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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... after agarose gel electrophoresis and blunt end cloning was performed into pCR™-Blunt II-TOPO™ Vector (Invitrogen). Site-directed mutagenesis was performed on plasmid that carried E gene to introduce the desired mutation using primer pairs (Table S2) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Immunology 2024Quote: ... and uterine tissue lysates (75 μg per well) were subjected to electrophoresis in sulfate-polyacrylamide gels (Cat# NP0336BOX; Invitrogen). The proteins were transferred onto nitrocellulose membranes (Cat# 1620145 ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA pH 8.0 and then labeled with the ULYSIS™ Alexa Fluor™ 594 nucleic acid labeling kit (Thermo Fisher Scientific, MA, USA). Two μg of either an equimolar mixture of the eleven Altivir_1_MSI polynucleotides or of the single negative control polynucleotide were used in a single labeling reaction and then purified using NucAway Spin Columns (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
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bioRxiv - Biophysics 2019Quote: ... The pellet has been finally resuspended in nuclease free water and the amount of nucleic acid determined through NanoDrop technology (ND 1000, Thermo Fisher Scientific, Waltham, MA, USA). Ten ng of total RNA was subjected to reverse-transcription with the miRCURY LNA miRNA PCR System (QIAGEN ...
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bioRxiv - Genomics 2021Quote: ... Spores were crushed with a sterile pestle after adding 180 µl of 1x PBS and DNA was stained by adding 1 µl of 200x SYBR Green I Nucleic Acid stain (Invitrogen™, Thermo Fisher Scientific, MA, USA). The nuclear sorting with flow activated cell sorter (FACS ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of eluted anterior nares specimens were extracted on the KingFisher Flex using the MagMAX Viral Pathogen II Nucleic Acid Isolation Kit with MagMAX™ Viral/Pathogen Ultra Enzyme Mix (Thermo Fisher A48383 and A42366) and eluted in 50 μL (although ~35 μL is eluted).
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bioRxiv - Pharmacology and Toxicology 2022Quote: HBV DNA was extracted from 5 μL of serum using the SMITEST EX-R&D Nucleic Acid Extraction Kit (Medical & Biological Laboratories, CO. LTD, Nagoya, Japan) and dissolved in 20 μL nuclease-free water (Thermo Fisher Scientific Inc., Waltham, MA). Real-time PCR was used to measure the serum HBV DNA concentration using the TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Media was removed from RPE1 cells and replaced with warmed 1x HBSS containing 1:500 dilutions of both Sytox Green nucleic acid stain (Life Technologies, S7020, 1:500 dilution) and Hoechst (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... Two hundred milliliters of diluted plasma sample were used for nucleic acid extraction using MagMAX™ Pathogen RNA/DNA Kit (Thermo Fisher Scientific, MA, USA). The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification ...
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bioRxiv - Plant Biology 2020Quote: ... We controlled the RNA integrity through agarose gel electrophoresis (1.5 %) and RNA concentration and purity were measured with a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... equal protein amounts were loaded and then separated by electrophoresis through NuPAGE Novex Bis-Tris 4–12% gradient gels (Invitrogen). Proteins were transferred onto Nitrocellulose membranes using the iBlot system and protocol from InVitrogen (Carlsbad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific, cat# 26619) molecular weight markers ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified fragments were resolved in agarose 0.8% (w/v) gel electrophoresis together with 1 Kb Plus Ladder (Invitrogen, 10787026). While vsp1 ...
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bioRxiv - Microbiology 2019Quote: ... Surveyor assay was performed using Sarm1AG3S PCR conditions and Surveyor Mutation Detection Kit (IDT) followed by electrophoresis on Novex 20% TBE gels (Invitrogen). Off-target CRISPR cleavage was accessed by PCR amplification using the primers listed in table I and cycling conditions 95° 30 sec ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen) at 225 V for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10%) and then transferred to nitrocellulose membranes (Thermo Fisher). The membranes were first blocked using 5% non-fat milk ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA quality and concentration were determined by denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... were separated by electrophoresis through 10% (for nuclease knockdown and overexpression experiments) or 15% (for dual reporter experiments) TBE-urea gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were heated to 95°C for 5 minutes prior to electrophoresis through a 12% Bis-Tris precast gel (Invitrogen), followed by transfer to a nitrocellulose membrane by wet blotting ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were visualized by electrophoresis on a 1% agarose and 1X TAE gel containing 0.3 μg/mL ethidium bromide (Invitrogen, 15585011). Gel extraction was performed to select for products between 200 – 1000 bp using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2021Quote: The purified DNA fragments separated by electrophoresis in 1% agarose gel and transferred by the alkaline capillarity method to the Bio-dyne nylon membrane (Invitrogen) in a denaturing solution (NaOH 0.4 M) ...
