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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: Systemic inflammation in plasma was assessed by measuring cytokine levels using commercially available quantitative sandwich ELISA kits for interleukin 1 beta (IL-1β) (Invitrogen, USA, Cat no.:88-7013-88), and tumor necrosis factor α (TNF-α ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
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bioRxiv - Physiology 2023Quote: Thirty-six cytokines and chemokines were measured in young and old mouse plasma by luminex multiplex technique using a Cytokine & Chemokine 36-Plex Mouse ProcartaPlex™ Panel 1A kit (Thermo Fisher Scientific). The luminex multiplex assay was performed using undiluted plasma samples following the manufactures instruction.
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Systems Biology 2019Quote: Liver endothelial cells were incubated for 1 h at 37°C with 1 µM Fura-2 AM (Thermo Fisher Scientific, USA) and 0.1% pluronic acid in Standard Bath Solution (SBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were resuspended in 10 ml phosphate buffered saline (PBS) and crosslinked at 25°C with 2 mM DSG (Thermo Scientific) for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... RNA was eluted in 50 μl of RNase-free molecular biology grade water and DNA was removed by incubation with 1 μl TURBO DNase (2 U/μl) for 30 min at 37 °C according to the manufacturer’s instructions (Ambion via ThermoFisher Scientific, Germany). RNA samples were analyzed for quantity and integrity with a Qubit 4 Fluorometer (Invitrogen via ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... The remaining pieces of tissues were further digested at 37°C with shaking at 180 rpm for 45 min in 2% FCS in RPMI (Life Technologies), containing 1 mg/ml collagenase Type 1 (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... rRNA depleted RNA was fragmented by first heating the sample to 95°C for 2 min and adding RNA fragmentation buffer (1x, Thermo Fisher) for 30 seconds at 95°C and quenched by addition of RNA fragmentation stop buffer (ThermoFisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.2% SDS) at 50°C for 60 min on a Thermomixer (1200 rpm) followed by extraction with acid phenol (ThermoFisher, AM9712) and precipitation with 1:1 isopropanol:ethanol ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Cell Biology 2019Quote: Heat-killed bacteria were stained at 37°C with gentle shaking and protected from light for 30 min either with 2 µg/ml DyLight™650 (ThermoFisher) or 5 μM Oregon Green 488-X succinimidyl ester (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Plant Biology 2020Quote: ... and a final extension of 5 min at 72°C. The target band (approx. 100 bp) was extracted from a 2% E-Gel (Thermo Fisher). For multiplex sequencing ...
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bioRxiv - Genetics 2021Quote: ... worms grown at 25°C were incubated 48 hours prior to imaging with 2 μM LysoTracker Red DND-99 (Life Technologies). Worms were imaged as day 4 adults as described above ...
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bioRxiv - Pathology 2021Quote: ... Samples were incubated at 37°C for 30 min followed by DNAse inactivation by adding 2 μL of DNAse Inactivation Reagent (Ambion®). After 5 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was removed from the antibody by boiling at 100 °C for 5 min in 2 X LDS Sample buffer (Life Technologies). An equal volume of each sample and its corresponding input was loaded onto a gel for electrophoresis and western blot analysis.
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bioRxiv - Cell Biology 2021Quote: ... GFP-associated or immunoprecipitated complexes were eluted (30 μl) at pH 2 for 5 min at 25°C and neutralized according to the manufacturer’s instructions (88805; Thermo Fisher Scientific). Samples were then reduced at 70°C for 5 min by dilution in 5x sample buffer (125 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... isolated HCs before seeding were resuspended at 2×106/ml for 30 min at 37°C in high-glucose DMEM (11965-084, Invitrogen, USA) supplemented with 10% FBS (17L624 ...
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bioRxiv - Developmental Biology 2022Quote: ... Single cell dissociation of ALI cultures included a 2 min exposure to 0.25% Trypsin at 37°C (Gibco, Cat#25200-056), careful aspiration of Trypsin ...
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bioRxiv - Physiology 2022Quote: ... The corpus was incubated in 50% betaisodona/PBS (Mundipharma GmbH) for 30 min at 4°C before being washed in different solutions for 2 min each: PBS (ThermoFisher Scientific), 0.1% octenidin in ddH2O (Serva Electrophoresis) ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated at 37 °C and images were taken manually every 1 h for 24 h using Evos FL Auto 2 inverted microscopy (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then incubated in a water bath pre-adjusted to 37°C for 45 min in the dark with 2 µg/ml of Hoechst 33324 (Invitrogen, H3570) diluted in FACS buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 µL lysate was denatured for 2 min at 85 °C in 9.6 µL Novex Tricine SDS Sample Buffer (2X) (Thermo Fisher Scientific) and 1.4 µL DTT (500 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and incubation at 37°C for 1 hr and 2) 1 hour incubation at 50°C following addition of 15μl Proteinase K (20 mg/ml, Thermo Scientific™) and 30μl Proteinase K digestion buffer (0.1M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were enzymatically dissociated at 37°C for 2 × 20min in the HBSS solution supplemented with calcium (CaCl2 5 mM) and containing collagenase type II (Gibco, France) and dispase (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... Transfected cells were studied in PBS at 37°C for 2 h maximum in an Attofluor cell chamber (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Molecular Biology 2019Quote: ... the aqueous layer was removed to a new tube and precipitated overnight at −20°C with 2 ul Glycoblue (ThermoFisher #AM9516) and 900 ul of 100% ethanol ...
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bioRxiv - Immunology 2019Quote: ... and a final 10min 68°C extension) from 2-3μl of cDNA in 25μl of Accuprime Pfx supermix (Invitrogen, Thermo Fisher Scientific) and 0.5μl of the VH ...
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bioRxiv - Genomics 2019Quote: ... 5 μL of lysate was denatured for 2 minutes at 85 °C in 9.6 μL Novex Tricine SDS Sample Buffer (2X) (Thermo Fisher Scientific) and 1.4 μL DTT (500 mM) ...
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bioRxiv - Systems Biology 2020Quote: Similar to our recent report,16 1 mg nuclear protein extracted from Raw 264.7 macrophage cells was incubated overnight at 4°C with 2 nmole UNC0965 pre-coupled to 50 μl neutravidin-agarose (Thermo Fisher), and washed three times with 1 ml lysis buffer to remove non-specific bound proteins ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
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bioRxiv - Plant Biology 2020Quote: ... 15N-labeled seedlings were then dried for 2-3 days at 65°C and further analyzed by Thermo Finnigan Delta plus XP IRMS (ThermoFisher Scientific). For primary root length determination ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... 5×106 cells/ml PBMC were incubated at 37°C for 18 h or 2×106 cells/ml PBMC for 5 days in RPMI 1640 GlutaMAX medium (Life Technologies/Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2021Quote: ... 0.1 pmol of 32P-5’-end-labelled RNA were subjected to RNase T1 or Pb2+ probing at 30°C in the presence of 2 μg carrier yeast tRNA (Invitrogen #AM7119) (Supplementary Fig ...