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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Midguts were incubated with primary antibody anti-flavivirus envelope 4G2 antibody produced in-house in 1X PBS at 4 °C overnight followed by 1:500 secondary antibody anti-mouse IgG Alexa 488 (Invitrogen, A28175) in 1X PBS and Hoechst33342 (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and 4 µL (<1 µg) of DNase treated total RNA was then reverse transcribed into cDNA (Superscript III Supermix, Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody solution for 4 hours at RT (5% normal goat serum in PBST, Alexa Fluor 633 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated for 30 min at 4 °C with AF647-conjugated goat anti-mouse IgG (1:500, Thermo Fisher). Cholesterol levels were determined by incubation for 30 min at 4 °C with filipin (50 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Part of the supernatant was conserved as total lysate and the remainder was subjected first to a preclearing step with agarose for 1 h at 4°C and then to pull-down assay with NeutrAvidin agarose (Thermo Scientific) for 2 h at 4°C to isolate biotinylated proteins.
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 30 minutes in a live/dead Viability/Cytotoxicity solution containing 4 mM Calcein and 8 mM Ethidium homodimer-1 (L3224, ThermoFisher Scientific) at 37 °C ...
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bioRxiv - Pathology 2024Quote: ... pH 7.4 and 500 mM NaCl, 20 mM imidazole, 1 mM PMSF, protease inhibitor cocktail Set III, EDTA free from Thermo Fisher) and lysed by an LM20 microfluidizer (15000 psi) ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti-mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed with 5 mL PBS (4°C) and trypsinized with 1 mL 0.25% Trypsin-EDTA per dish (Gibco, 25200-056) for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... A172 cell line was cultured in high glucose DMEM media plus 4 mM L-glutamate and 1 mM sodium pyruvate (Thermo Fisher) supplemented with 10% FBS and 1% penicillin and streptomycin mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4°C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496, ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... lysed and sonicated using Diagenode Bioruptor® Pico sonicator for 10 cycles with alternating 30 secs on/ off at low intensity and 1 mg of protein was digested with 4 U of Turbo DNase (Ambion, AM2238) and 0.28 U/ml (low ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,812 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,650 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Physiology 2024Quote: Intracellular Ca2+ [Ca2+]i measurements were performed in cardiomyocytes loaded with Fluo-4 AM dye (5 μmol.L-1, ThermoFisher, Cat #F14201). After a 30-minute loading period ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with an anti-HIV-1 p24 monoclonal (AG3.0) primary antibody (4 µl/mL) (NIH HIV Reagent Program catalog# 4121) or P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... further supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) and 25 mM 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPEs) buffer (Gibco, US), 2.5g/l Glucose (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... The fixed cells were spun down at 900 × g for 5 min at 4°C and then washed twice with 1 ml of ice-cold PBS (Gibco 70011044). Cells were lysed twice with 1 ml of ice-cold 4C permeabilization buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... the media was removed and cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) at a concentration of 1 µg/mL (ThermoFisher Scientific) in Phosphate-Buffered Saline (PBS ...
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bioRxiv - Genomics 2024Quote: ... was denatured with LDS Sample Buffer and Sample Reducing Agent and separated on 4-12% Bis-Tris 1 mm mini protein gels using MES SDS Running Buffer with 0.1% NuPAGE Antioxidant (Invitrogen Cat#NP0005). Molecular weight was assessed using the eBlue Plus2 Prestained Protein Standard (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... followed by overnight incubation at 4°C with rabbit-primary antibodies anti-mouse Perforin (1:50) (PA5-17431, Thermo Scientific. USA) or anti-mouse α-actin (1:100 ...
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bioRxiv - Biophysics 2024Quote: Aliquots of 3 μL of complex sample was applied on UltrAuFoil r1.2/1.3 Grids covered with Graphene Oxide as described before76 and the grids were blotted for 3.5 s at force -1 at 4°C and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 26,386 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... and then treated or not with RNAse I (0.3 – 1 U) in combination with TURBO™ DNase I (4 U; Invitrogen, #AM2238) and incubated at 37°C for 3 minutes at 800 rpm ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... sections were incubated with diluted primary antibodies (as per manufactures recommendation) at 4°C overnight (IBA1 antibody; Cat #NC9288364; 1:1000; Fisher Scientific, GFAP Polyclonal Antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4°C: AT8 (Mouse, 1:100, Thermo Scientific), and Iba1 (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Adult flies were fixed with 4% paraformaldehyde in 1X PBS for 1 hour after a quick dissection,then immersed in OCT compound (Fisher Scientific) and flash frozen in liquid nitrogen or dry ice ...
