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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... R: 5′- AAAGCACCGACTCGGTGCCAC-3′) and used as a template for in vitro transcription using MEGAshortscript T7 kit (Ambion/Thermo Fisher Scientific AM1354). 2ng gRNA was injected together with 1.9nM Cas9 protein (New England Biolabs M0386T ...
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bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... Samples were then washed in PBS (3 x 5 minutes in PBS at room temperature) and mounted in ProLong Gold (Invitrogen, Rockford, IL). For STochastic Optical Reconstruction Microscopy (STORM) ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of peptide mixture was loaded onto an Acclaim µ-Precolumn enrichment column (0.5 mm x 3 mm, particle size 5 microns, Thermo Scientific, USA) at a flow rate of 10 µl/min for 5 min in isocratic mode with a 2% acetonitrile ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed 3×5 min in PBS and incubated for 2 h with an Alexa Fluor® Plus 555-conjugated secondary antibody (Invitrogen A32816) diluted in the blocking solution ...
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bioRxiv - Bioengineering 2023Quote: ... the sections were washed with PBS (3 times, 5 min each time) and mounted with ProLong™ Diamond Antifade Mountant with DAPI (Thermo Scientific). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Both Brca2+/+ and Brca2-/- cell lines were cultured under low oxygen conditions (3% O2, 5% CO2, 37°C) using DMEM (GIBCO; 41966-029) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... The Nitrocellulose paper was washed 3 times for 5 minutes in PBST and chemiluminescent substrate (SuperSignal West Duro Extended Duration Substrate Thermo Scientific #34075) was applied ...
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bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were again washed with PBS (3×5 mins) and then mounted using ProLong™ Diamond Antifade Mountant without DAPI (Thermo Fisher). The images were scanned using VS120 whole slide scanner (Olympus USA ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked for two hours at room temperature with 5% nonfat dry milk (Lab Scientific bioKEMIX Inc., Danvers, MA) and 3% bovine serum albumin (Thermo Fisher Scientific) in 1X Tris-Buffered Saline (Quality Biological ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and 100 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-gal) (Thermo Fisher Scientific). After incubation overnight at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... R-5’-3’ UGGUUCAUCAGCCUUUAACTT) and mock siRNA (General Biosystems, China) were transfected into mouse DCT cells by Lipofectamine™ 3000 (Invitrogen, L3000015) (20 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were separated on an Ultimate 3000 HPLC with loading on a 5 cm C18 trap column Acclaim PepMap 100 (3 µm particle size, 75 µm diameter; Thermo Scientific, 164946) followed by a 15 cm PepMap RSLC C18 EASY-Spray column (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and 1492R (5’-TACGGYTACCTTGTTACGACTT-3’) [29–31] with the Invitrogen Platinum II Taq Hot-Start DNA Polymerase kit (Cat. No. 14966001, Invitrogen, Waltham, MA). The 16S rRNA gene amplicons were sequenced by GENEWIZ (Azenta Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... Gateway entry clones of the purified 5’-flank and 3’-flank PCR fragments were generated as described by the manufacturer (Invitrogen, Carlsbad, CA). The hygromycin resistance cassette (hygB ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated product was amplified with a G-quadruplex-specific common primer (NGS-1290, 5’-GGAGGGGAGGGGAGAGGGG-3’) and an adaptor-specific common primer for 12 cycles with Phusion U (Thermo Fisher, #F555L). One-tenth of the amplification product was subjected to another round of amplification using Illumina P5/P7 primers ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified 5’ NotI-APEX2-XhoI 3’ fragments were digested and ligated into the vector pcDNA5/FRT/TO (Invitrogen, Thermo Fisher Scientific). Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified 5’ NotI-APEX2-XhoI 3’ fragments were digested and ligated into the vector pcDNA5/FRT/TO (Invitrogen, Thermo Fisher Scientific). Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... for 13 hours at 37 °C and 5% CO2 in the presence of Brefeldin A (3 µg/mL; ThermoFisher; Waltham, MA, USA) for the last 10 hours of stimulation ...
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bioRxiv - Genetics 2024Quote: ... flanked by 1,400 bp-long sequences homologous to the mouse Dysf locus flanking the murine Dysf exon 44 (5’ and 3’) were purchased as GeneArt synthetic fragments readily inserted into the pMX vector backbone (Thermo Fisher Scientific) (Supplementary Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... antibodies were diluted 1:500 in PBT with 1% DMSO and 1% BSA and applied together with TO-PRO-3 (T3605, Thermo Fisher, Waltham, MA) diluted 1:1000 over- night at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... and resuspended into 5 mL of warm PBS containing 5 μg mL−1 Hoechst 33342 (Thermo Fisher Scientific, Waltham, MA, USA) to stain live leukocytes.
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bioRxiv - Microbiology 2020Quote: Experiments to determine the sensitivity of the two RT-qPCR methods and nested PCR were completed using serial dilutions of each transcript (5*103 to 10−1 copies/5 µL) in a previously described RNA storage buffer containing RNA storage solution (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and SN25 FL (1–206, R59C) or truncation mutant (11–206, R59C) were labeled with 5×molar excess Tetramethylrhodamine-5-maleimide (TMR) (Molecular Probes) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2022Quote: ... washed once with cold acetone and then dissolved in Laemmli buffer (Tris 10 mM pH 7.5, EDTA 1 mM [Fluka, Buchs, Switzerland], β-mercaptoethanol 5%, SDS 5%, glycerol 10% [ThermoFisher Scientific]). Sonication and centrifugation were repeated as above to pellet and eliminate possibly remaining cell debris ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cells grown on poly-L-lysine–coated coverslips were incubated in 5% CO2 at 37°C for 1 h with 5 µM MitoTracker Red CMXRos (Molecular Probes) in DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently a copy of chromosome XV was removed by counter-selection against the URA3 gene by replica-plating on SD medium containing 1 mg/mL 5-fluoroorotic acid (5-FOA, ThermoFisher Scientific) from a 100 mg/mL stock in DMSO ...
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bioRxiv - Immunology 2023Quote: ... Spleens were mashed through a 70 μm filter with 5 mL DMEM with 5% FBS and 1% Penicillin/Streptomycin (Life Technologies). Before excising lungs ...
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bioRxiv - Biophysics 2023Quote: ... membranes were immediately trimmed and placed in either 5% non-fat milk or 5% BSA in 1X TBS supplemented with 1% Tween (Thermofisher #1706475) (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μM CsA and 1 μM Ca2+ green-5N (CaG5N, Thermo Scientific). CaG5N fluorescence was monitored (485 nm excitation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 6 h (42-48 h post stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked with 5% skim milk in 1 x PBS (Invitrogen) for 1.5 hours and incubated with the respective primary antibodies diluted in blocking buffer for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... slides were blocked in 5% normal goat serum (#01-620-1, ThermoFisher), incubated in primary antibody (Table S1 ...
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bioRxiv - Cell Biology 2019Quote: ... and passaged every 4-5 days with 1 mg/ml dispase (Gibco).
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bioRxiv - Genomics 2020Quote: ... 2 µl 5 M NaCl and 1 µl GlycoBlue co-precipitant (Invitrogen). Samples were vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Genetics 2019Quote: ... 1×10^5 cells were then lysed in RIPA buffer (ThermoFisher, 899000) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 5 µM JC-1 dye (T3168, Molecular Probes) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 ml of 1 mg/ml collagenase-IV (Thermo Fisher Scientific incorporated), 500 μl of filtered (0.2 μm ...