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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792, 1:500; Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Plant Biology 2023Quote: ... 4 μl of the DNAseI reaction was mixed with 1 μl of oligo(dT)18 0.5 μg/μl (Thermo Scientific), and incubated at 65°C for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4°C followed by incubation with donkey anti-goat Alexa Fluor 594 secondary antibodies (1:200, A-11058; Invitrogen) and rabbit anti GFP (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 24 hours at 4 °C with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:300, A-11058, Invitrogen), Cy3-conjugated goat anti-rabbit IgG (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the tissues were washed five times with PBS and then permeabilized for 1 hour with 0.2% TritonX-100 supplemented with 4% goat serum (Thermo Fisher). The permeabilization solution was also used to generate a primary antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... before being incubated 72hours at 4°C with the following primary antibodies: chicken anti-GFP (1:1000, Life Technologies, #A10262) and rabbit anti-FMRP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... WAKO) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rabbit secondary antibody (1:1000, Life Technologies), and Alexa 594 conjugated anti-rat secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041 ...
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred either to nitrocellulose or PVDF membranes (Invitrogen) ...
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bioRxiv - Pathology 2024Quote: ... at 4 °C overnight followed by an Alexa Fluor 594-conjugated secondary antibody (1:500) (A-11012, Thermo Fisher Scientific). Immunofluorescence staining images were acquired using the Pannoramic Scanner software (v3.0.3.139795 RTM) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated overnight at 4°C in a humidified chamber with anti-γ-H2AX (1:500, Invitrogen MA5-38225), anti-perilipin-2 (1:200 ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were drawn after 0.5, 1, 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was dialysed in 1 L of purification buffer at 4°C using Snakeskin Dialysis Tubing (3.5 k MWCO, Thermo Fisher), followed by an overnight dialysis under the same conditions.
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified at 4°C by the batch method over 1 mL Ni-NTA resin according to the manufacturer’s instructions (HisPur, Thermo Scientific). All buffers were 50 mM NaPO4 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an overnight incubation at 4 °C with 20 μg ml-1 laminin (Thermo Fisher Scientific, 23-017-015). After being thoroughly washed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000; overnight at 4 °C; # A-11035, Invitrogen). Sections were mounted in a medium containing 50 % glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C in a humified chamber with anti-AKAP12 antibody (Thermo Fisher #PA5-21759, 1:200) or anti-Ki67 (CST #9449 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated rotating for 1 h at 4 ºC on 1.25 mL bed of HisPur Ni-NTA agarose beads (ThermoFisher Scientific), pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Myoblasts were cultured in growth medium consisting of 1 vol 199 Medium/4 vol DMEM (Life Technologies, Carlsbad, CA, USA) supplemented with 20% fetal calf serum (Life technologies ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 4 hours with Streptavidin AlexaFluor-488 (S32354) and anti-mouse CY5 (A10524) (ThermoFisher Scientific; 1:1,000) where appropriate in a 5% NGS and 0.25% Triton-X in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Developmental Biology 2023Quote: ... A total of 10 washes with PBSFT were performed before secondary antibodies (diluted 1 in 500) (see Table 4) and Hoechst (Hoechst 33342 trihydrochloride trihydrate, Invitrogen Mol Probes H3570 ...
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bioRxiv - Biophysics 2023Quote: Cryo-EM data collection for the hCLDN-4/cCpE/COP-1 complex was performed on a Titan Krios G3i (ThermoFisher) equipped with a Gatan K3 direct electron detector and BioContinuum GIF at PNCC ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in primary antibody in 5% milk and TBS-T overnight at 4°C at the following dilutions: 1:1000 rabbit αFLAG polyclonal (Invitrogen) and 1:1000 rabbit αHsp70 polyclonal (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellets were then concentrated at 70,000 x g for 1 hour at 4°C and gently resuspended in 140 ul of DPBS (Gibco; 14190144) and allowed to resuspend overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed again in 0.5% Triton PBS and incubated for 2 h at room temperature or overnight at 4 °C in secondary antibody (donkey anti-goat 647, 1:1000, A-21447, ThermoFisher) in carrier solution ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: ... Blotted membranes were blocked with PBS containing 4% w/v skimmed milk powder followed by incubation with 1:1000 dilution of mouse anti-GFP antibody (GF28R, Invitrogen) in blocking buffer followed by incubation with 1:5000 dilution of goat anti-mouse IgG secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Biochemistry 2022Quote: ... Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher) at room temperature prior to use.
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...