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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were washed once with PBS and incubated at 37°C for 1 h with ONPG solution (3 mg/mL (Fisher Scientific, 34055) + 0.05% NP-40 ...
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bioRxiv - Immunology 2020Quote: ... blood was collected postmortem via heart puncture on day two after infection (n = 3 mice/treatment) and kept at −20 °C in RNAlater (Thermo Fisher Scientific). RNA was extracted using the Mouse RiboPure Blood RNA Isolation Kit and globin RNA depleted using the GLOBINclear Kit ...
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bioRxiv - Microbiology 2022Quote: ... the seven strains were each cultured for 3 months at 27°C in 100 mL media (175 cm2 growth flasks, cat: NUN159910, Thermo Fisher Scientific) and then split into 25 cm2 flasks for the assays ...
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bioRxiv - Molecular Biology 2022Quote: ... SP3 protein binding occurred for 30 min at room temperature and beads were subsequently washed 3 times with 80% ethanol for resuspended with 175 ul of Trypsin/Lys-C (1 µg, Thermo Scientific A40009) in 200 mM EPPS (pH 8.4)/5 mM CaCl2 ...
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bioRxiv - Plant Biology 2022Quote: ... from the trapping column onto an analytical column (Acclaim Pepmap100 C18, 3 µm particles, 75 μm × 500 mm; column compartment temperature of 40 °C, Thermo Fisher Scientific) by 135 min nonlinear gradient program (1-56% of mobile phase B ...
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bioRxiv - Neuroscience 2024Quote: ... The LC/MS analysis was performed using a Thermo Scientific Dionex UltiMate 3000 system connected to a PepMap C-18 trap-column (0.075 × 50 mm, 3 μm particle size, 100 Å pore size; Thermo Fisher Scientific) and an in-house-packed C18 column (column material ...
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bioRxiv - Immunology 2024Quote: ... for 13 hours at 37 °C and 5% CO2 in the presence of Brefeldin A (3 µg/mL; ThermoFisher; Waltham, MA, USA) for the last 10 hours of stimulation ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then centrifuged at 1000rpm for 3 minutes at 4°C before being suspended in 100μl HBSS (ThermoFisher, Cat No. 14025) + 1% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Both Brca2+/+ and Brca2-/- cell lines were cultured under low oxygen conditions (3% O2, 5% CO2, 37°C) using DMEM (GIBCO; 41966-029) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The oligonucleotides 5’-cac cgA GCC AAG TCT GTT ATG TGC A-3’ and 5’-aaa cTG CAC ATA ACA GAC TTG GCT c-3’ were annealed and then ligated into the vector using T4 PNK and T4 ligase (Thermo Fisher Scientific). One Shot competent cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies were incubated on slides in a the dark for 3 hours at room temperature or overnight at 4°C: GαM 488 (Invitrogen A21121, 1:1000), GαRb 546 (Invitrogen A11010 ...
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bioRxiv - Bioengineering 2023Quote: ... extension: 68 °C for 42 secs and total number of cycles: 35) in ProFlex 3×32-well PCR system (Applied Biosystems, USA). Desired amplified product was excised from 1.2% agarose gel and purified using GeneJET gel extraction and DNA cleanup kit following supplier’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... before being spun (5000rpm 4⁰C for 3 minutes) and homogenised (70 seconds with a pestle) in 300 ul trizol (Invitrogen 15596-026). RNA was extracted using the Zymo Direct-zol RNA Microprep Kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... Mobile phase A was ultrapure water of ASTM I type (resistance 18.2 MΩ.cm at 25°C) prepared by Barnstead Smart2Pure 3 UV/UF apparatus (Thermo Fisher Scientific, Bremen, Germany) with 0.1 % (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Neuroscience 2024Quote: ... Mobile phase A was ultrapure water of ASTM I type (resistance 18.2 MΩ.cm at 25°C) prepared by Barnstead Smart2Pure 3 UV/UF apparatus (Thermo Fisher Scientific, Bremen, Germany) with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were derived from KB1P mammary tumors as previously described29 and were grown at 37° C in 3% O2 in DMEM Nutrient mixture F-12 (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific, EA0375PK2). Gels were run at 80V for 3 h and transferred onto a PVDF membrane in Tris-Glycine buler for 90 min at 90V ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% non-fat milk for 3 hours and incubated overnight at 4°C with primary antibodies against COL1 (1:1000, Invitrogen, PA5-95137), COL3 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were incubated at 4°C for 3 days in PBS containing Alexa Fluor 488–conjugated isolectin GS-IB4 (Griffonia simplicifolia, 2 μg/ml, Invitrogen, Rockford, IL) and anti-laminin primary antibody (LAMA2 ...
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bioRxiv - Cell Biology 2024Quote: ... and counted to yield 3×105 cells per well cultured in suspension on an orbital shaker at 37°C with StemScale PSC medium (Thermo Fisher Scientific) supplemented with 10 µM Y27632 (PeproTech) ...
