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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by 20 % denaturing polyacrylamide gel electrophoresis as above and stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Plant Biology 2021Quote: ... and the quality and quantity of the sample was assessed using agarose gel electrophoresis and a fluorometric procedure (Qubit Fluorometer, Invitrogen, USA) by the Beijing Genomics Institute (BGI ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were size-verified by electrophoresis on a 0.8% TAE agarose gel stained with SYBR Safe (Thermo Scientific, Waltham, MA) and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega ...
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bioRxiv - Immunology 2020Quote: ... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell ...
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bioRxiv - Microbiology 2020Quote: ... The quality and quantity of the DNA extraction were evaluated by 1% agarose gel electrophoresis and Qubit 3.10 Fluorometer (Invitrogen, Life, USA). The good-quality DNA was first sheared randomly to fragments of around 500bp by Focused-ultrasonicattor (Covaris M220 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were lysed in Laemmli buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (ThermoFisher, 88518). Membranes were blocked with 5% milk in TBST for 1h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al ...
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bioRxiv - Microbiology 2019Quote: ... After extraction the DNA was checked for quality by gel electrophoresis and quantified using a Qubit 4 (Thermo Fisher Scientific, U.S.A.).
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bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA concentration was evaluated through agarose gel electrophoresis and fluorometry using a Qubit 2.0 (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). Library preparation ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate reactions were pooled after PCR and amplified DNA was visualized by agarose gel electrophoresis and quantified by Pico green (Thermo Fisher). After cleaning with Ampure beads (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA concentration was measured through electrophoresis by loading 1μl of each sample on 1% agarose gel and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Synthetic Biology 2021Quote: ... All assembled plasmids (pFB66 to pFB72, pFB96 to pFB130) were checked correctly by agarose gel electrophoresis after FastDigest XbaI (Thermo Scientific) digestion ...
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bioRxiv - Plant Biology 2021Quote: ... DNA integrity was assessed using agarose gel electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a Bio-Tek Powerwave HT microplate reader ...
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bioRxiv - Microbiology 2021Quote: ... the quality of genomic DNA was evaluated by pulsed-field gel electrophoresis (Sage Science Pippin Pulse) and genomic DNA concentration was quantified using a Qubit 3.0 Fluorometer (Life Technologies Q33216). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl fractions were collected and analyzed by SDS–PAGE electrophoresis using 4–12% NuPage Bis-Tris precast gels (Life Technologies). Gels were stained with 0.01% (v/v ...
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bioRxiv - Genetics 2020Quote: ... PCR products were confirmed by agarose gel electrophoresis and sequentially either purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific) or gel-extracted using the QIAquick® Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was loaded and resolved by SDS-PAGE on a 12% Bis-Acrylamide gel and electrophoresis was carried out at using NuPAGE MES SDS running buffer (ThermoFisher Scientific). The separated proteins were transferred onto a nitrocellulose membrane (BioRad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of extracted protein was separated using Novex SDS– polyacrylamide gel electrophoresis reagents and transferred to nitrocellulose membranes (Life Technologies). Membranes were blocked in 5% milk protein and incubated with primary antibodies overnight ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of DNA was assessed by performing gel electrophoresis and the Qubit dsDNA BR Assay Kit (Thermo Scientific), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... were subjected to SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes or nitrocellulose membranes and blocked with I-Block (ThermoFisher, T2015). Primary antibodies used were ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration and quality were checked via gel-electrophoresis and UV-Vis spectrophotometry (NanoDrop 1000c, Thermo Fisher Scientific, Waltham, MA, USA). DNA was stored at −20 °C until further processing.
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bioRxiv - Molecular Biology 2023Quote: ... The remaining ∼10% of the cell lysate was then homogenized into a denaturing buffer solution containing 4X NuPAGE™ LDS Sample Buffer in preparation for polyacrylamide gel electrophoresis (Invitrogen™ NuPAGE™ ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of whole protein extracts were loaded and separated by electrophoresis on a 4–12% Bis-Tris gel (Life Technologies) and transferred to a PVDF membrane (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Fragmentation was verified using agarose gel electrophoresis and RNA quantity was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Synthetic Biology 2023Quote: ... Positive colonies were confirmed for correct assembly by enzymatic digestion (with BbsI for level 1 assemblies) followed by gel electrophoresis band size analysis (Invitrogen G401001).