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bioRxiv - Microbiology 2021Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a Qubit® fluorometer (Thermo Fisher Scientific, Waltham, MA). Library construction and sequencing was based on the 96-plex genotyping-by-sequencing (GBS ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were size-selected (50-400 bp) using agarose gel electrophoresis and quantified fluorometrically using a Qubit 3.0 Fluorometer (Life technologies), and a total of 10 ng of digested DNA (200 pg l-1 ...
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bioRxiv - Microbiology 2021Quote: ... the extracted DNA samples were checked for quality and quantity by a 1% agarose gel (Midori Green-stained) electrophoresis and a Qubit dsDNA HS Assay Kit (Invitrogen). Prior to library preparation ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were separated by electrophoresis on a 1.5% agarose gel and stained with SYBR® safe (Thermo Fisher Scientific) before being visualized under an ultraviolet transilluminator ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments resulting from nuclease activity during the Surveyor assay were separated by electrophoresis in 10% TBE-polyacrylamide gels (ThermoFisher). A quantitative measurement of optical density through Image J was performed to quantify efficiency of CRISPR/Cas9 editing ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were analyzed by 1% agarose gel electrophoresis and visualized under UV illumination after SYBR-Safe staining (Invitrogen, S33102). For Sanger sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and cloned in pCRTM4Blunt-TOPO® Vector (Invitrogen cat # K2875-20) by TA cloning ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA quality was assessed by denaturing agarose gel electrophoresis and quantified by the Qubit RNA BR Assay Kit (ThermoFisher). For library preparation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentration was measured by gel electrophoresis (5µl sample on 1.2% agarose) and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Biophysics 2022Quote: SDS-PAGE electrophoresis was conducted by using Novex® NuPAGE® SDS-PAGE Gel System (Life Technologies, Carlsbad, CA, USA). NuPAGE® 4–12% Bis-Tris gels (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen). Proteins were transferred onto polyvinylidene fluoride membranes (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The quality of the DNA was assessed by gel electrophoresis and the quantity was estimated using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) and a Tecan Infinite 200 Microplate Reader (Tecan Deutschland GmbH).
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bioRxiv - Microbiology 2021Quote: ... and was detected by the agarose gel electrophoresis as well as quantified by Qubit® 2.0 (Thermo Scientific, Waltham, USA). Libraries for ONT sequencing were prepared using an SQK-LSK109 kit of Oxford Nanopore Technologies Company ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 30 μg of protein was subjected to gel electrophoresis and transferred to membrane using Bolt western blot reagents from ThermoFisher Scientific Inc (bolt 4-12% Bis Tris gel ...
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bioRxiv - Immunology 2020Quote: ... Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 2 hours using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... RNA quality was determined via agarose gel electrophoresis and RNA purity and quantity was measured via NanoDrop 2000C (Thermo Scientific). 1000µg total RNA was used in iScript cDNA Synthesis (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 µl of cell lysates were separated on 4-12% tris-glycine SDS-polyacrylamide gel electrophoresis (SDS-PAGE) from Invitrogen, and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2020Quote: ... 1μL aliquots were analysed by non-reducing SDS-PAGE electrophoresis on NuPAGE™ 12% Bis-Tris protein gels (ThermoFisher Scientific) to confirm the presence of non-aggregated HLA-E heavy chain and β2m.
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bioRxiv - Developmental Biology 2022Quote: ... The quality of the gRNA prep was confirmed by agarose gel electrophoresis and quantified using a NanoDrop One (Thermo Fisher).
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bioRxiv - Plant Biology 2019Quote: Blue native polyacrylamide gel electrophoresis (BN-PAGE) was performed using the Bis-Tris NativePAGE system from Invitrogen (Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA quality was assessed using a NanoDrop (ThermoScientific) and by electrophoresis on 2% agarose gel containing ethidium bromide (Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... The quality and quantity of the DNA was checked using gel electrophoresis and spectrophotometric analysis (NanoDrop 1000, Thermo Scientific, USA).
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bioRxiv - Developmental Biology 2019Quote: ... We analyzed protein samples by electrophoresis on 4%–12% NuPAGE SDS-PAGE gels with MES SDS Running buffer (Life Technologies) and corresponding LDS buffer NuPage (Invitrogen ...