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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in 1 ml of staining buffer and 4 drops (164 μl) of Propidium Iodide Ready Flow (Invitrogen, R37169) was added to the cells and incubated at 25 °C for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Primary antibodies were incubated overnight at 4 °C before washing and staining with goat anti-rabbit AlexaFluor647 (1:500, Invitrogen A21235) and goat anti-human AlexaFluor488 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then washed with 0.1 M PBS (6×20 minutes at 4°C) and incubated with secondary antibodies conjugated to Cy3 and Alexa Fluor 488 (1:500, ThermoFisher, Germany) in 0.1 M PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split when they reached 80% confluency (every 2-4 days) using 0.5 mM EDTA in 1× DPBS (Thermo Fisher Scientific).
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... washed three times with TBS-T and probed at 4°C overnight with the following primary antibodies: MIC26 (Invitrogen, 1:1000), MIC27 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 4 and 5 we confined cells by using a soft acrylamide gel and either 1% Low Melting Agarose (Invitrogen, 16520-100) or a second soft acrylamide gel ...
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bioRxiv - Genetics 2023Quote: ... Cells were incubated with primary antibodies overnight at 4 °C and probed with the appropriate secondary antibodies for 1 hour at room temperature (ThermoFisher Scientific). Primary and secondary antibodies were resuspended in 0.2% Gelatin in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with the appropriate secondary antibody (Alexa Fluor 488 or 555 at 1/1000, Molecular Probes).
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min with PBS and incubated over-night at +4°C with goat anti-rabbit Alexa Fluor 564 (1:1000, Invitrogen, ThermoFisher Scientific) and goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 3x 10 min with PBS and incubated either for 2 hrs at RT or 24-72 hrs at 4° C in goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, ThermoFisher Scientific) secondary antibody in carrier solution containing 1% NGS ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (4 x 300 µL PBST) and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat ...
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bioRxiv - Neuroscience 2023Quote: ... 30 µg of TDP-43 protein extract or 50µg of RGNEF extract was then resuspended in 1 X NuPAGE LDS Sample buffer (4 X) (Thermo Fisher Scientific) and denaturated at 85°C for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... The samples were then stained with the blood lineage antibody panel (Table 1) for 30 minutes at 4°C and then washed with PBS (ThermoFisher Scientific) and 2% (v/v ...
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bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS 15 mins × 4 in a slide mailer and incubated in secondary antibody at 1:1000 dilution (goat anti-chicken 647, Themo Fisher Scientific Cat# A-21449 and goat anti-rabbit 546 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175 ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed three times for 10 min in PBS and incubated for 24 h at 4°C in secondary antibodies (donkey anti-rabbit Alexa Fluor 488, 1:250, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in the following primary antibodies overnight at 4°C: rabbit-anti-ZO1 (40-2200, Thermo Fisher Scientific, 1:200) and mouse-anti-RPE65 (MAB5428 ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Cell suspensions were concentrated to 1 × 106 cells/mL in PBS and centrifuged onto glass slides using a Cytospin 4 cytocentrifuge (Thermo Scientific). Fixed cells were permeabilized with 0.3% Triton X-100 in PBS for 5 min and incubated with Triton Block (0.2 M glycine ...
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bioRxiv - Microbiology 2022Quote: ... Lungs were inflated with 1 ml of a solution containing 1 part 4% PFA/30% sucrose and 2 parts of optimal cutting temperature compound (OCT) media (Fisher Scientific). Inflated lungs were tied off and stored in 4% PFA/30% sucrose at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were washed 4×20 minutes with PBST and then incubated for 30 minutes in Hoechst (1:2000; Thermo Fisher H3570). Samples were washed a final time in PBST and were stored in PBST until imaged.
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Genomics 2022Quote: ... Sorted cells were centrifuged at 300g for 10 minutes at 4°C and resuspended in 1 ml of TRIzol (Invitrogen, 15596018). Spermatogonial RNA was purified according to the standard TRIzol protocol and contaminant genomic DNA was digested using the DNA-free kit (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000; MA1-16616, AB_568420, clone P3UI, Thermo Fisher Scientific) and mouse anti-β-Tubulin E7 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were isolated by Folch extraction116 by adding 24 mL 2:1:0.6 HPLC chloroform (C297-4, Thermo Fisher Scientific): HPLC methanol (A454-4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
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bioRxiv - Genomics 2022Quote: ... and the resulting pellet was suspended in 0.85% NaCl (1.5 mL) and centrifuged (10,000 g, 1 min, 4°C) using Heraeus Primo R Centrifuge (Thermo Scientific, Schwerte, Germany). Pellets from the two tubes were dissolved in high salt buffer (1 mL ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated overnight at 4°C with a primary antibody directed against the V5-tag (ThermoFisher, 46-0705, 1:200) or against small double-stranded RNAs (J2 antibody ...
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bioRxiv - Biophysics 2023Quote: Fluorophores were prepared for linking into the gel by incubating them at 4°C overnight in 250 μl of PBS and 1% Acryloyl-X (w/v; Catalogue number A20770, Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed and stained for 48 h at 4°C with a 1:100 dilution of goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (A28175, Life Technologies), goat anti-rabbit secondary antibody conjugated to Alexa-Fluor 555 (A21428 ...
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bioRxiv - Cell Biology 2023Quote: Human nasal brushings were collected fresh for this study and immediately placed in a 15-ml sterile Falcon tube containing 4 ml of transport medium (αMEM supplemented with 1× penicillin–streptomycin (Gibco, 15070), 10 ng/ml gentamicin (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
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bioRxiv - Molecular Biology 2023Quote: ... was equilibrated with lysis buffer and then incubated with the cell lysate at 4 °C for 1 h on an end-to-end rotator (Cat# 88881001, ThermoFisher scientific). The slurry was transferred to a 15 ml falcon tube ...
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bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...