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Immunology 2023Quote: ... Integrity and purity of purified proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie G-250 staining (ThermoFisher Scientific). Purified proteins were stored at –80°C until use.
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bioRxiv - Microbiology 2023Quote: ... Protein samples were subjected to a 4%-15% polyacrylamide SDS gel electrophoresis followed by electrotransfer to a nitrocellulose membrane (Fisher Scientific). The membrane was blocked with 3% milk ...
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bioRxiv - Immunology 2023Quote: ... then S-glutathionylated proteins were released and prepared for SDS polyacrylamide gel electrophoresis (SDS-PAGE) with reducing sample buffer at 100°C (ThermoFisher #39000); immunoblotting was performed with anti-PDCE2 antibodies (GeneTex #GTX109766) ...
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bioRxiv - Microbiology 2023Quote: PCR products were verified on a 2% agarose gel electrophoresis and the expected products were subsequently purified using ExoSAP-IT PCR Product Cleanup Reagent (Applied Biosystems) or Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... The lysates (20 - 30 μg of total protein) were subjected to electrophoresis on NuPAGE 4-12% Bis-Tris gels (Invitrogen, NP0322BOX), transferred to PVDF membranes (BIO-RAD ...
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bioRxiv - Cancer Biology 2023Quote: ... and the size of the amplicon was checked by electrophoresis of the PCR product on an agarose gel in TAE 1X (Life Technologies) (data not shown) ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The supernatants were quantified using the BCA kit (Pierce) and ∼10-30 µg total protein subjected to electrophoresis using NuPAGE 12% Bis-Tris gel in MES buffer (Thermo Fisher). The proteins were transferred to a 0.2-micron nitrocellulose membrane using a XCell II blot module (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... and purity were estimated using agarose gel electrophoresis (1%) and with a NanodropTM 2000 UV-Vis spectrophotometer (Thermo Scientific, Waltham, USA).
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bioRxiv - Systems Biology 2023Quote: ... The quality of total DNA was measured by 1% agarose gel electrophoresis and Nano drop 8000 Spectrophotometer (Thermo Scientific, Waltham, MA) (260 nm/280 nm ratio) ...
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bioRxiv - Genetics 2023Quote: ... The quality of the DNA was assessed visually by electrophoresis through 0.7% agarose gel and the amount quantified with a QubitTM following the manufacturer’s instructions (Thermo Fisher Scientific Inc.). DNA extractions were adjusted to a final concentration at 10ng DNA/μL.
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of total protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10-20% tris-glycine gradient (Invitrogen, XP10205BOX), transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (BioRad ...
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bioRxiv - Physiology 2023Quote: ... We performed electrophoresis with 20 μg/lane of each lysate in Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific). We transferred the protein samples onto polyvinylidene difluoride membranes with the iBlot2 Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Successful extractions were verified by visualizing DNA using gel electrophoresis as above and quantified using a NanoDrop 2000 (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to electrophoresis in 10% or 4–12% Bis-Tris gels and transferred to nitrocellulose membranes using iBlot2 (Life technologies). Membranes were probed for VEGF-C followed by horseradish peroxidase-conjugated secondary antibodies (Thermo Fischer Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 µg of protein per sample was separated by electrophoresis on 4-12% Bis-Tris Plus Gels (Life Technologies), transferred to nitrocellulose ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quality of DNA was checked using 1.5% agarose gel electrophoresis and concentration was measured by Qubit 4 fluorometer with 1X dsDNA HS Assay Kit (Thermo Fisher Scientific). As the basis for ddRAD library protocol development ...
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bioRxiv - Genetics 2023Quote: ... Samples were heated at 70 °C for 10 minutes prior to loading and electrophoresis on a 10% Bis-Tris polyacrylamide gel (ThermoFisher, #NP0316BOX), followed by electroblotting on to a PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: ... The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a NanoDrop2000 spectrophotometer (Thermo Scientific, United States). The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’)/ 806R (5’-GGACTACHVGGGTWTCTAAT-3’ ...