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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Twenty-five µg of protein were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Fisher Scientific). Western blot analysis was performed with the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... Equivalent amounts of total protein lysate were subjected to electrophoresis on NuPAG 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and proteins were transferred to polyvinylidene difluoride membranes (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and concentration of DNA were assessed by agarose gel electrophoresis and the Qubit®3.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... the integrity of total RNA was evaluated using agarose gel electrophoresis followed by reverse transcription with Maxima H Minus First-Strand cDNA Synthesis Kit (K1682; Thermo Scientific). Quantitative RT-PCR was performed with SYBR Green regent (Takara ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (40-50µg) were denatured and separated on 12% Bis-Tris SDS–polyacrylamide electrophoresis gels (NP0341BOX, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Zoology 2023Quote: ... The RNA quality was evaluated by gel electrophoresis and absorbance determination using a NanoDrop 2000 spectrophotometer (Thermo Scientific, New York, USA). Genomic DNA was removed ...
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bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA integrity was assessed by gel electrophoresis and its concentration quantified spectrophotometrically with a NanoDrop (Thermo Fisher Scientific, Waltham MA, USA). The obtained RNA was used to inoculate groups of 4-week-old N ...
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bioRxiv - Genomics 2021Quote: ... The digest was subject to viral nucleic acid extraction using a MagMAX™ Pathogen RNA/DNA Kit on the KingFisher™ Flex Purification System (Thermo Fisher Scientific, Waltham, MA, USA) in a 96 deep-well configuration.
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bioRxiv - Evolutionary Biology 2024Quote: ... Electrophoresis was performed in an Owl™ D3-14 electrophoresis box (Thermo Scientific, MA, USA) containing 1X TBE buffer for 45 min at 130 V delivered from an Owl™ EC 300 XL power supply (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The integrity and approximate concentration of the extracted RNA was determined by electrophoresis of the RNA extracts in a 1% agarose gel containing Sybr Safe (Invitrogen, Carlsbad, CA). One microgram of total RNA was reverse transcribed to complementary DNA (cDNA ...
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bioRxiv - Biochemistry 2020Quote: Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (Thermo Fisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2021Quote: ... proteins from cell lysates were separated by 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using MOPS running buffer (Thermo Fisher, USA), and transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Genetics 2021Quote: ... Samples were heated at 100 °C for 10 min prior to electrophoresis through a Novex NuPAGE 4–12 % Bis-Tris gel (Life Technologies, # NP0335BOX) in MES SDS running buffer (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... the amplified products were visualized in 2% agarose gel electrophoresis in 1X TAE buffer and stained with SYBR Safe (Invitrogen, Waltham, MA) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... Solubilised proteins in a 25 μL volume were prepared and separated by electrophoresis on NativePAGE 3-12% Bis-Tris gels (Thermo Fisher Scientific) and NuPAGE Tris-Acetate buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR products were fractioned by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (Thermo scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ 1.47v (NIH ...
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bioRxiv - Genomics 2019Quote: ... The DNA purity was determined by 0.8% agarose gel 100 V electrophoresis for 40 min and DNA concentration was determined by Qubit® 2.0 fluorescent meter (Invitrogen, Carlsbad, USA). Following quality assessment ...
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bioRxiv - Microbiology 2020Quote: ... For gel electrophoresis we transferred 5 μl of the PCR reaction to a 1.5% agarose gel stained with SYBR Safe (Invitrogen, Thermo F. Scientific) and visualized by UV illumination ...
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bioRxiv - Genomics 2019Quote: The yield and quality of DNA were assessed by agarose gel electrophoresis and by a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, Delaware) and quantified by Qubit broad range assay (Thermo Fisher Scientific). Illumina sequencing libraries were prepared ...
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bioRxiv - Biochemistry 2019Quote: ... The purified core complex was analyzed by 10 % polyacrylamide SDS-gel electrophoresis and its purity was determined to be 80-90 % by staining with Coomassie Brilliant Blue (Thermo Fisher Scientific). The composition of the core complex was confirmed by immunoblotting with anti-Flag and anti-c-Myc antibodies ...
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bioRxiv - Genetics 2021Quote: ... The samples were returned as pellets to be re-suspended with 50 ml Elution Buffer before the DNA quality and quantity was assessed using gel electrophoresis and NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Plant Biology 2020Quote: ... The electrophoresis of amplified products was performed on 6% acrylamide gels at 160 Volts for 30 minutes (Thermo Electron Electrophoresis EC250-90, Thermo Fisher Scientific). The results were visualized under UV light using UVITEC Imaging Systems (Cleaver Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 40 µg of total RNA isolated from mESC were separated with agarose electrophoresis with RNA Gel Loading Dye (2X) (Thermo, R0641) and NorthernMax™ 10X Running Buffer (Ambion, AM8671). The separated total RNA samples were further transblotted to positively charged nylon transfer membrane (Cat ...
