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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Beads were then washed with ice-cold lysis buffer 3 times for 5 minutes and proteins were recovered by boiling in denaturing loading buffer (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatants containing both the cytosolic and glycosomal proteins were analysed by BN-PAGE on a precast (3–12%) Bis-Tris polyacrylamide gel (Invitrogen) according to standard methods.
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bioRxiv - Genomics 2021Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... An equivalent to 100 mg of mitochondrial proteins were loaded and separated on 3–12% (w/v) polyacrylamide NativePAGE Bis/Tris gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were separated on a NativePAGE Novex 3%–12% Bis-Tris protein gel system (Thermo Fisher Scientific, catalog BN1003BOX) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The RNA-protein complexes were then subjected to 3’ adapter ligation and reverse transcription with SuperScript III Reverse Transcriptase (Invitrogen). The cDNA was purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... 5×106 cell equivalents were loaded into wells on a 3-12% Bis-Tris gradient gel with unstained NativePage protein standards in the first well (ThermoFisher) and run according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Systems Biology 2023Quote: ... From this mixture 45 µL were loaded with gel loader tips on the NativePAGE 3-12% Bis-Tris precast protein gels (Invitrogen). For replicate one and two of PFDN2 aliquots of 1 µL and replicate 1 of UXT an aliquot of 0.5 µL was taken away for absolute bait protein quantification ...
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bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
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bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3–12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
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bioRxiv - Biochemistry 2024Quote: Blue native PAGE analysis was carried out using NativePAGE 3-12% Bis-Tris 1.0 mm Mini Protein Gels (Thermo Fisher). Samples contained 10 μg of protein in NativePAGE Sample Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... then equal quantities of protein lysate (3-10 µg) were separated by SDS-PAGE using 4–12% gradient gels (Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Biochemistry 2023Quote: ... an equivalent quantity of SLO protein or the d3_m construct (ca. 3 ug) was immobilized onto a MaxiSorp plate (Thermo Scientific), which was subsequently incubated with either 0.4 ug nAb (followed by serial half-dilution) ...
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bioRxiv - Genomics 2023Quote: ... Samples were placed into separate PCR tubes containing 3 μl of lysis buffer with 0.3 mg/ml protein kinase K (Thermofisher, 26160). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 25 mins at 35,000 x g and the supernatant was adjusted to approximately 3 mg/ml protein (Nanodrop 2000 C, Thermofisher Scientific). To capture the SVs for content detection ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the cell and tissue lysates were determined using BCA protein assay kit (Pierce, Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE and proteins were transferred onto a nitrocellulose membrane (Biorad) ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations of whole-cell lysate fractions were then determined using the BCA Protein Assay Reagent kit (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as standard ...
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bioRxiv - Genetics 2022Quote: ... Subcellular protein fractionations were performed using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, Catalog no: 78840) according to the product manual ...
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bioRxiv - Immunology 2020Quote: ... The total protein concentration in each cell lysate was measured using Pierce BCA Protein Assay Kit (Thermofisher, Waltham, MA). The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Protein content of whole cell lysate was measured with the BCA protein assay (Pierce™, Thermo Scientific, Landsmeer, Netherlands) to quantify the protein content of the samples in order to equalize for input material for SDS page ...
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bioRxiv - Microbiology 2019Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as a standard ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentrations of the cell lysates were determined with a BCA protein assay kit (Thermo Fisher Scientific, USA). The cell lysates were pre-cleaned by an off-target control antibody and aliquoted ...
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bioRxiv - Systems Biology 2019Quote: ... The protein concentration in the cell lysate was quantified with a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, UK) using the provided albumin as standard ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate protein concentration was determined using Pierce BCA protein assay kit (Cat#23225; Lot no. QI223168; ThermoFisher Scientific). 50 μg of cell lysate was loaded onto a 4-12% gradient gel and ran at 165 V constant for 45-60 min in 1X MOPS-SDS buffer (NuPAGE ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were extracted from CEC or FFE cells in M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) using the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration in total cell lysates was determined by a BCA protein assay kit (Thermo Fisher Scientific, PI23227). Equal amounts of protein samples were loaded on 10% glycine-SDS-PAGE gel ...
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bioRxiv - Physiology 2023Quote: ... protein concentrations in detergent-soluble cell lysates were determined using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific), and proteins were subsequently analyzed by western blot ...
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bioRxiv - Genomics 2022Quote: Proteins were prepared from flash frozen cell pellets using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, Cat # 78503) following vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2023Quote: Protein concentrations in cell lysates were determined by Pierce BCA Protein Assay according to the manufacturer’s protocol (Thermo Scientific). Aliquots of cell lysates containing 50 µg total protein were snap frozen ...
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bioRxiv - Immunology 2023Quote: Cells were lysed in Laemmli buffer and the protein content quantified using EZQ™ protein quantification kit (Thermo Fisher). Equal protein amounts were loaded and separated on 4%– 15% pre-cast gels (BioRad ...
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bioRxiv - Bioengineering 2023Quote: ... Protein content in cell lysates and EVs was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Between 5 and 20 μg of total protein (kept consistent for individual experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The protein lysates were separated using Bolt 4–12% Bis-Tris Plus Gels (Burlington ...
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bioRxiv - Neuroscience 2024Quote: Total protein concentrations were measured in cell lysates using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates were prepared from cultured cells with modified Tissue Protein Extraction Reagent (TPER) (Life Technologies Corporation, Carlsbad, CA) and a cocktail of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was used to determine total cell protein using the BCA Protein Assay Kit (Thermo Fisher Scientific, Germany).
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bioRxiv - Cancer Biology 2024Quote: A protein assay was performed on 10 µL of cell lysate using Pierce BCA Protein Assay Kit (23225, Thermofisher) and following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in Mammalian Protein Extraction Reagent (MPER, Thermo Scientific), supplemented with protease inhibitor and phosphatase inhibitor (Thermo Scientific) ...