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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Cytokines in mouse lung homogenates were measured using a custom ProcartaPlexTM Mouse Mix & Match Panels kit (Invitrogen, Waltham, MA, USA) on the Bio-Plex 200 (Bio-Rad Laboratories Ltd.).
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... MyoD staining was performed with the Zenon Mouse IgG Labeling Kit (Invitrogen) according to the manufacturer’s instructions ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... followed by treatment with Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen, 11415D) to isolate the CD4+ T cells ...
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bioRxiv - Genomics 2022Quote: ... globin RNA was reduced using the Ambion GLOBINclear-mouse/rat kit (Thermofisher). RNA yields were quantified using the NanoDrop ND1000 spectrophotometer (Thermofisher ...
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bioRxiv - Immunology 2024Quote: Splenic DC were enriched using the Dynabeads Mouse DC Enrichment kit (Invitrogen) and sorted on a BD FACS Melody to obtain cDC1 (B220−CD64−CD3−CD19−NK1.1−CD11chiMHCII+XCR1+ ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was denatured at 65°C for 2 min and then added to 40 U RNAse inhibitors (RNaseOUT-Invitrogen) and 400 U M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and (3) 110,000 × g for 2 hours at 4°C with a Sorvall Discovery SE ultracentrifuge (Thermo Fisher Scientific) with an AH-650 rotor (k factor ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Neuroscience 2020Quote: ... 4% paraformaldehyde in 0.1M cacodylate buffer with 3mM CaCl2) warmed to 37°C with 2 mL of Neurobasal-A medium (Gibco,) containing 2% B-27 (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... The marrow was flushed out of the bones via 2 - 5 mL 4 °C DPBS (Dulbecco’s Phosphate-Buffered Saline; Cat. No. 21-031-CV; Gibco) injection ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Microbiology 2021Quote: ... After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco) supplemented with 5% New Born Calf Serum (NBCS ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae strains were grown overnight at 37°C to stationary phase in LB-Lennox broth (Fisher Scientific, BP1427-2) with constant agitation ...
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bioRxiv - Cell Biology 2021Quote: ... under 5% CO2 at 37°C in humidifying conditions and passaged every 2-3 days using 0.25% Trypsin-EDTA (25200056, Gibco). Cells were routinely tested for mycoplasma ...
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bioRxiv - Cancer Biology 2022Quote: ... Mesenteries were then stored for up to 48hrs at 4°C into PBS with 2% Antibiotic-Antimycotic solution (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed twice at room temperature and further incubated with the CCF2/AM dye for 2 h at 37°C according to the manufacturer’s instructions (Invitrogen). Cells were subsequently fixed with 1% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2022Quote: ... 1.5×106 CD45.1 cells were incubated with 2×106 Balb/c splenocytes in the presence of 1 mg/mL GolgiStop (Invitrogen) for 4-5 h at 37 C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Homogenised leaf tissues were centrifuged at 18,000 g 10 min 4 °C and 2 μL supernatant mixed with 48 μL Bradford reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were polymerized for 2 h at 37°C and then digested with 8 U/ml Proteinase K (Invitrogen) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific), 10% methanol ...
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bioRxiv - Immunology 2022Quote: ... Cells then were washed twice with FACS buffer and stained for 5 minutes at 4°C with 0.5 mg/mL of 40,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher). PE and DAPI staining were measured with an iQue Screener Plus flow cytometer (Intellicyt ...
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bioRxiv - Neuroscience 2023Quote: ... and concentrated by centrifugation at 85,000x g for 2 hours at 4°C in a Sorvall WX 100 Ultra Ultracentrifuge (ThermoFisher). The supernatant was discarded and viral pellet resuspended in a volume of PBS containing calcium and magnesium (#14090-055 ...
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bioRxiv - Plant Biology 2022Quote: ... then incubated in 0.2x SSC for 2 h at 55°C and treated with RNase A (Thermo Scientific #EN0531) at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Equivalent amounts of protein (∼2 mg) were used for immunoprecipitation with Pierce anti-c-Myc Agarose (Thermo Scientific, 20169), anti-Flag Affinity Gel (Bimake ...
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bioRxiv - Cell Biology 2023Quote: ... transfected HEK-293 cells plated on poly-lysine-coated glass coverslips were loaded for 20 min at 37 °C in standard bath solution containing membrane-permeable Fura-2 acetoxymethylester (1.5 ng/µl; Invitrogen) and pluronic acid (0,025%) ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2023Quote: ... Corning® Bio-Coat® Matrigel® Invasion Chambers with 8.0 μm PET membranes were rehydrated for 2 h at 37 °C using NB-A medium with only 100 U/ml Penicillin 100µg/mL Streptomycin (Gibco) added ...
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bioRxiv - Cell Biology 2023Quote: ... for 2–5 h at 37°C followed by a 5 min incubation in TrypLE express (Thermo Fisher Scientific) to generate small clumps of corneal endothelial cells ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were stored in 2% PFA at 4°C prior to analysis using an Attune NxT Flow Cytometer (Invitrogen) in the Flow Cytometry Core Facility of the Faculty of Medicine & Dentistry at the University of Alberta ...
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bioRxiv - Genetics 2023Quote: ... The root (∼60 mg) was incubated for 2 h at 56°C with 950 μl 0.5 M EDTA pH 8.0 (Thermo Fisher), 50 μl 1% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBS/5% FBS at 37°C for 10 min or 2 μM H2-DCFDA (Thermo Fisher Scientific, C6827) in PBS/5% FBS at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Samples were spun for 2 hours at 36,000 rpm (∼110,000 x g) at 18°C (AH-650 swinging bucket rotor Thermo Scientific), then 250 µL fractions were manually collected ...
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bioRxiv - Synthetic Biology 2023Quote: WT HeLa (ATCC CCL-2) were cultured at 37 °C with 5% CO2 in flasks with Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 1 mM L-glutamine (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then kept at 28.5°C with fresh E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics). Around 3-4 hours after heat-shock ...
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bioRxiv - Cell Biology 2024Quote: HeLa (ATCC CCL-2) cells were grown at 37 °C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... TGF-β1 and monocyte chemotactic protein-1 (MCP-1) was measured using the ELISA kit following the manufacturer’s protocol (eBioscience Affymetrix, Santa Clara,CA, United States). The levels of Muc5ac and Muc5b in BALF were also measured according to Lu et al ...
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bioRxiv - Immunology 2021Quote: Supernatants from 48-hour rested and stimulated iNKT cells and TCONV were assayed for cytokine levels of IFN-γ using human ELISA kit (Invitrogen cat #88-7316) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: TNF-α was quantified in cell culture supernatants of infected cells by Ready-SET-Go! ELISA kits (Thermo Fisher Scientific, Cat. 88-7324-77) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... ratio and pERK:ERK (pT202/pY204, pT185/pY187) ratio was done using the AKT (Total/Phospho) InstantOne ELISA Kit (Thermo Fisher Scientific, 85-86046-11) for pAKT:AKT ...
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bioRxiv - Immunology 2023Quote: ... 10 μgs of protein were loaded into manually coated IL-22 96-well High Affinity plates and IL-22 levels were determined by IL-22 ELISA kit (ThermoFisher-Scientific #88-7422-22) following manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested and cytokine concentrations measured by ELISA (Thermo Fisher) and cells were stained and analysed using flow cytometry as described below.
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested and cytokine concentrations measured by ELISA (Thermo Fisher) and cells were stained and analysed using flow cytometry as described below.
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bioRxiv - Immunology 2022Quote: ... 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific: 34029) was added for colorimetric detection ...