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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were incubated for 3 h at 37 °C in CRB with tBOC-Leu-Met-CMAC (25 µM; A6520; Thermo Fisher Scientific, OR, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Neuroscience 2024Quote: ... and diluted secondary antibodies were applied at 4 °C for approximately 3 hours using Alexa Fluor 488 goat anti-rabbit (1:1000; Invitrogen, Waltham, MA; Catalog #A11008); Alexa Fluor Plus 555 goat anti-rat (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... were determined by incubating 10 μL of 10× dilutions onto 7H10 agar plates at 37 °C for 3 days (Fisherbrand Isotemp 500 series oven; Fisher Scientific, Ottawa, Ontario, Canada).
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
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bioRxiv - Microbiology 2020Quote: ... and their derivatives were grown at 37°C (or 28°C when the temperature-sensitive pMBsacB plasmid was present and extrachromosomal) under stationary conditions in tryptic soy (TS) medium (Fisher Scientific cat. # DF0370-17-3), supplemented with 5 µg/ml erythromycin ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed with PBS and finally were enzymatically digested for 1 hr at 37° C in a solution of Collagenase (3 mg/mL; Life Technologies BV, Zug ZG, Switzerland) and Dispase (4 mg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Cell Biology 2023Quote: ... from the trapping column onto an Acclaim Pepmap100 C18 column (3 µm particles, 75 μm × 500 mm, 40°C; Thermo Fisher Scientific, Waltham, MA, USA) by 50 min nonlinear gradient program (1-56% of mobile phase B ...
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bioRxiv - Cell Biology 2021Quote: WT and Vim-/- MEFs are transfected with an EGFP-actin plasmid using Lipofectamine 2000 transfection agent (Invitrogen) and imaged on the third day ...
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bioRxiv - Neuroscience 2020Quote: GFP reporter plasmids (eGFP, PLL3.7 shRNA) were transfected into neurons at DIV 14 using Lipofectamine 2000 (Invitrogen). LNA Gapmers targeting ADEPTR or siRNAs targeting kinesins were transfected using Lipofectamine RNAiMax (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... ARPE-1 (RPE) cells stably expressing clathrin light chain-eGFP were grown in DMEM/F12 medium (Gibco) supplemented with 10% FBS [74] ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the A2Lox.Cre_TRE-Nkx2-1/Pax8_Tg-EGFP mouse ESCs (Romitti, 2021) were transfected using Lipofectamine 3000 reagent (Invitrogen), and after 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293-based EGFP reporter and Hela cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... MEFs co-expressing FHL2-eGFP and Lifeact-mApple were incubated with 5 μg/mL Hoechst (Invitrogen, H1399) for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: Antibody staining for eGFP (GFP Rabbit Polyclonal #A11122 or GFP Chicken Polyclonal #A10262, Invitrogen, Thermo Fisher, USA) and/or mCherry (Living Colors DsRed Polyclonal Antibody #632496 ...
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bioRxiv - Developmental Biology 2022Quote: Antibody staining for eGFP (GFP Rabbit Polyclonal #A11122 or GFP Chicken Polyclonal #A10262, Invitrogen, Thermo Fisher, USA) and/or mCherry (Living Colors DsRed Polyclonal Antibody #632496 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in rabbit antiserum against eGFP or mCherry (1:2000; ThermoFisher Scientific, MA, USA) in a PB solution containing 2% NHS and 0.2% Triton X-10 (48 hours at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS cells expressing LAMP1-EGFP were incubated in 1X PBS with Lysotracker DND99 (Invitrogen, L7528, 50 nM) for 5 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... COX8-EGFP-mCherry-expressing U2OS cells were then selected with 800 µg/ml of G418 sulfate (Gibco). An expression vector encoding for BNIP3 fused to mCherry (pmCherry C1-BNIP3 ...
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bioRxiv - Immunology 2024Quote: ... eGFP was observed at 24 hours post transfection (HPT) using an EVOS M5000 (ThermoFisher Scientific, Waltham, MA). eGFP positive ...
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bioRxiv - Microbiology 2023Quote: The EILV-eGFP and mock-infected C6/36 cells were detached using trypsin (Gibco/Thermo Fisher Scientific) after the 48 h incubation and seeded in a 24-well tissue culture plate (Greiner Bio-One ...
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bioRxiv - Microbiology 2023Quote: The EILV-eGFP and mock-infected C6/36 cells were detached using trypsin (Gibco/Thermo Fisher Scientific) after the 48 h incubation and seeded in a 24-well tissue culture plate (Greiner Bio-One ...
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bioRxiv - Microbiology 2023Quote: ... The virus mixture was prepared by combining 0.3 mL of VSVΔG-EGFP-Gp P3 (titer ∼10e8/mL) and 1.7 mL of DMEM (Gibco) supplemented with 8% FBS (Hyclone ...
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bioRxiv - Immunology 2023Quote: Overnight-rested mouse naïve CD4+ T cells constitutively expressing LifeAct-eGFP were labeled with CellMask Orange (ThermoFisher) to stain the plasma membrane following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... mCherry and eGFP expression was visualized using a 4-channel EVOS M5000 fluorescence microscope (Thermo Fisher Scientific). Cells were harvested by trypsinization ...
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bioRxiv - Cancer Biology 2023Quote: ... cells expressing active or inactive mRuby-Grubraw and EGFP-Grubraw were stained with MitoTracker Green (ThermoFisher Scientific) or20 nM TMRM ...
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bioRxiv - Cell Biology 2024Quote: ... Two dishes each were transfected using the manufacturer’s protocol with either EGFP-p130cas (25µg plasmid DNA, 75µl Lipfectamine 2000 (Invitrogen)) or tagRFP-paxillin (25µg plasmid DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and the EGFP and Spike loci were PCR-amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific). Amplicons were indexed by PCR using Nextera indexes (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... human MLX ( accession number NM_170607.3), and eGFP coding sequences were synthesized by Genewiz (Suzhou, China) and cloned into pcDNA3.1 vectors (Invitrogen). GK was subcloned into pcDNA3.1 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... 2000 to 3000 EGFP+ CAFs were collected from each tumor in 500 µl of DMEM (Gibco, 11995065) supplemented with 10% FBS (Foundation C ...
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bioRxiv - Cell Biology 2024Quote: ... and rtTA (phr_tet3g) or pLV-TetO-hNGN2-eGFP-Puro using OptiMEM and Lipofectamine 2000 (Fisher Scientific 11668019). On the day of transduction (48 hours later) ...
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bioRxiv - Developmental Biology 2024Quote: ... The CCDC112-EGFP vector was then transfected into IMCD3 cells using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: 80 eGFP-positive embryos were incubated in a 5 mL solution of TrypLE Express (ThermoFisher Scientific, 12605036) and 0.003% Tricaine (ethyl-3-aminobenzoate-methanesulfonate ...