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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with 200 nM of a solution of SYBR Green I (SGI) fluorescent nucleic acid stain (Molecular Probes, Life Technologies Corp.) for 45-60 min in the dark at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Both genomic DNA and cDNAs were analyzed on DT96 real-time DNA amplifier (DNA-Technology) using SYTO™ 13 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific). RT-qPCR values were normalized to the rp49 mRNA and genomic DNA qPCR values were normalized to rp49 genomic qPCR ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Biochemistry 2019Quote: ... The washed cells were then resuspended in 1mL of PBS and stained with SYTO 17 red fluorescent nucleic acid stain (Molecular Probes, Inc. OR) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific, Eugene, OR, USA) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Zoology 2020Quote: ... Viral RNA was extracted using a MagMAX CORE Nucleic Acid Purification Kit on a 96-well Kingfisher Flex System (ThermoFisher Scientific, Waltham, MA). A subset of samples was tested for RNA concentration on an Epoch Microplate Spectrophotometer (BioTek ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... the supernatant was obtained and the nucleic acids in there were measured by absorbance at 260 nm using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2020Quote: ... ASO knockdowns were performed with 50 nM (final concentration) locked nucleic acid (LNA) GapmeRs transfected with Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA). All ASOs were modified with phosphorothioate (PS ...
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bioRxiv - Biochemistry 2021Quote: ... Cell culture supernatant was harvest 42 h post-infection and viral RNA was purified using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific). The samples were processed using the semi- automated NucliSENS® easyMag® platform (bioMérieux ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then cross-linked at 120J/m2 and then labelled RNA was detected using the Chemiluminescent Nucleic Acid Detection module kit (Thermofisher, cat n°: 89880) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: RNA extraction from cell lysates was performed using the MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit (Catalog number: A48383) (Applied Biosystems®) and carried out according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... nucleic acid quantification was measured using either Qubit™ RNA HS Assay Kit or Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific), as appropriate.
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration and purity of the extracted nucleic acids were checked using a NanoDrop 2000c UV-Vis spectrophotometer (Thermo Scientific, Waltham, MA, USA). DNA extracts were stored at -25 °C until use ...
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bioRxiv - Cell Biology 2023Quote: The RNA from in situ fixed and sorted cells (each replicate derived from a minimum of 3 mice of the same sex and age) was isolated using the RecoverAll™ Total Nucleic Acid Isolation Kit for formalin-fixed parafilm embedded (FFPE) samples (Invitrogen, cat#AM1975). Library preparation ...
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bioRxiv - Bioengineering 2023Quote: Cells grown to mid-log phase were fluorescently labeled using 20 μM SYTO® BC Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific) in their growth medium ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were blocked in 200 μL buffer W (GeoMx RNA Slide Prep FFPE-PCLN kit) for 30 minutes and incubated at 4°C overnight with SYTO 13 nucleic acid stain (500 nM; Thermo Fisher Scientific #S7575) and antibodies for pan-cytokeratin (panCK ...
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bioRxiv - Molecular Biology 2024Quote: ... coli strain) on the automated KingFisher Flex platform using the MagMax™ Viral/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific, UK). Post extraction DNase treatment was performed using the TURBO DNA-free™ Kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acids were extracted using the MagicPure® Stool and Soil Genomic DNA Kit and Kingfisher Flex Purification System (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... for the discovery set and the Recover AllTM Total Nucleic Acid Isolation Kit for the French external validation cohort (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... Gels were run using 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Invitrogen NP000202) and transferred onto nitrocellulose transfer membrane using an XCell II Blot Module or iBlot Dry Blotting System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... gels were rehydrated in 3% acetic acid and successively stained using Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (Invitrogen) and Coomassie Brilliant Blue R-250 ...
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bioRxiv - Immunology 2019Quote: ... Bacteria were labeled with 5 μM Syto™ 9 Green Fluorescent Nucleic Acid Stain or 500 nM pHrodo Red SE (ThermoFisher Scientific, Waltham, MA) and washed 3-5 times with PBS prior to infection ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with 200 nM of a solution of SYBR Green I (SGI) fluorescent nucleic acid stain (Molecular Probes, Life Technologies Corp.) for 45-60 min in the dark at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 100 μL of a broth culture was extracted on the Applied Biosystems MagMAX 96 automated nucleic acid extraction platform (Applied Biosystems, Foster City, CA) and eluted in a final volume of 90 μL ...
