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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Bacterial DNA was stained using SYTO®41 Blue Fluorescent Nucleic Acid Stain (Molecular Probes #S11352, 1:200 vol/vol, wt/vol) in flow cytometry buffer (100 μL ...
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bioRxiv - Microbiology 2021Quote: ... samples were UV cross-linked and the biotin signal detected with HRP–conjugated streptavidin using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... total nucleic acids were resuspended in 50 μL ultrapure water and treated with DNAse I (Turbo DNA Free® kit; AM1907, Ambion®) to eliminate any trace of possible genomic DNA contamination ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of extracted nucleic acid was added to 0.8 μL of SuperScript® III RT/Platinum® Taq Mix (Invitrogen, Australia), 10 μL of 2x Reaction Mix ...
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bioRxiv - Microbiology 2020Quote: Flow cytometry analysis to measure parasitemia at the cycle of drug exposure was measured using the nucleic acid stain SYTO 11 (0.016 μM) (Life Technologies no. S7573) and a BD FACSCalibur machine as previously described (Rovira-Graells et al ...
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bioRxiv - Zoology 2022Quote: ... Aliquots of VTM supernatant were subjected to RNA/DNA extraction by MagMAX™ CORE Nucleic Acid Purification Kit (ThermoFisher Scientific, Waltham, MA). An aliquot of purified nucleic acid was tested by SARS-CoV-2 specific real time qRT-PCR to amplify the RdRp gene (20).
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bioRxiv - Neuroscience 2021Quote: Each dissected brain sample was digested with 8 µl of protease in 200 µl of digestion buffer from the RecoverAllTM Total Nucleic Acid Isolation Kit for FFPE (Thermo Fisher Scientific). Samples containing big chunks of brain tissue were homogenized in an electronic homogenizer ...
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bioRxiv - Cell Biology 2019Quote: ... RNA probes synthesized by T7-mediated transcription were purified by phenol-chloroform extraction and 1 mg RNA labelled with Alexa-546 using the ULYSIS Nucleic Acid Labeling Kit (Thermo Fisher (42). Nuclei were counterstained with Hoechst.
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bioRxiv - Immunology 2020Quote: ... Bacterial DNA was stained using SYTO®41 Blue Fluorescent Nucleic Acid Stain (Molecular Probes #S11352, 1:200 vol/vol, wt/vol) or with SYBR® Green I Nucleic Acid Gel Stain (Invitrogen #S-7563 ...
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bioRxiv - Molecular Biology 2021Quote: ... were plated for serological screening and high-throughput nucleic acid extraction using the MagMAX™ mirVANA and CORE pathogen kits (ThermoFisher, Australia).
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... The quantity and partial quality of nucleic acid samples were assessed based on absorbance spectrums using a spectrophotometer (Thermo Scientific, NanoDrop 1000).
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bioRxiv - Genomics 2019Quote: ... DNA was stained by adding 1 μl of 200x SYBR Green I Nucleic Acid stain (Invitrogen™, Thermo Fisher Scientific, MA, USA) and the sample was incubated for 20-50 min in the dark ...
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bioRxiv - Genomics 2019Quote: ... DNA was stained by adding 1 μl of 200x SYBR Green I Nucleic Acid stain (Invitrogen™, Thermo Fisher Scientific, MA, USA) and the sample was incubated for 20-50 min in the dark ...
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bioRxiv - Genomics 2021Quote: Viral RNA was extracted with MagMAX™ Viral/Pathogen Nucleic Acid Isolation kit in a KingFisher™ Flex Purification System (ThermoFisher Scientific). SARS-CoV-2 RT-qPCR was performed in a 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from nasopharyngeal and saliva samples using the MagMax Viral/Pathogen Nucleic Acid Isolation kit (ThermoFisher Scientific, Waltham, MA, USA), according to a modified protocol [3].
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bioRxiv - Cancer Biology 2021Quote: ... 50 nM of scrambled control or 5’-tRFCys antisense locked nucleic acid (LNA) oligos were transfected into cells with Lipofectamine 2000 (Thermo Fisher Scientific). Experiments were performed 24-48 hours post transfection ...
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bioRxiv - Microbiology 2020Quote: ... and the pellets were resuspended in 1 ml staining buffer supplemented with 0.5 μM Sytox Green Nucleic Acid Stain (Molecular Probes, Life Technologies), followed by incubation in the dark for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... and purity of nucleic acids in all faecal DNA was tested using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed by using the MagMAX CORE Nucleic Acid Purification KIT (Applied Biosystem, Termofisher Scientific, Life technologies corporation, TX, USA), following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA-protein complex was blotted to a positively charged nylon membrane and imaged using Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific). The primers used are listed in Supplemental Table S1.
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bioRxiv - Immunology 2023Quote: ... Extraction of SARS-CoV-2 RNA was performed using the MagMax Viral/Pathogen Nucleic Acid Isolation kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: We treated the fixed cells with Proteinase K before RNA extraction (RecoverAll Total Nucleic Acid Isolation Kit for FFPE, Thermo Fisher Scientific) to liberate RNA from protein-protein and protein-nucleic acid crosslinks generated by fixation ...
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bioRxiv - Physiology 2023Quote: ... Cells were loaded with 20 µM ThiolTracker Violet glutathione detection reagent and 100 nM SYTO 16 Green Fluorescent Nucleic Acid Stain (Invitrogen, catalog # S7578) and imaged within the hypoxic environment using an LS620 microscope (Etaluma ...
