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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Pooled samples of protein extracts (80 μg) were separated using electrophoresis on 4– 12% NuPage Bis-Tris gels with MOPS buffer (Invitrogen, Carlsbad, CA) and then transferred to Immobilon-P membranes (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific). The membranes were blocked in 5% nonfat milk (RPI International ...
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bioRxiv - Genomics 2023Quote: All PCR products were assessed by gel electrophoresis and quantified using the Qubit™ dsDNA HS Assay kit (Cat. #Q32854; Thermo-Fisher Scientific). Primers were synthesized by Integrated DNA Technologies® and diluted to 10uM in 0.1x TE buffer ...
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bioRxiv - Biophysics 2023Quote: ... in the presence of different concentrations of uranyl acetate (0–1000 µM) were analyzed with blue native polyacrylamide gel electrophoresis (BN- PAGE) using the Invitrogen system (ThermoFisher Scientific, USA). Thus ...
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bioRxiv - Cell Biology 2023Quote: ... or 10μg EV proteins were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis (10-12% polyacrylamide, SureCast™ Acrylamide Solution, ThermoFisher Scientific, MA) and transferred onto PVDF membranes ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of isolated RNA samples were assessed by performing 1% agarose gel electrophoresis and NanoDrop 2000C (Thermo Fisher, USA). The high-quality RNA samples were outsourced for RNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were transferred into fresh tubes and 50 µg of each sample was subjected to native gel electrophoresis to reveal the various proteasome complexes (30S, 26S, 20S) using a NativePAGE™ 3 to 12% Bis-Tris gel (Invitrogen, BN1001BOX) in XCell™ SureLock™ Mini-Cell system (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The purity of viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (NovexTM 4-12% Tris-Glycine, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: Protein blot analysis was performed following electrophoresis of reduced samples using NuPAGE 4 to 12% Bis-Tris Mini Polyacrylamide Gels (Thermo Scientific, #NP0322BOX). Protein was then transferred to PVDF membranes using the iBlot 2 Dry Blotting System from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and their purity validated by sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blot (WesternBreeze Chromogenic Immunodetection System, Thermo Fisher Scientific) using antibodies against His6xtag.
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Immunology 2023Quote: ... to amplify immunoglobulin heavy and light chain variable regions and yielding variable regions were confirmed by using 96 well E-gel agarose electrophoresis (Thermo Fisher Scientific). Purified PCR amplicons were then sequenced with forward and reverse primers and contigs of the amplified immunoglobulin sequences were made by the Duke automated sequence analysis pipeline (Duke ASAP) ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity of all newly synthesized RNAs was detected using UREA-polyacrylamide gel electrophoresis (PAGE) which was stained with SYBR™ Gold (Invitrogen, S11494). The gels were finally visualized on a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... Capillary electrophoresis (Applied Biosystems 3500 XL) was used to probe the remaining fluorescently labeled DNA products ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was either amplified by PCR and detected by gel electrophoresis or quantified by qRT-PCR using the PowerUp SYBR green qPCR kit (Applied Biosystems, catalog # A25918).
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bioRxiv - Neuroscience 2021Quote: ... The concentrated RNA (10-15 µg per sample) was then size fractionated by electrophoresis using 15% polyacrylamide-urea gels (ThermoFisher Scientific, catalog # EC68852BOX) run at 200 volts in TBE (89 mM Tris-borate ...
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bioRxiv - Molecular Biology 2020Quote: Western blot analysis was carried out using standard procedures for SDS-PAGE electrophoresis on 4-12% NuPage gels in MOPS buffer (Invitrogen, Carlsbad, CA, USA), then transferred to nitrocellulose membranes (Whatman ...
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bioRxiv - Genetics 2020Quote: Proteins eluted from the beads were separated by SDS/PAGE electrophoresis on a 10% polyacrylamide gel and stained with Colloidal blue silver (ThermoFisher Scientific, USA; 70). SDS-PAGE gel lanes were cut into 2-mm slices and subjected to in-gel reduction with dithiothreitol ...