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bioRxiv - Synthetic Biology 2024Quote: ... of 3-4 months based on previous established methods188 and maintained at 37°C and 5% CO2 in DMEM (ThermoFisher, MA, USA), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were incubated for 3 h at 4°C under gentle rotation with Pierce Protein G Plus Agarose (Thermo Fisher Scientific) conjugated to antibodies specific for FUS (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... were then blotted for 3 to 6 s at 100% humidity (4 °C) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted superresolution bin 2 mode on a Titan Krios G3 (FEI ...
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bioRxiv - Biochemistry 2024Quote: ... it was centrifuged 3-5x at 1,300 x g (5 min, 4 °C, Heraeus multifuge X3R, Thermo Scientific, rotor F15-8×50cy) until no pellet was visible ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was then probed with specific primary antibodies diluted 1:1000 in antibody dilution buffer (3% BSA in TBS-T) and incubated overnight at 4°C - either anti-TNFR1 rabbit monoclonal antibody (Invitrogen, #MA5-14899) or anti-β-Actin mouse monoclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at room temperature (RT) prior to overnight incubation at 4 °C with one of the following primary antibodies: Caspase 3 (Invitrogen, Cat# 700182), Insulin (SCBT ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Td-TASK2/TALK1 and Td-TASK2-TALK2 were subcloned into the pIRES2-EGFP vector (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: CasRx-eGFP from pXR001 vector was PCR amplified and cloned into pVax1 (ThermoFisher Scientific, USA) by restriction enzyme cloning ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Biophysics 2020Quote: ... which was generated by sequentially inserting SmF and EGFP or mCherry into pRSF-Duet1 (Invitrogen) with an N-terminal His6 tag ...
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bioRxiv - Neuroscience 2020Quote: ... E3 or E4 cDNA into the p-EGFP-N1 or p-mCherry-N1 vector (Invitrogen) between the XhoI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... We incubated the remaining filtrate with anti-EGFP-coupled protein G Dynabeads (Invitrogen, cat. #10003D) for 1 hour at 4°C with gentle end-over-end rotation ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M proteins were probed using rabbit anti-EGFP polyclonal antibody (1:1000) (Invitrogen #A6455). The Venus fluorescent protein used in BiFC experiments is a genetic variant of EGFP ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Jurkat-E6-LTR-Tat-EGFP cell lines were grown in RPMI 1640 medium (Gibco) supplemented with 10 % FCS and 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... and the filtrate was incubated with anti-EGFP-coupled protein G Dynabeads (Invitrogen, cat. #10003D) for 1 hour at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... MS2-eGFP HeLa cells were then transfected using JetPrime™ (Life Technologies, Saint Aubin, France) with a mixture of plasmids encoding for pIntro ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were subjected to immunostaining against EGFP using anti-GFP antibody (1/500, A11122, Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-GFP (used for EGFP, citrine, EYFP; 1:1000; Thermo Fisher Scientific, A-11122); goat anti-GFP (used for EGFP ...
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bioRxiv - Cell Biology 2020Quote: ... or pWPI-eGFP-NDI138 constructs with the with the Lipofectamine® 2000 transfection reagent (Invitrogen) with 1.6 μg plasmid DNA according to manufacturer instruction 48 h prior to passaging and galactose culture.
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bioRxiv - Developmental Biology 2020Quote: ... Sorted EGFP-positive cells were collected in HBSS without Ca2+ and Mg2+ (ThermoFisher, 14170-112) containing 1% FBS and 0.4% BSA ...
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bioRxiv - Cell Biology 2021Quote: Cardiac fibroblasts from RosaCas9-EGFP mice were transfected with guide RNAs using Lipofectamine MessengerMAX (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the EGFP-CRAF-HaloTag expression vector using Lipofectamine3000 (Thermo Fisher Scientific Inc.). Concentrations of cDNA and P3000 reagent were reduced to 10%–20% of those for the standard protocol and incubation time was shortened to 3 h from 2–4 days recommended for the standard protocol ...
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bioRxiv - Bioengineering 2022Quote: ... then co-transfected with plasmids pRK5-EGFP-tau and an empty vector pUC-18 (ThermoFisher) to equalize the amount of plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with Kif1A-tdTomato and EGFP-FRB-EEA1 using Lipofectamine 2000 (ThermoFisher, Cat #11668027). The following day ...
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bioRxiv - Cell Biology 2022Quote: ... and transfected with Caveolin1-EGFP plasmid (gift of Richard Lundmark) using Lipofectamine3000 (Invitrogen, L3000-001). After 48 h the cells were washed with PBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Staining for F-actin was done using eGFP-conjugated phalloidin (Life Technologies R37110, 1:1000).
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bioRxiv - Neuroscience 2023Quote: ... was subjected to immunoprecipitation using anti-EGFP antibodies conjugated to Protein G magnetic Dynabeads (Invitrogen). The beads were washed ...