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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... in a ratio of 1:3 with a supplement of H2O2 (30%, Certified ACS, Fisher Scientific) at 200 °C for 50 min in a microwave digestion system (Titan MPS ...
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bioRxiv - Microbiology 2021Quote: ... were mixed at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were allowed to adhere for 1-3 hours before media was changed to Neurobasal (Invitrogen) supplemented with 1x B27 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: RNA from 1-3 x 106 cells was extracted and purified with TRIzol reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2016) are presented in Table 1 using an Applied Quant Studio 3 (Applied Biosystems, CA, USA). For PKoV RNA and PAstV-1 RNA detection ...
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bioRxiv - Microbiology 2020Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 µg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... and the nuclei were stained with TO-PRO®-3 1:1000 (Thermo Fisher Scientific, USA) for 10–20 min ...
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bioRxiv - Microbiology 2021Quote: ... at a 1:3 ratio (mg DNA/mg PEI) mixed in Opti-MEM (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The sections were counterstained with TO-PRO™-3 (T3605, Invitrogen, OR, USA; dilution 1:1000) and examined using a confocal laser microscope (DMi8 ...
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bioRxiv - Genetics 2019Quote: ... except that 1-Bromo-3-chloropropane was used instead of chloroform and DNAse (TURBO DNase, Ambion) treatment was carried out ...
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bioRxiv - Neuroscience 2022Quote: ... psPAX2 and pMD2.G with a ratio of 4:3:1 in Opti-MEM (Gibco, 31985070) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and transfected with 1:3 ratio (RNA:Lipofectamine) using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Genomics 2022Quote: ... with 1 ml per 4 million cells of 3 mM disuccinimidyl glutarate (DSG) (ThermoFisher Scientific, 20593) for 40 min at room temperature and quenched by 0.4 M Glycine for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 3% sucrose) diluted 1:4 in Leibovitz’s L-15 medium without phenol red (Gibco, Waltham, MA) and an adjusted osmolality of 340 mOsm using 1 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... with PBS (3 times) and incubated with a secondary antibody (1:500, Molecular Probes AF488 #A11008) and Hoechst33342 (1:2000 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... 1 mM L-cysteine) with papain (3 U/mL) and DNAse I (150 U/μL, Invitrogen) for digestion during 25 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 µL of template cDNA using a QuantStudio 3 real-tame PCR system (Applied Biosystems). PP2AA3 was used for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... 3% agarose gels were prepared with a 1:20000 dilution of SYBRSafe DNA gel stain (Invitrogen). Once complete the PCR products were loaded onto the gel and the electrophoresis was run on a Powerpac Electrophoresis system (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed and incubated with a 1:1000 dilution of To-Pro-3 (ThermoFisher) in PBS for nuclear staining and then mounted using VectaShield antifade mounting medium (Vector Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% P/S at a cell density between 3 × 105 and 2.5 × 106 cells/ml (GIBCO). Cells were always handled and maintained under sterile conditions (tissue culture hood ...
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bioRxiv - Genetics 2023Quote: ... the nuclei were stained by applying 3 mL of 1:1000 diluted DAPI (Thermo Scientific, 62248) in 2×SSC in 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then mounted using culture medium supplemented with 1 μM TO-PRO-3 (Life Technologies) for nucleic acid staining ...
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bioRxiv - Microbiology 2023Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 μg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... zebrafish were incubated for 3 h in 1 µM LysoTracker™ Deep Red (Invitrogen™, L12492) in E3 (DMSO) ...
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bioRxiv - Cell Biology 2023Quote: PASMCs were stained with Hoechst 33342 dye (3 μL/ml of 1:100 dilution; 62249; Invitrogen) for 15-30 min at 37° C to visualize apoptotic cells ...
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bioRxiv - Immunology 2024Quote: ... and a 3:1 CD3/CD28 mouse T-activator Dynabead to Treg ratio (Thermo Fisher Scientific). On day 3 of expansion ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Microbiology 2020Quote: ... The wells were washed 3 times with 1 mL PBS 1X and covered with a mixture containing secondary goat anti-rabbit-Alexa488 antibodies (Invitrogen Molecular Probe 2mg.mL-1, 1/300e) and DAPI (1/100e ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... at 37 °C with 5 % CO2 with 1 % penicillin/streptomycin (PS, 5000 U.mL-1 and 5000 μg.mL-1respectively; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Biochemistry 2020Quote: SHSY5Y cells were obtained from the Duke University Cell Culture Facility and were maintained at 37 °C with 5% CO2 in DMEM:F12 (1:1; Gibco) supplemented with 10% FBS (Sigma Aldrich ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Plant Biology 2019Quote: ... Rh (10 µg L-1) and Ir (5 µg L-1) in 2% trace analysis grade (Fisher Scientific, UK) HNO3 ...
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bioRxiv - Physiology 2021Quote: ... Frozen tissues were mechanically homogenized with 1 stainless steel bead (5 mm) in 1 ml Trizol (Thermo Fisher Scientific) by shaking for 50 s at 30 Hz (TissueLyser ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 min) and resuspended in 1 ml PBS containing 8 µl UltraPure BSA (50mg ml−1; AM2616, ThermoFisher Scientific) and filtered over Flowmi 40µm cell strainers (VWR ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac ...
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody diluted in 5% NGS in PBS (β-catenin 1:200, ProteinTech, rhodamine-phalloidin 1:200, Molecular Probes) for 36 hours at 4°C ...