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bioRxiv - Immunology 2024Quote: ... the slices were incubated for 24 hours at 4°C with Alexa Fluor 488 secondary antibody (1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Biochemistry 2024Quote: A total of 1 μg of plasmid DNA (TLR3:minibinder-protein A ratio of 1:4) was transfected into 2.5×106 Expi 293F cells (#A14527, Thermo Fisher Scientific) using Expifectamine (#A14524 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 9,018 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 8,294 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Developmental Biology 2024Quote: ... The fixed ovaries were washed in washing buffer (WB, 1% BSA in 1X PBS) thrice before being embedded into 4% low-melting point agarose (Thermo Fisher). The embedded tissue was sliced into 100 µm thick tissue sections using a vibratome (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed and incubated overnight at 4°C with secondary antibodies Goat-anti-Chicken 488 (1:400, Thermo Fisher Scientific Cat# A-11039 ...
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bioRxiv - Developmental Biology 2024Quote: ... for one hour at RT and stained overnight at 4 °C with primary antibody Rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Neuroscience 2024Quote: ... Neurobiotin-injected specimens for the tracer-coupling analysis of HCs were first incubated overnight at 4°C with Alexa568-conjugated streptavidin (1:250, Invitrogen, S11226), diluted in incubation buffer containing 10% NDS ...
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bioRxiv - Microbiology 2024Quote: ... Beads were washed three times with 500 µl of IP buffer (500 x g, 1 min, 4°C) and resuspended in 65 µl of NuPAGE LDS-Sample buffer (Thermo Fisher) supplemented with 100 mM DTT ...
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bioRxiv - Immunology 2024Quote: ... CAL-1 cells were infected with Salmonella for 4 h and then incubated for 30 min at 37 °C with CellROX Deep Red Reagent (1 µM, #C10422 Invitrogen Thermofisher) for total (cytoplasmic and nuclear ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions 1-4 were pooled and concentrated using centrifugal filters units (30 kDa MWCO, MilliporeSigma Amicon Ultra Centrifugal Filter Unit, Fisher Scientific) at 4000 × g for 30 min to a final volume of 100uL.
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bioRxiv - Plant Biology 2024Quote: ... the middle region of fully expanded leaf 4 and 8 was fixed with 1% (w/v) glutaraldehyde (Thermo Fisher Scientific Inc.) in 1X PBS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were stained overnight at 4⁰C with 1:100 dilutions of the following antibodies: p-tau antibody (Thermofisher, AT8) recognizing p-tau Ser202/Thr205 ...
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bioRxiv - Immunology 2024Quote: Two million splenocytes in 200 μl PBS from WT-Foxp3YFPCre/YFPCre and Dgka-/-zf/f-Foxp3YFPCre/YFPCre mice were seeded into a U-bottom 96-well plate in the presence or absence of 100 μM 2-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; Life Technologies). After incubation at 37°C with 5% CO2 for 30min ...
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bioRxiv - Microbiology 2024Quote: ... with 5% dry milk before incubating on a rocker overnight at 4°C with either mouse anti-FLAG (1:5,000 dilutiion; Thermo Scientific, MA191878), rat anti-BB0346-HIS (1:5,000 dilution) ...
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bioRxiv - Bioengineering 2024Quote: ... Nuclei and F-actin were stained by incubation with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific) and phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were incubated in primary antibodies overnight at 4 °C and in secondary antibodies (Invitrogen #A11036 and #A32728, diluted 1:1000) for 2 hours at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Organoids were rinsed 3 x 2 hours in OWB on a rocking platform before incubating overnight at 4°C in 1:250 donkey anti-rabbit Alexa Fluor 555 (ThermoFisher; A31572). Organoids were rinsed for 1 hour in OWB before incubating at room temperature for 20 minutes in 1:2000 DAPI (Millipore Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... or cyt-mCh-GFP were generated by lentiviral transduction followed by selection using blasticidin (4 µg mL-1; Gibco, Themofisher Scientific) for 3 passages ...
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bioRxiv - Bioengineering 2024Quote: Coronal sections of healthy murine brain were prepared and stained as previously described101 either using 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng mL−1; Molecular Probes) to stain cell nuclei or primary antibodies for rabbit anti-NeuN (1:1000) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated for 1 additional hour at 4°C and then washed twice with FACS buffer (a 1x DPBS (Thermo Fisher) solution with 2.5% FBS and 0.02% sodium azide) ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was then incubated overnight at 4°C in an AFB solution with 1:200 Phalloidin (A12379, Thermo Fisher Scientific) and 1:1000 Hoechst 33342 (A3472 ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for at least 10 minutes at 72°C and loaded onto a 1 mm thick NuPAGE 4–12% Bis-Tris Mini Protein Gels (Thermo Fisher). Electrophoresis was performed for 2 hours at 100V in Thermo Fisher Mini Gel Tank or until the dye front migrated to the bottom of the gel ...