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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... We extracted the genomic DNA of ELISA-positive cattle using either the magLEAD 12gC or a MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific Inc., Waltham, USA) with an automated nucleic acid extraction system (KingFisher Duo Prime ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from fresh or frozen whole blood samples using either an ABI PRISM® 6100 Nucleic acid prep station (Applied Biosystems, Foster City, CA, USA) or QIAamp DNA Blood Mini Kits (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Denaturing electrophoresis and western blotting were performed using the NuPAGE® electrophoresis system (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... BMI1 level was assessed by western blot using standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
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bioRxiv - Cell Biology 2020Quote: ... H3K27me3 and H2AK119Ub modified histones were analyzed by standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
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bioRxiv - Cell Biology 2022Quote: ... samples (equivalent of 2 x 105 cells) were subjected to electrophoresis in NuPAGE Novex 4–12% Bis-Tris pre-cast gels (NP0321 from Life Technologies). The same number of cells (see FACS sorting of diploid and tetraploid cells section ...
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bioRxiv - Genomics 2020Quote: ... samples were heated for 5min at 70°C and size-separated by electrophoresis using 1.5mm 4-12% NuPAGE Bis-Tris protein gels and 1x NuPAGE MES SDS running buffer (Thermo Fisher Scientific) for 35-50min at 200V ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of the final reaction was subjected to electrophoresis on a 1.5% agarose gel using 2xRNA loading buffer (Thermo Fisher Scientific). A 1:1 mix of RNA and loading buffer was heated to 65°C for 5 min and then placed on ice to denature any secondary structure of RNA.
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... Native PAGE was conducted to characterize DNA nanostructures by using 4% TBE gel and 1x TBE buffer at a constant voltage of 200 V (electrophoresis system Thermo Scientific) in an ice water bath ...
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bioRxiv - Neuroscience 2019Quote: ... After the last wash PBS was completely aspirated and proteins were eluted from the beads and samples prepared for loading onto electrophoresis gels by adding 150uL-1X sample buffer (IP buffer 100ul buffer + 35uL of 4X NuPAGE™ LDS Sample Buffer ThermoFisher cat# NP0007 ...
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bioRxiv - Biochemistry 2019Quote: ... purity and integrity of the RNA were evaluated by agarose gel electrophoresis and on a ND-2000 spectrophotometer (Thermo Scientific, USA). For each RNA sample ...
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bioRxiv - Genomics 2020Quote: ... The integrity and concentration of RNA was analysed by agarose gel electrophoresis and using the Nanodrop ND-2000 spectrophotometer (Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... Samples with equal protein amounts were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using standard methods (89) and electroblotted onto polyvinylidene fluoride (PVDF) membrane (ThermoFisher, 88518). After blocking in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Immunology 2019Quote: ... 30 or 40 µg of total protein from tissue lystate was denatured and separated by SDS polyacrylamide gel electrophoresis and transferred to a PVDF membrane (IB401002, Life Technologies). Membranes were cut along the 55 kDa ladder marker and blocked with either 5% BSA or 5% dry skim milk in TBST for 1 hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... The protein samples were then size-separated by electrophoresis on a gradient Bolt 4-12% Bis-Tris plus gel (Invitrogen NW04120BOX), transferred on a polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA integrity was assessed using 1% (w/v) agarose gel electrophoresis and was quantified using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in 6X Laemmli sample buffer and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Thermo Scientific). Blots were probed with the indicated primary antibodies and with horseradish peroxidase (HRP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We assessed DNA quality by 0.8 % agarose gel electrophoresis and quantity by fluorometry using a Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). Samples were individually barcoded and pooled into a single library with the NEB Next Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the BRPF1 bromodomain was verified by SDS-polyacrylamide gel electrophoresis stained with GelCode Blue Safe protein stain (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified products were then resolved by electrophoresis using a 1.2% E-gel containing a SYBR Safe stain (Thermo Fisher Scientific), which was visualized on an E-Gel Imager Blue-Light Base (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... Protein concentration was determined by absorption spectroscopy and purity by SDS-PAGE electrophoresis on 4-12% NuPAGE gels in MES buffer (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from overnight bacterial cultures were boiled in LDS sample buffer and separated by electrophoresis using NuPAGE 12% polyacrylamide gels (Life Technologies). The contents of the gels were transferred to PVDF membranes ...
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Genomic and phenotypic characterization of finger millet indicates a complex diversification historybioRxiv - Genetics 2021Quote: ... The purity and quantity of extracted gDNA was determined by gel electrophoresis and a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA), respectively ...
