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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Pathology 2020Quote: ... It consisted of the DMEM medium and F12 in ratio 1:3 (Gibco Life Technologies), supplemented with 2.5 ml FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were washed 3 times and incubated with goat-anti-rabbit-Alexa594 (Invitrogen; 1:1000) plus goat-anti-mouse-Alexa488 (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... the B27 supplement in 3:1 media was replaced by N2 supplement (Gibco, #17502‒048) and retinoic acid was reduced to 5 µM.
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Zoology 2021Quote: ... and 3) an Alexa Fluor® 488-conjugated streptavidin (S11223, Thermo Fisher Scientific; 1:500) for 2 h at 28°C ...
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bioRxiv - Developmental Biology 2021Quote: ... the blastomeres were stained with 1 µM TO-PRO-3 iodide (Thermo Fisher Scientific, T3605) for 30 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... at 1:2000 and a nuclear counterstain (TO-PRO™-3 Iodide ThermoFisher Scientific T3605) at 1:2000 was performed for 2 hours before 3x 15 minute PBS-T washes and a final PBS-T wash overnight at 4°C while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed 3 times in PBS then incubated in 1:40 phalloidin (Thermo Fisher) in PBS for 20 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 10 nl of 1 mM FluoZin-3 (FZ3, 847 Da, Thermo Fisher Scientific, Cat. F24194), 100 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were rinsed 3 times with 1 mL of 1X PBS (Gibco, 10010-023), quenched in 1 mL of 0.1% sodium borohydride/PBS solution (Sigma ...
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bioRxiv - Genetics 2022Quote: ... TO-PRO-3 Iodide carbocyanine monomer nucleic acid stain (1:1000, Thermo Fisher, cat# T3605) was used to stain DNA ...
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bioRxiv - Plant Biology 2022Quote: ... in a ratio of 3:1 (FuGENE:DNA) and 65 μl of Opti-MEM (Thermo Fisher) was added ...
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bioRxiv - Microbiology 2022Quote: ... or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa) (Thermo Scientific, 26617) were used as a ladder ...
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bioRxiv - Neuroscience 2022Quote: ... pH=7.4) of mice (1-3 months) using a vibratome (Microm HM 650V, Thermo scientific).Slices were then transferred to a slice chamber with ACSF (in mM ...
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bioRxiv - Genomics 2023Quote: ... 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP) (dATP, Thermo Fisher R0141 ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the embryos were pre-cleared in 1% potassium hydroxide (KOH) (Fisher Scientific, 1310–58-3) solution for 1 hour and stained in 0.005% alizarin red (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The lungs were inflated with 1-3 ml 2% UltraPure Low Melting Point Agarose (Invitrogen). Lungs and livers were fixed overnight at 4°C on a shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070; Gibco).
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bioRxiv - Cell Biology 2022Quote: ... anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase; catalog number MA5-15738; Invitrogen; dilution 1:5000), anti-TDP-43 (catalog number 10782-2-AP ...
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bioRxiv - Microbiology 2023Quote: ... using lipofectamine at the proportion 1:3 (DNA / Lipofectamine) in Opti-MEM (Thermo Fisher Scientific), and after 4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used as primary antibodies: FFAR 3 (1: 100, #PA5-97745, Invitrogen) and GPR 43(1 ...
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bioRxiv - Bioengineering 2023Quote: ... at a DNA:PEI ratio of 1:3 (w/w) or Lipofectamine 2000 (Thermo Fisher Scientific) at a DNA:Lipofectamine ratio of 1:2 (w/v) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were immunostained with anti-beta-3 Tubulin (Invitrogen, cat. no. 2G10-TB3, 1:500) in 2.5% NDS overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 594-conjugated anti-mouse IgG (1:1000 in PBS/3% BSA (Invitrogen, A11032)) was used as secondary antibody and incubated for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... containing fungal cells at MOI 1:3 and 0.02 µg/µL propidium iodide (Fisher Scientific). Images were taken every 3 min for 2 hours on an Ultraview VoX spinning disk confocal microscope using Volocity software (Version 6.5.1).
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bioRxiv - Immunology 2023Quote: ... 3 μm tissue sections were stained with polyclonal goat anti-mouse CD8α (1:2000, Invitrogen) antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with protease inhibitors (1 mM PMSF, 5 μg/ml aprotinin and 5 μg/ml leupeptin, #78443, Thermo Fisher Scientific, Waltham, MA). Fifty ul of Dynabeads Protein A/Protein G (#10015D ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubating at 37°C in a 5% CO2 humidified incubator in DFGM supplemented with 5 ng mL−1 TGF-β1 (Thermo Fisher Scientific) and 100 μg mL−1 ascorbic acid ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were again washed 3x with 1 mL DPBS for 5 min each followed by nuclear labeling for 5 min with 1 mL 300 nM DAPI dissolved in DPBS (Thermo Fisher Scientific) and a 5-min DPBS wash ...
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bioRxiv - Immunology 2024Quote: Analysis of cytokines and chemokines from sera and lungs homogenates collected 5 dpi and 5 dpc were conducted using the ProcartaPlex TM Mouse Cytokine & Chemokine Convenience Panel 1 26-plex (Thermo Fisher Scientific) following the manufacturer’s specifications ...
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bioRxiv - Microbiology 2024Quote: ... each culture was diluted 1:1000 in BHI + 5% yeast and 10μl were streaked on a BHI + 5% yeast agar (Thermo Fisher Scientific, CM1136) plate ...
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bioRxiv - Cell Biology 2022Quote: ... seeded on t25 flasks at 37°C 5% CO2 in DMEM/F12 (1:1) (ThermoFisher Scientific, Cramlington, UK) with 10%FBS ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with 5% BSA (GenDEPOT, A0100) in 1% TBST (Fisher Scientific, BP2471-1, BP337-100) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37C and 5% CO2 either in 1:1 mix of DMEM GlutaMAX (Gibco, 31966047) and F-12 media (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng of each probe were precipitated with 5 μl of Cot-1 DNA (1 mg/ml) (Invitrogen) and resuspended in 15 μl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and pYK-JRCSF HIV-1 expression plasmid in a 5:1 mass ratio with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual.
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were washed 3 times in PBS followed by incubation for 1 hour at RT with AlexaFluor-conjugated secondary antibodies (1:400, Molecular Probes) and 0.15% DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed with washing buffer (0.2%Tween-20/10mg·L-1Heparin/ PBS) for 1 day with 3 times refresh and incubated with dye-conjugated secondary antibodies (1:500, A-21245, Thermo Fisher) in antibody incubation buffer for 1 week at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... were harvested and placed in 3:1 ethanol:glacial acetic acid fixative and then transferred to fresh 3:1 fixative containing 1% Polyvinyl Pyrrolidone (BP431-100, Fisher Scientific, USA). Emerging anthers from the flower buds were isolated and squashed under a 22 × 22 mm glass cover-slip to provide the best anthers for pachytene analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were dry-mounted on slides using Fluoromount (Sigma ...