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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Microbiology 2024Quote: ... 12 μl of culture was transferred to 1.2% agarose pads prepared in A+B medium and supplemented with an additional 0.5 μg mL−1 FM 4–64 (Life Technologies). Excitation/emission filters were TRITC/CY5 for membranes and FITC/FITC for GFP.
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bioRxiv - Molecular Biology 2024Quote: ... This was then run on a NuPAGE 4 to 12% Bis-Tris gel with 1 x NuPAGE MES running buffer (Invitrogen). Samples were then run alongside 5 μL of Novex Sharp Pre-Stained Protein Standard ladder at 180 V for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Larvae were thoroughly washed in PBS-Tx and incubated overnight at +4°C in secondary antibody (1:400 goat anti-chicken Alexa488 A-11039, ThermoFisher) in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in the same solution overnight at 4 °C with the primary antibodies anti-GFP (1:1000, Invitrogen) and anti-phospho-S6 Ribosomal protein (Ser236/236 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Systems Biology 2024Quote: ... or 5 μg cleared lysate from cardiomyocytes were purified by a brief 1 cm SDS-PAGE run (Nu-PAGE minigel 4-12%, Invitrogen). After Coomassie staining ...
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bioRxiv - Molecular Biology 2024Quote: ASOs were removed from 4 µl of DMS-modified RNA in 10 µl containing 1 µl of TURBO DNase (Invitrogen) and 1X TURBO DNase Buffer (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... The fibroblasts were incubated with primary antibody overnight at 4°C and then stained with the secondary antibody (all 1:1000, Alexa Fluor; Invitrogen) for 1 h ...
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bioRxiv - Immunology 2024Quote: ... and THP-1 cells were incubated with GZMK (50 nM) or C1s (50 nM) for 1 h on ice at 4 °C in HBSS with Ca2+ and Mg2+ (Gibco) containing 10 mM HEPES and 0.5% BSA (Millipore Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... before incubation with DAPI (4’,6-diamidino-2-phenylindole, dilactate, 0.1 µg ml−1 final concentration in PB, Invitrogen D3571) in 0.1 M PB for 10–20 mins at RT ...
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bioRxiv - Neuroscience 2024Quote: ... pH = 7.6) followed by an overnight incubation at 4°C with 1:1500 anti-mouse Tau-5 antibody (Invitrogen #AHB0042) in superblock blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with the primary antibody Chicken-anti-GFP IgY (1:1000, Invitrogen, A10262) to label GCaMP6f-positive neurons ...
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bioRxiv - Physiology 2024Quote: ... Cells were then washed 3 times with PBS and incubated in the dark at room temperature for 4 hours with the secondary antibodies (1:400) donkey anti-mouse IgG conjugated to Alexa Fluor 564 and (A10040, Invitrogen) goat anti-rabbit IgG conjugated to Alexa Fluor 488 (SA510166 ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed and incubated on ice for 1 hour before loading onto Native Novex WedgeWell 4-20% Tris-Glycine gels in Tris-glycine native running buffer (Invitrogen). Gels were run for 45 min at 150 V at 4°C before detecting the HEX-label using a Typhoon FLA 9500 instrument (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated for 4 hr in blocking solution with secondary antibody goat anti-rabbit Alexa Fluor 647 (1:1000, catalog # A32733, Invitrogen). Sections were washed in PBS and mounted on slides in DAPI containing mounting medium (VECTASHIELD ...
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bioRxiv - Cancer Biology 2024Quote: ... MitoSOX Red dye (4 μM) was then added along with the Hoechst 33342 nuclear counterstain (1 μg/mL) (Thermo Scientific) in DPBS containing Ca 2+ /Mg 2+ and incubated for 20 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed again with PBST and incubated with 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes, 1:2000 dilution) and phalloidin-tetramethyl rhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Systems Biology 2024Quote: ... The islets were then incubated for 1h at 4 ℃ with Goat anti-Guinea pig immunoglobulin G (IgG) (H+L) secondary antibody (DyLightTM 488) (1:1000, SA5-10094, Invitrogen), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were split (at 1:6) every 4 to 5 days by treatment with Accutase (Thermo/Life Technologies; A1110501). The minimize cell loss ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibody incubation was overnight at 4°C followed by incubation with secondary antibodies in blocking buffer overnight at RT (1:1000, ThermoFisher). Nuclei were stained with 4′,6-diamidino-2-phenylindole ...