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bioRxiv - Genomics 2019Quote: ... Total RNA quality and quantity was assessed with gel electrophoresis and a Broad Range RNA Qubit assay (Thermo Fisher Scientific, Waltham, MA). Illumina sequence libraries were prepared using the NEB Next Ultra Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resolved by electrophoresis at 150 V for 3 h at room temperature in Novex TBE-Urea Gels (EC6885BOX, Thermo Fisher Scientific) in 1x TBE buffer and cleaved and uncleaved products were imaged on ChemiDoc (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were subjected to SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels and MES running buffer (Thermo Fisher Scientific) according the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... All purified PCR products were visually checked for expected size via 1% agarose gel electrophoresis and assessed for purity and yield via Nanodrop (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was determined via agarose gel electrophoresis and the quantity was measured on a NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). An Illumina paired-end DNA library with an insert size of ∼300 bp was constructed using the Genomic DNA Sample Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was verified by agarose gel electrophoresis and used for TOPO cloning with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen). After selection on kanamycin ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 or 10 μg protein lysates were resolved in Novex 4-20% Tris-Glycine polyacrylamide electrophoresis gels (XP04205BOX; Invitrogen, Carlsbad, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2020Quote: ... The quality and quantity of extracted DNA were verified via gel electrophoresis (0.8% agarose) and photometrically using a NanoDrop ND 1000 spectral photometer (Thermo Fisher Scientific, USA). Extracted DNA was stored at −20°C until use ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (30-40 µg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (Thermo Fisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer dimers were removed by extracting PCR products using two lanes (10 μL each) of an E-Gel Power Snap Electrophoresis Device (ThermoFisher Scientific, Germany). This resulting library was sequenced on a MiSeq v2 250 bp PE Illumina at CeGaT (Tübingen ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the QIAquick Gel Extraction Kit after electrophoresis in agarose gel 1% and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
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bioRxiv - Genomics 2019Quote: ... DNA quality was assessed by gel electrophoresis (1% agarose in 1 × TAE containing ethidium bromide) and quantity was determined by Qubit Fluorometry (Invitrogen, California, USA) following manufacturer’s instructions.
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bioRxiv - Systems Biology 2019Quote: ... product quality was verified by DNA gel electrophoresis and sample DNA concentrations determined using Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The libraries were multiplexed together and sequenced using the paired-end with 250-bp paired-end reads approach on the MiSeq Illumina sequencing machine at the BioMicro Center (Massachusetts Institute of Technology ...
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bioRxiv - Genomics 2019Quote: ... The integrity and quantity of DNA was confirmed by 1.5% agarose gel electrophoresis and a nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, USA)
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bioRxiv - Synthetic Biology 2021Quote: ... Methylation was analyzed by DNA gel electrophoresis of 600 ng of DpnI- or MboI-digested plasmid DNA and GeneRuler 1 kb DNA Ladder (Thermo Fisher SM0313). All annotated plasmid sequences for the transactivator- and reporter-expressing plasmids are available in the Haynes Lab Benchling database (https://benchling.com/hayneslab/f_/mrI2t4mM-collection-dal-e/).
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bioRxiv - Bioengineering 2021Quote: Samples from time course experiments and the truncation screening were prepared for sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by adding 12 µL of NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) and 3 µL of tris(2-carboxyethyl)phosphine (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were run on 1% agarose gel electrophoresis for quality control and quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific Inc.). For each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 1 h at 37 °C the DNA products were analyzed by electrophoresis on a denaturing polyacrylamide gel (10%) and detected by chemiluminescent detection (Thermo Fisher Scientific). To semi-quantify the level of dCTP incorporation ...
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bioRxiv - Genomics 2022Quote: ... The quality and concentration of the extracted genomic DNA was assessed using 1% agarose gel electrophoresis and a Qubit fluorimeter (Invitrogen, Carlsbad, CA). This high-quality DNA was used for subsequent Nanopore and Illumina sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then separated in a 12% acrylamide gel using the Hoefer™ mini-Vertical Electrophoresis system (Thermo Fisher Scientific, USA) and the Laemmli-buffer system (40) ...
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bioRxiv - Neuroscience 2023Quote: ... 10 colonies of each npc1 heterozygous mutant were amplified and double checked by 1% agarose gel electrophoresis and Sanger sequencing with 3730xl DNA Analyzer (Thermo Fisher Scientific). Finally ...
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bioRxiv - Genomics 2023Quote: ... DNA concentration and quality were examined by electrophoresis on a 2% (w/v) agarose gel and a Qubit 3.0 fluorometer (Thermo Scientific, Wilmington, USA), respectively.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa and Salmonella organisms were prepared and separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) as described (18) with a Novex X-Cell Surelock minicell system (Invitrogen, Carlsbad, CA). Tris-bis-polyacrylamide gels (12.5% ...
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bioRxiv - Cancer Biology 2023Quote: ... Polyadenylation and integrity were assessed by gel electrophoresis in denaturing conditions and RNA was quantified with a Qbit fluorometer (Thermo Fisher Scientific). Purified RNA was resuspended in water at 1-10 μg·mL-1 and stored at -80°C until used.
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.