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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Blots were incubated with primary antibody for either 1 hour at room temperature or at 4°C overnight using the following dilutions: 1:1,000 mouse-α-HA 2.2.14 (Thermo Fisher 26183), 1:4,000 rabbit-α- PfClpP and 1:4,000 rabbit-α-PfClpR47 (kind gifts from Walid Houry) ...
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bioRxiv - Cell Biology 2020Quote: ... before incubating with 4 μg/ml of secondary antibodies for 1 h at room temperature (Alexa fluro 488, 594, 647, Invitrogen). Samples were imaged on an Olympus FV3000 confocal laser scanning microscope.
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4°C with an antibody against either Perlecan (1:50, Life Technologies, MA1-06821, Carlsbad, CA), Collagen2a1 (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... and further cultured in the DMEM/F12-based medium (D-MEM/F12 supplemented with 4% KSR additive, 1% ITS-X (Gibco), 0.3 % BSA (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... double-stranded RNA was adjusted to 4 μg/μL and mixed with Rhodamine Dextran B dye (20:1) (Thermo Fisher) for visualization of injected volume ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with goat anti-rabbit Alexa Fluor 488 secondary antibody (used 1:800 dilution, A27034 Invitrogen). Finally ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Biophysics 2022Quote: ... was dialyzed overnight in 1 L of 1 × TB at 4 °C in a Slida-A-lyzer mini dialysis unit with 3,000 MWCO (Thermo Fisher) to reduce the amount of glycerol in the sample ...
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bioRxiv - Cell Biology 2022Quote: ... cells were kept overnight at 4°C in IMDM medium supplemented with 10% FBS (HyClone) and 1% penicillin-streptomycin (Thermofisher). Staining was performed for 20min at room temperature (RT ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 μL of boiled beads (representing 4 %) in 1 x NuPAGE loading dye were loaded onto NuPAGE Bis-Tris gels (MW<100 kDa) (Invitrogen) or Tris-Glycine gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde-PBS for 20 minutes at 37°C then stained in 1:2000 diluted Hoechst 33232 (ThermoFisher) for 10 mins.
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO] ...
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bioRxiv - Cell Biology 2022Quote: ... BJ-5ta cells (CRL-400, ATCC) were cultured in a 4:1 mixture of DMEM and Medium 199 (Thermo Fisher) containing 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C and further incubated with secondary antibodies (goat anti-rabbit Alexa fluoro 488, Invitrogen, 1:200 dilution). The nuclei were stained with DAPI (1:1000) ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... were filleted using standard protocols and fixed in 4%PFA for 15min and stained with a rabbit anti-GFP antibody (1:500, Invitrogen). Secondary antibodies conjugates with Alexa488 ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated with primary antibody overnight at 4°C followed by a 10min DAPI stain (1:30,000 at room temperature, Invitrogen, D1306) and 1h incubation with secondary antibodies including ...
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bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
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bioRxiv - Cell Biology 2021Quote: ... The obtained protein samples were separated on 4-12% Bis-TRIS NuPAGE gel in 1 × MES buffer (Thermo Fisher Scientific) and transfer to nitrocellulose membrane before blocking with 5% non-fat milk dissolved in 1 × TRIS-buffered saline (TBS ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... at 4°C for overnight and Alexa conjugated secondary antibodies (1:1000, Goat anti-rabbit IgG Alexa fluor 647, Invitrogen, A21245 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: A stable dispersion of SWCNTs was achieved based on previously published protocols28,43 through tip-sonication for 1 h at 30 % power at 4 °C (Fisher Scientific Model 120 Sonic Dismembrator ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Cancer Biology 2021Quote: 20 µg of proteins extracted in SDS buffer (1% SDS supplemented with protease and phosphatase inhibitors) were loaded on to a 4-12% gradient SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... PBMC1-2 and MS-5 cells were cultured alone and in co-culture in a 4:1 AML/PBMC-stromal ratio and 500,000 cells/mL density in α-MEM (Gibco) with 12.5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C and then with 1:250 goat anti-mouse Alexa Fluor 488 (A11008; Life Technologies, Waltham, MA) and 1:250 goat anti-rabbit Alexa Fluor 647 (A21244 ...
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bioRxiv - Plant Biology 2021Quote: ... a mouse anti-FLAG primary monoclonal antibody was used (anti-DYKDDDDK FG4R, 1 μg/mL overnight at 4 °C, Invitrogen), and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C ...
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bioRxiv - Microbiology 2020Quote: Purified cysts were stained following brain tissue homogenization using a 1:4 dilution of 12.3mg/ml of Hoechst stain (Thermo Scientific) and incubated for 20-30 minutes prior to being viewed on a Zeiss Axiovert microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Wells were washed with PBS and stored overnight at 4°C in EL08.1D2 cell culture media supplemented with 1% P/S (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... bacteria from 1 ml samples were harvested by centrifugation and resuspended in 500 μL of FM™ 4-64FX (Invitrogen) at 5 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: Culture medium was replaced with Fluo-4 AM (5 μM) or X-Rhod-1 AM (10 μM) (both from Invitrogen) working solution in Ca2+ solution (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: 8 x 103 HeLa-ACE2 cells and 3.2 x 104 HeLa or HeLa-sgLRRC15 cells (1:4 ratio) were co-plated per well in 8-well chamber slides (Nunc). The following day ...
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bioRxiv - Immunology 2020Quote: ... Twenty-four h post transfection MEFs and HEK293Ts were treated with 4 μg/ml and 1 μg/ml puromycin (Invitrogen) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... 20-25 µg of RIPA or SDS lysate or 1 µg of S4 lysate were separated in a 4-20% Tris-glycine gel (Novex, Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Biophysics 2020Quote: ... Samples were heated to 90°C over 1 hr and then slowly cooled overnight to 4 °C (−1°C per 20 minutes) using a Thermocycler (ProFlex PCR System, ThermoFisher). Refolded samples were resuspended through pipetting and combined into a fresh 1.5 mL microfuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating brain sections were incubated overnight at 4°C with primary anti-FSHR (1:200; catalog # PA5-50963, ThermoFisher), anti-NeuN (1:300 ...
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bioRxiv - Genomics 2022Quote: ... 80% confluent flasks of HEK293FT cells were split 1:4 into DMEM culture medium [10% Fetal Bovine Serum (cat. #10437-028, Gibco), 1% PenStrep (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with either mouse anti-mitochondria antibody (Thermo Fisher Scientific, 1:200, # MA5-12017); mouse anti-nuclei antibody (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA prepared thus was diluted 1:60 and 4 µL of the diluted cDNA was used for each qPCR reaction with SYBR green (Invitrogen). β-actin was used as the reference endogenous gene for normalization ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS, and stained using anti-IgG AF647 (1:600 dilution in MACS, 30 min at 4°C) (ThermoFisher). Cells were then fixed using PFA 4% ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_10541045) for 3 days at 4°C followed by 2h in 1:800 anti-mouse FluoroNanogold (Life Technologies, A24920) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... then washed three times with PBS and incubated with secondary antibodies (Table 4) and phalloidin (F-actin detection) (1:100) (Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated in the following secondary antibodies diluted to 1:1000 in ovary block overnight at 4°C: goat anti-rabbit IgG Alexa Fluor 633 (Invitrogen), goat anti-mouse IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were probed overnight at 4°C using the following primary antibody dilutions: rabbit anti-GFP (1:1000, Invitrogen #A11122), and mouse anti-brp (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were labeled overnight at 4 °C with Alexa Fluor 488-labeled wheatgerm agglutinin (1:200; W11261, Thermo Fisher) in combination with a rabbit polyclonal primary antiserum ...