Labshake search
Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... To 32 μl of the cleared cell extract 4 μl of FastAP phosphatase (1 U/μl, ThermoFisher Scientific, Dreieich, Germany) and 4 μl of 10-times FastAP buffer were added ...
-
bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
-
bioRxiv - Cell Biology 2023Quote: Plasmids were transiently transfected to HEK293T cells for transgene expression using FuGENE (4 μL FuGENE per 1 μg of DNA) in 100 μL of Opti-MEM (Gibco) per well of a six-well plate (2 μg DNA total per well) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then incubated overnight at 4°C in a humidified chamber with anti-γ-H2AX (1:500, Invitrogen MA5-38225), anti-perilipin-2 (1:200 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
-
bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
-
bioRxiv - Biochemistry 2022Quote: ... C6 cells were grown and maintained in High Glucose DMEM (25 mM glucose, 1 mM pyruvate, 4 mM glutamine; #12800017, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
-
The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
-
bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated with anti-IgM antibody (1/100) (Jackson Immuno Research) for 30 min at 4°C and mounted in Fluoromount-G with DAPI (Invitrogen). Fluorescence was detected using an inverted confocal microscope ...
-
bioRxiv - Microbiology 2022Quote: ... Total lipids were extracted by resuspending washed cell pellets in 1 mL HPLC grade methanol (A454-4, Thermo Fisher Scientific), transferring to a glass vial with PFTE cap ...
-
bioRxiv - Neuroscience 2022Quote: ... and spinal cord medium (MEM with Glutamax [Gibco] supplemented with 4 mg/ml Albumax [Gibco], 1 mM pyruvate [Sigma], 100 units/ml Penicillin and 100 μg/ml Streptomycin [Gibco]), as previously described (Dumoulin et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... The plasmids were mixed with 100 μl of PEI (4:1 v/w PEI:DNA ratio) in 500 μl of pre-warmed OptiMEM II reduced serum medium (Gibco, 1985070), after 10-15 min incubation at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... the culture was fixated by removing the remaining medium and adding 1 mL of a 4% paraformaldehyde (PFA, Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792, 1:500; Thermo Fisher Scientific Cat# A-21203 ...
-
bioRxiv - Plant Biology 2023Quote: ... 4 μl of the DNAseI reaction was mixed with 1 μl of oligo(dT)18 0.5 μg/μl (Thermo Scientific), and incubated at 65°C for 5 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher) at room temperature prior to use.
-
bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
-
bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
-
bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting BHK cells (ATCC) or Phoenix cells (laboratory stocks) with 1-4 μg plasmid using the Lipofectamine 2000 Transfection Reagent (Invitrogen). Supernatants were collected at 24 and 48 hours post-transfection and added to MEFs pre-cultured in six-well plates ...
-
bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
-
bioRxiv - Neuroscience 2023Quote: ... rinsed 3X in 0.1% PBST and incubated in goat anti-mouse Alexa Flour 647 antibody overnight at 4°C (1:300, ThermoFisher A32728). Images were acquired using a Zeiss 880 Laser Scanning Confocal inverted microscope with a Plan-Apochromat 63x/1.4 oil DIC M27 lens ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved by SDS-PAGE (approximately 1 × 107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
-
bioRxiv - Biochemistry 2023Quote: ... 200 mM Tris-HCl, pH 8.0, 8 M Urea, 4% CHAPS, 1 M NaCl, supplemented with protease and phosphatase inhibitors, Thermo Scientific) was added to the pellets and the extracts were sonicated 10 x 3s with a probe sonicator ...
-
bioRxiv - Biochemistry 2022Quote: ... diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054, Invitrogen) diluted in PBS-T during 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies: TMEM163 (Thermofisher, cat # PA5-114329; 1:100), CD83 (Biolegend ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the membrane was incubated overnight at 4°C with primary antibody solution (1:1000 dilution for anti-TSG101 [Abcam, ab30871], anti-Calnexin [ThermoFisher, PA5-19169] and anti-Syntenin-1 [Origene ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were spun at 100,000xg for 1 hour at 4°C using Sorvall Discovery MX120SE Ultracentrifuge (Thermo Scientific; Waltham; MA) to isolate the cytosolic fraction ...
-
RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the cells were counted and incubated for 45 min at 4°C with EMCN antibody (#2114518, Invitrogen, USA, 1:100) and CD31 antibody (#FAB6874G ...
-
bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
-
bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
-
bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
-
bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by corresponding Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (1:500; Invitrogen) or anti-αSMA-Cy3 antibody (Sigma Aldrich ...
-
bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated at 95 °C for 10 min then centrifuged at 14,000 xg for 1 min before loading on Novex 4-20% Tris-Glycine gel (Invitrogen, XP04205). After transfer to PVDF-FL (Merck Life Sciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
-
bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
-
bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and a maximum amount of proteins (2.3-4 mg) was loaded on 1 mg of Dynabeads M-280 Streptavidin (ThermoFisher Scientific), previously equilibrated with RIPA buffer ...
-
bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... and then were incubated overnight at 4 °C with Alexa Fluor 488-labeled secondary antibody (Fisher Scientific, A32723, 1:200), and mounted with DAPI (Vector Laboratories) ...
-
bioRxiv - Neuroscience 2024Quote: ... retinas were incubated with secondary antibodies at 4°C for another 2-3 days: goat anti-chicken antibody conjugated to Alexa Fluor 488 (1:1000; A11039, Invitrogen), goat anti-rabbit 555 (1:1000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... were immediately mixed with 0.5 µl fMet-[Cy5]tRNAfMet (4 µM) and electroporated in a 1-mm electroporation cuvette (Thermo Scientific) with a MicroPulser Electroporator (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4 °C in the dark with Hoechst 33342 (1:10,000; Thermo Fisher Scientific, Cat # H3570) for nuclear staining ...