Labshake search
Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... The cells were washed 4 times with PBST and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) at 1:1000 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... Fluo-4 reagent solution (prepared according to manufacturer’s instructions; Fluo-4 Direct Calcium-Assay-Kit, Thermo Fisher Scientific) was added to culture medium (50% of culture medium volume ...
-
bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
-
bioRxiv - Neuroscience 2023Quote: ... heated for 2min at 85C and loaded on a TrisGlycine 4-20% SDS-PAGE gel (4-20%, Invitrogen) along with a PrecisionPlus Kaleidoscope protein ladder (BioRad 1610375) ...
-
bioRxiv - Genetics 2024Quote: ... and BSA (3%) for 4 hours at 4°C after which streptavidin-agarose UltraLink Resin (Thermo Fisher Scientific) for 1 hour at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Gastruloids were subsequently fixed in 10 ml 4% paraformalde-hyde solution (PFA, Thermo Scientific Chemicals, 30525-89-4) for 2 h ...
-
bioRxiv - Biochemistry 2024Quote: ... with IonPac 4 x 250 mm IonPac AS4A and 4 x 50 mm AG4A guard columns (Thermo Scientific). Samples were eluted at 24 °C with 0.1 M NaOH (isocratic ...
-
bioRxiv - Plant Biology 2020Quote: ... two complementary oligonucleotides with 4-bp overhangs (Supplemental Table 1) were annealed and inserted by a Golden Gate reaction with BpiI (Thermo Scientific) and T4 DNA ligase (Thermo Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and incubated over night at 4°C with primary antibody mouse IgG2a anti-V5 tag monoclonal antibody (1/1000; Thermo Fisher Scientific Cat# R960-25 ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Immunology 2021Quote: ... NK cells were washed once in 1 x PBS and placed into Opti-MEM reduced serum media and into 4 mm electroporation cuvettes (Thermo Fisher) with 5ug mRNA/1 million NK cells ...
-
bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
-
bioRxiv - Developmental Biology 2020Quote: ... primary antibody staining was performed for overnight at 4°C on the shaker and secondary donkey anti-goat 568(1:400, Molecular Probes) was added following four washes of 0.3% PTX ...
-
bioRxiv - Neuroscience 2021Quote: ... the samples were incubated at 4°C overnight with goat anti-mouse AlexaFluor 488 nm (1:500) and anti-rabbit AlexaFluor 647 nm (Molecular Probes) in PBS-T ...
-
bioRxiv - Molecular Biology 2021Quote: ... was carried out overnight at 4° C followed by secondary antibodies (conjugated with Alexa Fluor 488 or Alexa Fluor 647 (1:250; Molecular Probes, Invitrogen) at RT for 4-5 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The diluted supernatants were pre-cleared for 1 hr at 4 °C on a rotator with 50 μL of Protein-A Dynabeads (Life Technologies) pre-blocked overnight in ChIP buffer + 0.01% SDS and 100 μg ultrapure BSA (Ambion) ...
-
bioRxiv - Molecular Biology 2021Quote: The cDNA reactions were diluted 1:50 and 4 µL was used per 10 µL qRT-PCR reaction with PowerUp SYBR Green Master Mix (ThermoFisher, A25742) and final primer concentrations of 500 nM in 384-well plates (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
-
bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
-
bioRxiv - Biophysics 2020Quote: ... by using a 15 ml douncer and cleared by centrifugation (186,200 × g, 1 hour, 4 °C, T865 rotor (#51411, Thermo Fisher Scientific)) ...
-
bioRxiv - Genomics 2020Quote: ... for 1 h at 4 °C and immunoprecipitations were performed with 30 μL of Pierce Anti-HA Agarose (Thermo Fisher Scientific) per sample rotating overnight at 4 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... Biotinylated mPA was then modified with 10 equivalents of sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC; Thermo Fisher Scientific) at r.t ...
-
Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... was measured by live ratio-fluoresce microscopy of ATT pre-treated cells after incubation in 80 µl/well of DPBS supplemented with 1 µM di-4-ANEPPDHQ (D36802, Thermo Fisher) for 30 min at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... actin cortices were staining by overnight incubation at 4°C in 1:100 rhodamine-phalloidin (stock in methanol; Fisher Scientific #10125092). For TUNEL staining using the “In Situ Cell Death Detection Kit TMR” (Roche #12156792910) ...
-
bioRxiv - Immunology 2021Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti–mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... and the expression plasmid encoding the GP gene of an EBOV Makona strain (GenBank accession no. KJ660346) or BDBV Uganda strain (GenBank accession no. KR063673) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher). After 48-72 h ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific) and mouse anti-SST antibody (1:250 ...
-
bioRxiv - Biochemistry 2021Quote: The cells were plated at 4 × 104 cells well-1 in a Nunclon Delta-Treated 96-well plate (Thermo Scientific 167008). After allowing an overnight growth for cells to adhere ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
-
bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
-
bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000; cat# 51-6900, Invitrogen/ThermoFisher, Waltham, MA) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in serum before incubation with mouse monoclonal β-endorphin primary antibody (Abeam ab54205,1:200) for 24 h at 4°C and Alexa-fluor 488 chicken anti-mouse secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were run on a 4%–12% polyacrylamide gel for 1 hour at 150 V and colored with PageBlue Protein staining solution (Thermo Scientific) overnight.
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... or 1 cm 4-12% Bis-Tris gel for ProQ diamond stain with 1x MOPS running buffer (Thermo Fisher Scientific, NP0001), transferred onto a PVDF membrane (EMD Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
-
bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
-
bioRxiv - Microbiology 2020Quote: ... the samples were purified to obtain only the bacterial cells by first diluting them 1/4 in UltraPure™ DNase/RNase-Free distilled water (Invitrogen), then centrifuging twice at 4 226 g for 10 min ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The lysate was clarified by centrifugation (18 000g, 30 min, 4°C) and applied to a 1-ml Ni-NTA column (HisPur, ThermoFisher Scientific). After incubation at 4°C for 1 h the resin was washed with 50 ml of lysis buffer and eluted with 6 ml lysis buffer plus 250 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
-
bioRxiv - Microbiology 2020Quote: GTPases at a final concentration of 10 μM were loaded with sub-stoichiometric (1/4 [GTPase]) concentrations of GDP-BODIPY-FL (Thermo Scientific) in the presence of 50 mM Tris pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were fixed in ice-cold 100% ethanol for 1 minute followed by 4 minutes of staining with 0.0001% Cresyl Violet (ACROS Organics, AC229630050). Slides were dehydrated using 95% and 100% ethanol followed by xylene for 1 minute each ...
-
bioRxiv - Microbiology 2021Quote: ... The sonicated cells were centrifuged at 14,000 rpm for 1 h at 4°C to remove the cell debris and the supernatant was incubated to Ni2+-NTA resin (Thermo Fisher) pre equilibrated with lysis buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...