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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... RNP-EGFP solution was transferred to Lipofectamine CRISPRMAX Cas9 Transfection Reagent (ThermoFisher, CMAX00001) according to manufacturer’s instructions and applied to 6-well plates in drop-wise fashion ...
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bioRxiv - Biochemistry 2024Quote: ... Tom70-EGFP-CNPase-ALFAtag was ordered as a synthetic gene (GeneArt Thermo Scientific) and cloned into the mammalian expression plasmid pcDNA3.1 using Gibson assembly (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... for 48-72 h (chicken anti-eGFP (1:2000, Thermo Fisher Scientific, A10262) and/or Alexa 594-conjugated streptavidin (1:500 ...
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bioRxiv - Cell Biology 2024Quote: Imaging of LifeAct-eGFP expressing cells labeled with Hoescht (NucBlueTM, Hoechst 33342, Invitrogen) was performed under physiological conditions using custom-built climate chambers (37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... HPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 µm, operated at 40° C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Biophysics 2021Quote: ... Grids were blotted for 3 s with a blotting force of 3 and 100% humidity at 22 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg/ml in sterile water Gibco 10977035, 1 hour at 37°C followed by 3 washes with PBS Gibco 10010015) / Laminin (Gibco 23017-015 ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were collected by centrifugation (200 g, 3 min at 25 °C) and resuspended in complete medium comprising neurobasal medium (Gibco, 21103) supplemented with 2% B-27 plus (Gibco A3582801) ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the RNA samples were fragmented with FastAP buffer at 94 °C for 3 min and were dephoshorylated using the FastAP enzyme (Thermo Scientific) at 37 °C for 30 min followed by bead purification with 2 volumes of Agencourt RNAClean XP beads ...
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bioRxiv - Biophysics 2021Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Microbiology 2020Quote: ... of 0.001-0.01 and amplified for 1-3 hours at 37 °C at 250 rotations per minute (rpm) (MaxQ4000 shaker, Thermo Fisher Scientific) until lysis was observed ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 cycle at 72°C for 3 min using a Veriti Thermal Cycler (Applied Biosystems, Foster City, CA, USA). The PCR products were analyzed by heteroduplex mobility assay using microfluidic capillary electrophoresis system caliper LabChip GX (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... 3 μm C 18 beads (Nikkyo Technologies, NTCC-360/75-3-123°Column) with buffer A: 0.1% formic acid (Fisher Scientific, A11750), and buffer B ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... HPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 × 250 mm, 3 μm, operated at 50°C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC system(Agilent ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... HPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 × 250 mm, 3 μm, operated at 50°C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... after denaturation by 70°C for 3 minutes and separated by gel electrophoresis using 10% TBE-Urea gels (Invitrogen, Cat# EC68755BOX) at 180V for 60 min in 1x TBE electrophoresis buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... 15N-labeled seedlings were then dried for 2-3 days at 65°C and further analyzed by Thermo Finnigan Delta plus XP IRMS (ThermoFisher Scientific). For primary root length determination ...
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bioRxiv - Cancer Biology 2020Quote: ... XmaI+ClaI.SV40-pA 5’-ATG CAT GCA TCC CGG GCA TCG ATG CAG TGA AAA AAA TGC TTT ATT TGT GAA ATT TGT GAT GCT ATT GCT TTA TTT GTA ACC-3’ and SV40-pA.EcoRI+XbaI 5’-ATG CAT GCA TGA ATT CGT CTA GAA ACT TGT TTA TTG CAG CTT ATA ATG GTT ACA AAT AAA GCA ATA GCA TCA C-3’ and PCR SuperMix Taq (Invitrogen #10572014), we amplified a 128 bp fragment (5’-ATG CAT GCA TCC CGG GCA TCG ATG CAG TGA AAA AAA TGC TTT ATT TGT GAA ATT TGT GAT GCT ATT GCT TTA TTT GTA ACC ATT ATA AGC TGC AAT AAA CAA GTT TCT AGA CGA ATT CAT GCA TGC AT-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Immunology 2021Quote: ... 200,000 cells from each B cell subset were sorted into pure FBS on ice and immediately washed 3 times in 37°C PBS and labeled with CellTrace carboxyfluorescein succinimidyl ester (CFSE) Cell Proliferation Kit (Invitrogen; 1µM) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the chromatin and the 10% input were both reverse-crosslinked at 65°C for 3 hours with 10 μg of RNase A (ThermoFisher, EN0531) and 20 μg of thermoresistant proteinase K (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Cell Biology 2023Quote: The HPLC run was performed using a C30 reverse-phase column (Thermo Acclaim C30, 2.1 250 mm, 3 mm, operated at 40°C; Thermo Fisher Scientific) connected to a Shimadzu LC-20AD HPLC system ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... UHPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1x 250 mm, 3 μM, operated at 55° C; Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 UHPLC system and a Q-Exactive Orbitrap high resolution mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... UHPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 μm operated at 55° C; Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 HPLC system and a QExactive orbitrap MS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Biochemistry 2023Quote: ... A temperature ramp from 5°C to 95°C was set up at a speed of 0.02°C/s on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). All measurements were performed in triplicates ...
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bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...