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bioRxiv - Biochemistry 2023Quote: ... were purified under denaturing conditions using the ultiMate 3000 HPLC system with an anion-exchange preparative DNAPac PA100 Nucleic Acid Column (both Thermo Fisher Scientific) as described before23 ...
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bioRxiv - Genomics 2023Quote: Automated magnetic bead-based nucleic acid extractions from 200 µl VTM specimen aliquots were performed in Kingfisher flex instrument (Thermo Scientific, USA). MagMAX Dx Prefilled Viral/Pathogen Nucleic Acid Isolation kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Nucleic acids concentration and absorbance ratio at 260/280 nm were assessed with the Nanodrop One spectrophotometer (Thermo Scientific, Wilmington, DE, USA) at an optical density of 260 nm (OD260).
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bioRxiv - Microbiology 2023Quote: ... and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit to extract from the pellets as specified in the manual (Applied Biosystems, Waltham, MA).
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bioRxiv - Immunology 2023Quote: ... After 18 h cells were mock transfected or transfected with 2-5A (20 μM) and stained for total cells with SytoTM 60 red fluorescent nucleic acid stain (250 nM) (ThermoFisher Scientific, #S11342) (converted to blue by IncuCyte software ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel, Germany) on a KingFisher Flex Purification System (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Gels were incubated at room temperature for 15 min with shaking in 50 ml 1X RNAse-free TAE mixed with 10 µl of 10000X SYBR Gold nucleic acid staining solution (Invitrogen™ S11494) and nucleic acids were visualized using a transilluminator ...
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bioRxiv - Immunology 2024Quote: DENV-2 and ZIKV viral RNA for real-time PCR assay was extracted from 200uL of virus isolate (previously tittered by focus reduction neutralization assay) and from acute serum samples using a MagMax Viral/Pathogen Nucleic Acid Isolation Kit (Applied Biosystems, MA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... for the discovery set and the Recover AllTM Total Nucleic Acid Isolation Kit for the French external validation cohort (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Pathology 2024Quote: Seahorse Mito Stress Tests were conducted as described.30 Data were normalized by total nucleic acid content measured by CyQUANT Cell Proliferation (C7026, Thermo Fisher Scientific) and analyzed by Wave 2.6.1 (Agilent) ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 1.43M β-mercaptoethanol was added to the samples to reach a 1X dilution (350 mM β-mercaptoethanol final concentration) before gel electrophoresis performed using 4–12% Bis-Tris gels (ThermoFisher Scientific, prod. number WG1402BOX). Proteins were transferred on Immobilon-FL membrane (Millipore ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized through electrophoresis on a 1·2% agarose gel stained with SYBR Safe DNA Gel Stain (Thermo Fisher Scientific, Waltham, MA, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... 25% of the supernatant fraction and 50% of the pellet fraction) were processed by denaturing polyacrylamide gel electrophoresis (SDS-PAGE) using Novex 4-20% gradient gels (Thermo Fisher Scientific, Waltham, MA, USA). For SDS-PAGE with standard reducing agents to disrupt sulfydryl bonds ...
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bioRxiv - Cancer Biology 2021Quote: Fifty μg of protein lysate per lane was resolved on NuPAGE gels using the Novex electrophoresis system (Invitrogen) at 200 V ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were screened through 1% agarose gel electrophoresis and purified using ExoSAP PCR Product Cleanup Reagent (Thermofisher). Amplicons were Sanger sequenced by Macrogen Europe Lab ...
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bioRxiv - Genomics 2020Quote: ... PCR products were analysed by agarose gel electrophoresis with a GeneRuler 100 bp Plus DNA ladder (ThermoFisher SM0321). If additional bands ...
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bioRxiv - Cancer Biology 2019Quote: After verification of the PCR reaction success by agarose gel electrophoresis using SYBR safe stain (Thermo Fisher Scientific) on a single 50 μL reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Intermolecular G4 formation was confirmed by native gel electrophoresis followed by staining with SYBR® Gold (Life Technologies), as described (34) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA quality was assessed by agarose gel electrophoresis and quantified using the Qubit dsDNA BR Assay Kit (Thermofisher). Whole genome libraries for use in bait capture were prepared using Ion Plus Fragment Library kits according to the manufacturer’s instructions with a target insert size of 400bp and unique Ion Xpress barcodes for each sample ...
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bioRxiv - Developmental Biology 2021Quote: Whole-cell lysates and immunoprecipitated proteins were subjected to NuPAGE 4%-12% bistris acrylamide gel electrophoresis (Life Technologies). Using standard protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA integrity was verified using agarose gel (1%) electrophoresis stained with 0.5x SYBR® Green (Invitrogen®) and observed under UV light ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified products were separated by electrophoresis in an agarose gel (1%) stained with 0.5x SYBR Green (Invitrogen).
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bioRxiv - Genomics 2020Quote: ... and separated by electrophoresis in MOPS buffer at 200 V in a Bolt gel tank (Thermo Scientific #A25977) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were subjected to electrophoresis on NuPAGE 4% to 12% Bis-Tris gels (NP0321; Thermo Fisher Scientific). Proteins were transferred to polyvinylidene fluoride membranes and probed using anti-p62 (SQSTM1 ...