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bioRxiv - Microbiology 2022Quote: WGS performed at CFIA used extracted nucleic acid quantified using the Qubit™ RNA High Sensitivity (HS) Assay Kit on a Qubit™ Flex Fluorometer (Thermo Fisher Scientific). 11uL or 200ng of total RNA was subject to DNase treatment using the ezDNase™ Enzyme (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was isolated from the samples using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit and KingFisher ™ Flex Purification System (ThermoFisher Scientific, US). The TaqPathTM COVID-19 CE-IVD RT-PCR kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... the probe mixture was heated to 95°C for 5 min to denature it and then incubated for 30 min at 80°C with 10 µL of the red emission dye Alexa594 (Ulysis™ Alexa Fluor® 594 Nucleic Acid Labeling Kit, Thermofisher, cat.no: U21654). The unbound Alexa594 was removed using chromatography columns (Micro Bio-spin chromatography columns P-30 ...
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bioRxiv - Microbiology 2019Quote: ... day 1 (24 hours) and day 3 (72 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific, Eugene, OR, USA) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Genomics 2021Quote: ... High molecular weight DNA was extracted from muscle and fin clip using MagMAX CORE nucleic acid purification kit (Thermo Fisher Scientific, Carlsbad, CA, USA) to produce PacBio continuous longs reads (CLR ...
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bioRxiv - Genetics 2021Quote: ... The purity and concentration of the purified nucleic acid were determined by measuring the absorbance at 260 nm and 280 nm using a NanoDrop 2000 spectrophotometer (Thermo Fisher, Waltham, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... The concentration of nucleic acid in the libraries was measured using a Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and 1.13 ng of each sample were pooled in 80 μl of nuclease-free water ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred onto a nitrocellulose membrane (Cytiva, cat#10600001) for Western blotting or for RNA detection using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific, cat#89880).
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bioRxiv - Microbiology 2023Quote: ... 500 μL of the microbial cell suspension was stained with the nucleic acid dye SYTO™ 9 (Thermo Fisher Scientific, 0.5 μM in DMSO) for 15 min in the dark ...
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bioRxiv - Synthetic Biology 2023Quote: ... The gels were then rinsed three times in water for an hour each and later stained with SYBR gold nucleic acid stain (ThermoFisher Scientific)(DNA Stains | Thermo Fisher Scientific - ES, n.d.) for 1 h before visualization under a UV light ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 L of untreated sewage was collected and processed (150 mL) using ammonium sulphate precipitation and nucleic acid extraction using a Kingfisher Flex™ (Thermo Scientific™, UK) and NucliSENS® (BioMérieux ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted for nanopore sequencing using the automated KingFisher Flex platform using the MagMax™ Viral/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific, UK). DNA was sequenced on an ONT GridION using the rapid barcoding kit (SQK-RBK004 ...
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bioRxiv - Microbiology 2019Quote: ... Blue native electrophoresis of the concentrated complexes was carried out on a 3-12% Bis-Tris gel (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The libraries were selected from 250–350-bp fragments obtained by E-Gel 2% SizeSelect electrophoresis (Invitrogen, cat# G661012). Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then subjected to SDS gel electrophoresis before being transferred to nitrocellulose membranes using iBlot2 (Life Technologies), blocked with 5% BSA in TBS with 0.1% Tween-20 and incubated with primary antibodies ...
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bioRxiv - Genomics 2020Quote: ... The integrity and purity of the RNA were determined by gel electrophoresis and a NanoDrop ND-1000 (Thermo Scientific), respectively ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of 16S rRNA gene products was performed by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Amplicons were visualized on 0.8% agarose gel electrophoresis using 1× TAE buffer stained with SYBRSafe (Invitrogen, Carlsbad CA, USA), and compared to a GeneRuler 1 Kb Ladder (ThermoScientific ...
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bioRxiv - Genetics 2020Quote: ... Quality/quantity of the extracted DNA was evaluated by gel electrophoresis and Qubit dsDNA BR Assay Kit (Life Technologies). To evaluate the probability of DNA degradation ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of merA and merAB gene products was performed by Genoscreen (Lille ...
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bioRxiv - Neuroscience 2021Quote: ... extracted nuclear proteins were subjected to NuPAGE Bis-Tris (4 to 12%) gel electrophoresis under reducing conditions (ThermoFisher Scientific) and then electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...