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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-EGFP (1:1000, Thermo Fisher# A-6455) and rabbit anti-CSPG (1:1000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... eGFP Monoclonal Antibody (MA1-952, Thermo Fisher Scientific, USA) was used as the primary anti-GFP Antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP and Cas9 using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... for eGFP or 100 µg/mL Hygromycin B (ThermoFisher) for NLuc ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Genomics 2020Quote: ... and fragmented by heating at 94 °C for 3 min in 5× First Strand Buffer (Invitrogen). The fragmented mRNAs were reversely transcribed into cDNA by SMARTScribe reverse transcriptase (TaKaRa) ...
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bioRxiv - Cell Biology 2022Quote: ... passage 3 fibroblasts were trypsinized 5 min at 37°C using 0.05% trypsin with EDTA (Gibco), centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.5°C increments) for 3 minutes in a gradient thermal cycler ProFlex PCR System (ThermoFisher Scientific) and immediately cooled down at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were heated at 54°C for 3 min in a thermal cycler (VeritiPro, Applied Biosystems). This temperature was previously selected as the temperature corresponding to the maximal thermal shift of UBA122 ...
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bioRxiv - Genomics 2023Quote: ... at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen) for 20 min to capture the antibody-protein complexes ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Systems Biology 2020Quote: ... and then 40 cycles of 95°C for 1 s and 60°C for 30 s in QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). PCR primers were designed using the Primer-BLAST tool available from the NCBI web site (57) ...
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bioRxiv - Microbiology 2021Quote: ... then 40 cycles of 94°C for 1 minute and 60°C for 1 minute on a QuantStudio 3 real-time thermocycler (Thermo Fisher Scientific, Waltham, MA, USA). KPPR1 genomic DNA was used as positive control and 100% reference for calculating Klebsiella relative abundances ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid harbouring eGFP-Rab11a-WT was obtained by mutagenesis of eGFP-Rab11a-DN construct with Phusion® High-Fidelity DNA Polymerase (Thermo Scientific, Cat # F530S) using primers as follows ...
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bioRxiv - Developmental Biology 2024Quote: Full length Trus protein N-terminally tagged with 6xHis-EGFP (His-EGFP-Trus) was expressed in Sf9 cultured insect cells using the Bac-to-Bac Baculovirus Expression System (Thermo Fisher Scientific, Waltham MA). The trus coding sequence ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were Anti-eGFP Rabbit (Invitrogen, 1:500) and anti-tRFP Rabbit (Evrogen ...
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bioRxiv - Bioengineering 2020Quote: ... CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher) with 10% HI-FBS ...
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bioRxiv - Genetics 2021Quote: ... Parallel transfection with an eGFP expression plasmid (240 ng; Invitrogen) was used to determine nucleofection frequencies (>90%) ...
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bioRxiv - Cell Biology 2024Quote: ... EGFP constructs transfection were performed using Lipofectamine® 2000 (Invitrogen). For SMARCA2 or SMARCA4 knockout ...
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bioRxiv - Synthetic Biology 2023Quote: ... a monoclonal Anti-eGFP antibody raised in mouse (MA1.952, Invitrogen) was used.
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bioRxiv - Neuroscience 2023Quote: ... after the anti-eGFP primary antibody (1:15000, ThermoFisher, #A10262) we used ABC (avidin biotinylated horseradish peroxidase complex ...
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bioRxiv - Biophysics 2022Quote: ... the EGFP HEK293A report cells were cultured in DMEM (Gibco) medium supplemented with 10 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... denaturation for 2 min at 95 °C followed by 45 cycles of 3 s at 95 °C and 30 s 60 °C on the StepOnePlus™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). All samples were run in triplicate ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
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bioRxiv - Neuroscience 2022Quote: ... 5′-CCCAAGACTTCCACCTACATTC -3′ (Tm = 62°C) and subsequently subcloned into PCR II-TOPO-TA cloning vector (Invitrogen). Riboprobes were generated with a BIOT-NTP labeling kit (Roche) ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... The aRNA was fragmented by incubating 3 min at 70 °C in Zn2+ RNA fragmentation reagents (Ambion) and purified with 2× volumes of SPRI beads ...