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bioRxiv - Biophysics 2024Quote: ... then incubated for 30 min at 4°C with DPBS (+Ca2+/Mg2+) containing 0.5 mg mL-1 EZ-link Sulfo-NHS-SS-biotin (Thermo Scientific). Biotinylation was quenched by aspirating the biotinylation solution and incubating twice for 5 min with DPBS (+Ca2+/Mg2+ ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then blocked in 4% BSA in PBS followed by incubation in primary anti-V5 monoclonal antibody (mouse, 1:500, Invitrogen) in 4% BSA in PBS at room temperature for 45 min ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with PBS and incubated with Alexa-Fluor488-conjugated secondary anti-mouse antibody in 4% BSA in PBS at room temperature for 45 min (goat, 1:700, Invitrogen), followed by PBS washes ...
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bioRxiv - Biochemistry 2024Quote: ... were grown in DMEM high glucose (4 g/L) media supplemented with 10% FBS and 1% PSQ (Gibco, Waltham, MA). The day before transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary staining was performed overnight at 4°C using Alexa Fluor goat anti-mouse antibody (Thermo Fisher, A21235, 1:500). Alexa Fluor Plus 555 Phalloidin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1 or 3 mg of protein was incubated for 1 hour at 4°C with 10 μl pre-washed dynabeads (Invitrogen) to clear the lysate from unspecific interactions ...
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bioRxiv - Cell Biology 2024Quote: ... and loaded at the equivalent of 5×106 parasites/lane (unless otherwise indicated) on a 1-mm thick 4%-12% Bis/Tris NuPage polyacrylamide SDS gel (Invitrogen). Axenic strains of Dictyostelium discoideum (Table 1 ...
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bioRxiv - Genetics 2024Quote: ... The tissue sections were permeabilized with 1% Triton X-100 and blocked with 4% BSA in PBS for 20 minutes at RT and then incubated overnight at 4°C with the following primary antibodies: rabbit anti-MYO7A (1:400 dilution, PA1-936, Invitrogen), mouse anti-SOX2 (1:400 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were usually passaged every 3-4 days in a 1:10 ratio using 0.05% (v/v) trypsin (Gibco, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... Then day 4 aggregates containing hPGCLCs were transferred onto Nunc™ 4-Well Dishes (Thermo Fisher Scientific) containing 1× Protein Label solution ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed for 30 minutes at 4°C with 4% paraformaldehyde in PBS (Thermo Fisher Scientific), briefly washed three times ...
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bioRxiv - Genomics 2024Quote: ... the cells were loaded with the calcium indicator Fluo-4 (Fluo-4 NW Calcium Assay Kit, Invitrogen) and 500 nM SiR-tubulin Kit (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: 4×10^4 hESCs were seeded on 0.5 μg/cm2 Vitronectin-coated 12-well plate (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted separately from testes (n = 4) and ovary (n = 4) tissues using TRIzol (Invitrogen). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) or 12% Bis-Tris protein gel (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) for 1 h at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Stroma was thoroughly minced and incubated in 4 mg/ml collagenase type 4 (Thermo Fisher Scientific; 17104019) in DMEM with 1 U/ml RNAse inhibitor for 1 h at 37°C in a rotator ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted separately from testes (n = 4) and ovary (n = 4) tissues using TRIzol (Invitrogen). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... grown to confluence and incubated with the calcium indicator Fluo 4-AM (Fluo-4 Direct assay kit, Invitrogen) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... external cysteine residues were blocked with 107 μM 4-acetamino-4’-maleimidylstilbene-2,2’-disulfonic acid (AMS, Thermo Scientific) for 20 min on ice ...
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bioRxiv - Bioengineering 2022Quote: ... 4-Chlorobenzenesulfonate Salt (DID) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). Ammonium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Molecular Biology 2020Quote: ... or separating the pooled samples on a 4% agarose gel (E-Gel® EX Agarose Gels, 4%, Invitrogen), and gel purification of fragments larger than adapter dimers (>150bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Brain halves were either drop-fixed in phosphate buffered 4% paraformaldehyde 4°C (Thermo Fisher Scientific, Waltham, MA) for 24 hours at for immunohistochemical staining or micro-dissected (cortex ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of 100 μl of sample was loaded onto 4-12% Novex Tris-glycine gel (Invitrogen, XP04120BOX). Western blotting was done using an anti-FLAG antibody ...
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bioRxiv - Bioengineering 2023Quote: ... Fluo-4 reagent solution (prepared according to manufacturer’s instructions; Fluo-4 Direct Calcium-Assay-Kit, Thermo Fisher Scientific) was added to culture medium (50% of culture medium volume ...