilution for 1 h at 4°C followed by donkey anti-mouse conjugated to Cy3 and donkey anti-rabbit conjugated to 488 (ThermoFisher) secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... at 37°C and 5% CO2 in mitotically arrested Mouse Embryonic Fibroblasts (MEFs) conditioned media (DMEM/F12 (Life Technologies, #11330057), 20% Knock-out Serum Replacement (KSR ...
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bioRxiv - Immunology 2019Quote: ... then incubated for 90 min at 37°C with goat anti-mouse and Rat antibodies conjugated to AlexaFluor-488 or 568 and DAPI (Invitrogen). All microscopy was performed using Nikon Structured illumination microscopy (N-SIM ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Fixed cells were incubated with anti-MyHC antibodies (MF20) overnight at 4°C and then incubated with anti-mouse secondary antibodies labeled with Alexa fluor 594 (Invitrogen) for 1 hour at room temperature and counterstained with DAPI ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Cell Biology 2022Quote: Cortices isolated from E17 mouse embryos were dissected and dissociated with trypsin at 37°C (Thermo Fisher Scientific, 15050-065). Cells were plated in MEM/FBS on coverslips previously coated with 1mg/ml poly-L-lysine in Borate Buffer for 72 hours followed by three 1 hour washes with sterile water ...
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bioRxiv - Evolutionary Biology 2022Quote: We synthesized the Bowhead whale CDKN2CRTG protein with mouse codon usage (GeneScript) and directly cloned the synthesized gene into the pcDNA3.1-C-eGFP vector (Life Technologies), which added a C-terminal eGFP tag ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... the cells were incubated for 90 min at 37 °C with AlexaFluor-488 conjugated goat anti-mouse and AlexaFluor-568 goat anti-rat antibodies and DAPI (Invitrogen). Fluorescence microscopy was performed using a Nikon Structured illumination microscope (N-SIM ...
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer, Ref# A21202, Invitrogen) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the tissues were treated overnight at 4°C with a secondary polyclonal goat anti-mouse Alexa Fluor 488 antibody (A11001, Invitrogen) diluted 1:200 in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Individual blastocysts were then deposited into wells containing Mitomycin C-inactivated mouse embryonic fibroblasts as feeder cells and cultured in KnockOut DMEM (Gibco) supplemented with 20% Knockout Serum Replacement (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... and the OLLAS tag was detected using the rat mAb anti-OLLAS.72 The c-Myc epitope was detected with mouse anti-Myc (mAb 9E10) or rabbit polyclonal antibody (pAb) (#PA1981; Invitrogen). Toxoplasma-specific antibodies include rabbit pAb anti-IMC673 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C: IL-15Rα-PE (clone DNT15Ra; Invitrogen), CD40-BV421 (clone 3/23 ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed 3X in 0.1% PBST and incubated in goat anti-mouse Alexa Flour 647 antibody overnight at 4°C (1:300, ThermoFisher A32728). Images were acquired using a Zeiss 880 Laser Scanning Confocal inverted microscope with a Plan-Apochromat 63x/1.4 oil DIC M27 lens ...
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bioRxiv - Immunology 2023Quote: ... were either cocultured with irradiated (20Gy) BALB/c mice BMDCs (5 × 104) or activated using mouse T-activator CD3/CD28 Dynabeads (Gibco) at a 1-to-1 bead-to-cell ratio ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2023Quote: NIH/3T3 embryonic mouse fibroblast cells (ATCC, CRL-1658) were cultured at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... Primary bone marrow-derived macrophages (BMDM) were differentiated from male Balb/C mouse femur and tibia bone marrow in 20% L929 medium (Gibco DMEM medium ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed overnight at 4°C with 100 μL of BSA-blocked Pan Mouse IgG Dynabeads (Invitrogen, 110.41). The precipitated material was ChIP-exo treated ...
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bioRxiv - Genetics 2023Quote: ... Samples were incubated for 2 hours at 4°C with rotation before adding 50 µL sheep anti-mouse IgG dynabeads washed in IPP buffer (Invitrogen). The unbound fraction was retained ...
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bioRxiv - Immunology 2024Quote: ... the membranes were probed overnight at 4°C with anti-mouse CASP-1 (Cat# 14-9832-82; Thermo-Fisher Scientific) or anti-mouse mature IL-1β (Cat# 63124S ...
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bioRxiv - Developmental Biology 2019Quote: ... Dorothy genotyping was performed using the TaqMan™ Sample-to-SNP™ Kit using proprietary primers designed by the vendor targeting Nubp2:c:626T>A or Zbtb12:c:1061C>A (Thermo Fisher Scientific, Waltham, MA, USA). The Nubp2tm1a (Nubp2null ...
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bioRxiv - Cancer Biology 2021Quote: ... mixed with 2 μL of anti-mouse CD16/CD32 antibody (cat. #14-0161-85, Thermo-Fisher Scientific), and incubated at 4°C for 20 min to block nonspecific IgG binding ...