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bioRxiv - Developmental Biology 2020Quote: ... Purity of eluted gRNA product was determined by formalin gel electrophoresis and gRNA was quantified on a Nanodrop 2000c (ThermoFisher Scientific, Waltham, MA). Following quantification ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... the input DNA quality was verified using gel electrophoresis and the quantity was measured using the Picogreen assay (Thermo Scientific, Waltham, MA, USA). 2ng of DNA was prepared in 30ul of buffer and used for library construction using the Nextera DNA Flex Library Kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA quality was evaluated with electrophoresis in a 1% (w/v) agarose gel and DNA concentrations were evaluated using Qubit (Thermo Fisher Scientific, USA). An equal amount of DNA from each sample was used for library preparation with the NEBNext DNA Ultra2 Library Preparation Kit (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a NanoDrop spectrophotometer ND-1000 version (Thermo Fisher Scientific, Wilmington, DE, USA). For GBS library construction ...
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bioRxiv - Microbiology 2020Quote: ... DNA concentration and purity were determined by agarose gel electrophoresis and fluorometrically using RiboGreen (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a Nano Drop® ND-2000 spectrophotometer (Thermo Scientific Inc., USA) and kept at −80 ℃ prior to further use ...
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bioRxiv - Plant Biology 2019Quote: ... All PCR-amplified fragments were analyzed by taking 10 μL of reaction and run on an electrophoresis gel of 1.5% agarose (Life Technologies, Carlsbad, CA, USA) and 1x TBE (50 mM Tris-HCl pH 8 ...
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bioRxiv - Pathology 2021Quote: Amplicons were purified by agarose gel electrophoresis and sequenced directly using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI Prism R3500 (Applied Biosystems, Foster City, CA.). Twenty six previously published sequences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific, Dreieich, Germany). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... 10µg of protein was submitted to SDS-PAGE electrophoresis using NuPAGE Novex or Bolt 4-10% Bis-Tris gels (Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Qualitative and quantitative RNA assessment of all samples was done by gel electrophoresis (1% agarose), a NanoPhotometer® P330 (Implen, Munich/Germany) and a Qubit 2.0 (Invitrogen, Carlsbad, CA/USA).
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bioRxiv - Neuroscience 2022Quote: ... The purity of produced viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were heated to 95 °C for 5 min prior to SDS-PAGE gel electrophoresis (NuPAGE MES SDS running buffer, Thermo Fisher Scientific, #NP0002). Proteins were transferred to a nitrocellulose membrane (#1704158 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019 and from callus tissues using RNeasy Plant Mini Kit (Qiagen).The quality and concentration of extracted RNA were checked with 1 % agarose gel electrophoresis and estimated with Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, USA), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified DNA fragments were resolved by electrophoresis in 1% agarose gels alongside GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific, cat# SM1331). CRISPR/Cas9-induced mutations in AS-NMD-regulated genes were characterized with primers designed to amplify either short (∼0.5 kb ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were assessed by electrophoresis on a 2% agarose gel and a Qubit 4.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). DNA sequencing libraries were prepared following standard MGI sequencing library protocol by the Beijing Genomics Institute (BGI ...
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bioRxiv - Synthetic Biology 2023Quote: ... the amplicons were separated in a 1% agarose gel after electrophoresis at 140 V and identified by size based on gene ruler Plus DNA Ladder (Thermo Fisher Scientific, Lithuania). Amplicons were purified using Favorprep™ GEL/PCR Purification Kit (Favorgen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 μl of product from 35 rounds of PCR were analyzed for each time-point in by 2% agarose gel electrophoresis (Top Vision, Thermo Scientific, Waltham, MA). Each result is representative of a minimum of two independent replicates.
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bioRxiv - Plant Biology 2023Quote: ... RNA quality was assessed by electrophoresis in agarose gel and quantified using a QubitTM RNA Broad Range kit and Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Biophysics 2023Quote: ... Purity of produced viral vectors was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific). Proteins were transferred to a polyvinylidene difluoride membrane using an XCell SureLock Mini-Cell Electrophoresis System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific, Waltham, MA). Proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates (20 μg) were analyzed via electrophoresis using 4%-12% sodium dodecyl sulphate-polyacrylamide gradient gels (Thermo Fisher Scientific, Waltham, MA, USA). Pre-stained protein ladders (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... for each sample were loaded onto 10% polyacrylamide gels and separated by electrophoresis and transferred overnight to nitrocellulose membranes (Thermo Fisher, Stockholm, Sweden). The membranes were immunoblotted with primary antibodies against actin (1:30,000 ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Microbiology 2020Quote: ... to create a gel property and brought to a pH of 5.0 using lactic acid (Fisher Scientific). The control treatment did not contain sucrose ...