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bioRxiv - Microbiology 2021Quote: ... for 3 hours at 37 °C before transforming 5 μL into TOP 10 chemically-competent bacteria (Invitrogen). Mutations were confirmed in selected clones by DNA sequencing ...
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bioRxiv - Immunology 2023Quote: ... Amplified RNA was fragmented by incubation at 70°C for 3’ in Zn2+ RNA fragmentation reagent (Ambion). RNA was then ligated to the MARSseq ligation adaptor with T4 RNA Ligase I (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL formulated MS-Hu6 was incubated at –80 °C for 3 hours (TSX series freezer, ThermoFisher). Samples were thawed for 30 minutes at 25 °C in an Isotemp 250 thermostat (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: DRG neurons were loaded at 37°C with 3 μM Fura-2AM (Cat#F-1221, Life technologies, stock solution prepared at 1 mM in DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... Spike-in controls cel-miR-39-3p (C. elegans sequence: 5’-UCACCGGGUGUAAAUCAGCUUG-3’, Invitrogen, Waltham, MA, USA) and cel-miR-54-3p (C ...
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bioRxiv - Developmental Biology 2024Quote: Reverse transcription was carried out at 42°C for 3 hours with Superscript II (Invitrogen, 18064-014). The reaction mix included 10 U/ml reverse transcriptase ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with antibodies against eGFP (Invitrogen, A6455, 1:1000) or mHTT protein (1-82aa ...
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bioRxiv - Neuroscience 2022Quote: ... CRTD1-NLS-EGFP hiPSCs were dissociated by TrypLE Express (ThermoFisher Scientific), resuspended in cold mTeSR Plus medium with CloneR and immediately sorted using a FACS Aria III (BD) ...
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bioRxiv - Molecular Biology 2022Quote: ... and EGFP cDNA was cloned into pENTR1A (Thermo Fisher Scientific Inc.) vector ...
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bioRxiv - Microbiology 2024Quote: ... PClneo-EGFP incorporation was investigated by quantitative PCR (ABI7500, Applied Biosystems) using a plasmid standard to determine the number of pseudogenomes ...
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bioRxiv - Immunology 2024Quote: ... containing eGFP and puromycin using Phusion™ (ThermoFisher Scientific, Waltham, Ma) PCR and Gibson Assembly® Cloning Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... and chicken anti-eGFP (to detect eYFP, 1:1000; A10262 Invitrogen) in the blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen: 11668019). 18 to 24 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019, Invitrogen). 18–24 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immunostained with primary anti-EGFP (1:1000, Invitrogen, A10262) and secondary anti-chicken Alexa 488 (1:800 ...
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bioRxiv - Cell Biology 2024Quote: ... were transferred into pCMV-DEST- EGFP using Gateway® recombination (Invitrogen) (11) ...
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bioRxiv - Cell Biology 2024Quote: ... LifeAct-eGFP expressing DCs labeled with Hoechst (NucBlueTM, Hoechst 33342, Invitrogen) were imaged with an inverted widefield Nikon TiE2 microscope equipped with 20x/0.5 NA PH1 air objective using a Hamamatsu EMCCD C9100 camera and a Lumencor Spectra X light source (390 nm ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Bioengineering 2022Quote: Constructs were incubated for 1 hour at 37°C with 3 mM Fluo3 AM calcium indicator (ThermoFisher Scientific) in differentiation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 sec 72 °C) with fluorescent readings during annealing and elongation on a QuantStudio 3 machine (Thermo Fisher). Three reference genes were tested ...
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bioRxiv - Bioengineering 2020Quote: ... hBMSCs (passage 3) were expanded at 37°C and 5% CO2 in expansion medium (Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Tail and ear fragments from C57/B6 or mT/mG mice were dissected and incubated in 2-3 hours at 37 °C in 950 µL of Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10% inactivated fetal bovine serum (FBSi ...