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bioRxiv - Microbiology 2021Quote: The plasmid DNA yields from the sewage samples were evaluated using gel electrophoresis and Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA). Plasmid DNA purity was measured and validated by absorbance ratio of 260/280 and 260/230 using NanoDrop 100 (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The amplified products were resolved by gel electrophoresis in 2% Roti®agarose (Agarose High Resolution; Roth) in 0.5x UltraPureTM TRIS-Borat-EDTA (TBE) buffer (Thermo Fisher). Non-intron spanning primers for DAZL (DAZL(I)) ...
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bioRxiv - Microbiology 2021Quote: ... All in vitro transcribed RNAs were analysed by electrophoresis in 1.5% formaldehyde agarose gel and RNA concentration was determined on Nanodrop ND-1000 spectrophotometer (ThermoFisher Scientific, USA). RNA was transfected into cells using in-suspension protocol53.
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplicons were confirmed on a 1% agarose gel electrophoresis and purified with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Viral and cell lysates were resuspended in SDS sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... equal amounts of protein (typically 20-50 µg of protein per lane) were separated by electrophoresis on a NuPAGE 10% Bis-Tris Gel (Invitrogen, NP0315BOX) in NuPAGE™ MOPS SDS Running Buffer (20X ...
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bioRxiv - Cell Biology 2020Quote: ... Identity and purity of CIZ1-containing elution fractions was assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with SimplyBlue™ safe stain (Invitrogen) and pre-stained protein ladder 10-250 kDa (Thermo Scientific) ...
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bioRxiv - Physiology 2022Quote: ... Products were then analysed by 1% agarose gel electrophoresis in TBE buffer followed by SYBR Gold staining (catalog no. S11494, ThermoFisher Scientific). Gels were imaged with a ChemiDoc MP Imaging System ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of the retrieved DNA were assessed both before and after treatment via gel electrophoresis and Nanodrop analysis (ThermoFisher Scientific). Sequencing libraries for salivary samples were prepared using the NEBNext Ultra DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... The quality and quantity of DNA isolation across the 96 well plate were measured using agarose gel electrophoresis and Nanodrop (ThermoFisher Scientific), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were mixed with 6X sample buffer containing β-ME and were separated by SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels (Invitrogen), and transferred onto the 0.45 μm nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: ... and samples were heated to 95°C for 5 minutes and subjected to electrophoresis in 12% NuPAGE Bis-Tris gels (Life Technologies). Gels were transferred to nitrocellulose membranes (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: Viral and cell lysates were resuspended in sodium dodecyl sulfate (SDS) sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes and probed overnight at 4°C with the following antibodies in Odyssey Blocking Buffer (LI-COR) ...
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bioRxiv - Microbiology 2021Quote: ... and was then analyzed by electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA). DNA libraries were generated using a NEBNext UltraTM II DNA Library Prep Kit (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
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bioRxiv - Molecular Biology 2022Quote: ... were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo Scientific™) with a Hoefer TE77X semi-dry transfer unit at 20V for 1 h ...
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bioRxiv - Biophysics 2019Quote: ... Single strands were separated by electrophoresis in a 1.2% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific). After electrophoresis ...
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bioRxiv - Zoology 2019Quote: PCR products were run and purified by agarose gel electrophoresis and cloned into the pJET 1.2 cloning vector (Thermo Fisher Scientific). The inserts were sequenced.
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bioRxiv - Microbiology 2019Quote: ... Reaction products were separated by agarose gel electrophoresis and the amplicon derived from the extended array was purified from the gel using a GeneJET Extraction Kit (ThermoFisher Scientifc) and sequenced on a NextSeq 500 system.
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bioRxiv - Microbiology 2019Quote: ... Final cDNA libraries with ligated adaptors were size-selected to fall between 250 and 500 bp using the E-Gel® electrophoresis system (Invitrogen). cDNA quantity was measured using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... All samples were checked for quality and quantity with gel electrophoresis and fluorescence-based analysis (Qubit 2.0, Thermo Fisher Scientific, USA) before being used in a second PCR step with barcoded B341 forward primer and reverse B785 primer containing the P1 adapter ...
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bioRxiv - Developmental Biology 2019Quote: ... we eluted proteins and analyzed samples by electrophoresis on 4%–12% NuPAGE SDS-PAGE gels with MOPS SDS Running buffer (Life Technologies) and corresponding LDS buffer NuPage (Invitrogen ...