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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stored in IC fixation buffer at 4°C until analysis on the Attune NxT Flow Cytometer (ThermoFisher Scientific) when they were centrifuged for 5 min at 400g and dissolved in FACS buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 µl of cell lysates were separated on 4-12% tris-glycine SDS-polyacrylamide gel electrophoresis (SDS-PAGE) from Invitrogen, and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 10 min at 90°C and loaded on a 4–20% Tris-Glycine precast gel (ThermoFisher). Protein was transferred onto a nitrocellulose membrane using the iBlot Dry Blotting System ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty μg of total protein extracts and 10 μg of nuclear extractions were loaded on 4-12% Bis-Tris gels (Invitrogen) in SDS-MES running buffer ...
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bioRxiv - Cell Biology 2020Quote: ... human biopsies or hind limb muscles for each mouse were minced and put into a 15 mL tube containing 4 mL of HBSS (#24020-091, GIBCO) BSA (0.2% ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from groups of 5 HIOs per replicate with a total of 4 replicates per infection condition using the mirVana miRNA Isolation Kit (ThermoFisher). Cytosolic and mitochondrial ribosomal RNAs were removed from samples using the Ribo-Zero Gold Kit according to manufacturer’s protocol (Illumina) ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Biochemistry 2021Quote: ... The flow through was collected and analysed by SDS-PAGE (NuPAGE Bis-Tris 4-12% Pre-cast gels, ThermoFisher Scientific), concentrated using 10,000 molecular weight cut off (MWCO ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then run on 4%–12% Bis Tris gels and transferred onto nitrocellulose membranes using an iBlot (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Bioengineering 2021Quote: ... a matrix of 20 well (row) × 29 well (column) in nine NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) was coated overnight at 4°C with 4 µg/mL streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 75 μg of protein was loaded and samples were run on a NuPAGE 4-12% Bis-Tris Gel in MOPS SDS Running Buffer (Invitrogen). After separation by electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... 10-40 μg of proteins were loaded on a precast gel NuPAGE Novex 4-12% Bis-Tris (Thermo Scientific, NP0336BOX). Proteins were then transferred onto a nitrocellulose membrane using an iBlot2 dry blotting system with iBlot2 transfer stacks (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 100 µg of mitochondria were pelleted at 10,000 x g for 10 minutes at 4°C and resuspended in 1x pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
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bioRxiv - Neuroscience 2020Quote: ... tissue was placed on slides and cover-slipped with mounting media containing DAPI (Fluoromount-G; 4’,6-diamidino-2-phenylindole; Invitrogen). All immunofluorescence runs contained tissue from more than one age (1 ...
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bioRxiv - Cancer Biology 2021Quote: ... with the exception of OCT3/4 and Nanog which were taken from Chen et al 201735) were diluted in PCR grade nuclease-free water (Gibco) at a concentration of 100 µM ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated for 30 minutes at 4°C with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher Scientific) while in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The health of the cell culture was assessed in a test sample by treatment with 50µM Bic treatment and 75µM 4-Aminopyridine (4AP; Acros Organics, #104571000).
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bioRxiv - Biophysics 2021Quote: The purified proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% Bis-Tris NuPAGE gels (Invitrogen). Prior to SDS-PAGE analysis ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Neuroscience 2022Quote: Freshly prepared solubilized proteins were incubated o/n at 4°C with affinity-purified rabbit anti-P2X4 antibodies (Alomone) cross-linked to magnetic beads (Dynabeads, Invitrogen). The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were plated in 10cm dishes at 80-90% confluency the day before transfection and transfected 16 hours later with 4 μg total DNA (2 μg of each plasmid) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... An equal volume of each PCR was then pooled and 100 µL were used for gel extraction from a 4% E-Gel EX Agarose Gel (Invitrogen). The fragment size and quality of the extracted DNA were tested using a 2100 Bioanalyzer system (Agilent) ...
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bioRxiv - Developmental Biology 2022Quote: ... ex vivo: 20 μg) of protein lysates as described above were resolved on 4- 12% Bis-Tris Protein gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The cells were placed onto 4 ml MSgg agar (1.5% w/v) set into each chamber of LabTek II 2-chamber microscope vessels (Thermo Scientific) set into various containers ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and cells were switched into maintenance medium: William’s E medium supplemented with 4 % hepatocyte maintenance supplement (Thermo Fisher Scientific; AI15564), 2 % fetal bovine serum ...
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bioRxiv - Genetics 2022Quote: ... Lysates were centrifuged at 15,000g for 15 min at 4 °C and quantified by the Bradford Protein Assay (Thermo Scientific). SDS-PAGE gel was used for protein electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: HTM hydrogels on coverslips subjected to the different treatments for 10 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at 4ºC overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA concentrations were measured using the Qubit™ RNA High Sensitivity Kit and a Qubit 4 fluorometer (ThermoFisher, Schwerte, Germany). Equal quantities of the samples were used to create independent replicate pools for each stage ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Cy5-pDNA/PP-sNp or naked Cy5-pDNA for 4 h in Opti-MEM I Reduced Serum Medium (31985062, Invitrogen). The cells were further collected for the analysis of mean fluorescence intensity (MFI ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 pmol of siRNA was mixed with 4 µl of Lipofectamine™ RNAiMAX Transfection Reagent (Cat. No. 13778030, Thermofisher scientific) in Opti-MEM™ I Reduced Serum Medium and incubated for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with PBS and pelleted by centrifugation (450 x g, 10 min, 4 °C) prior to being stored at -80 °C in TRIzol Reagent (Invitrogen). RNA was extracted with the Direct-zol RNA Microprep kit as per manufacturer’s instructions (Zymo Research R2062) ...
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bioRxiv - Genetics 2022Quote: ... 50 µL of media was removed and replaced in each well by a 2x calcium dye solution consisting in Fluo-4 NW dye (Invitrogen), 1.25 mM Probenecid F-127 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... using streptavidin-coated microplates and a monoclonal antibody to detect the CD4d3+4 tag (50) (Nunc Immobilizer, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... using streptavidin-coated microplates and a monoclonal antibody to detect the CD4d3+4 tag (50) (Nunc Immobilizer, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and 120 ug of protein were resolved on a gradient SDS-PAGE A total of 120 μg of protein from mixed lysates was migrated on a pre-cast 4-12% Novex NuPAGE Bis-Tris SDS Mini Gel (Invitrogen). After Coomassie staining ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 20,800 g at 4°C for 10 min and supernatant protein concentrations quantified using the Bradford assay kit (ThermoFisher, #23236)
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bioRxiv - Microbiology 2022Quote: ... An equal amount (~25 μg) of the proteins were loaded on a NuPAGE 4-12% Bris–Tris gradient gels (Invitrogen) as previously described82 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein purity was determined by resolving purified protein by SDS–PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) at 200 V ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant obtained after the centrifugation of the lysate at 9000 rpm for 25 minutes at 4°C was subjected to overnight binding with Hi-bind Ni-NTA agarose beads (Invitrogen). The beads were washed 4-5 times with wash buffer (20mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were supplemented with 4 μL of 6x loading buffer (30% glycerol, bromphenol blue, xylene cyanol) and were resolved by polyacrylamide gel electrophoresis in 4-12% TBE Gels (Invitrogen) in 1%TBE buffer at 100 V for 45 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Equal protein amounts were run on 4-12% Bis/Tris gels in 1X NuPAGE MOPS SDS Running Buffer (ThermoFisher Scientific). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2019Quote: ... FDB fibers are stained at room temperature for 20 min prior to imaging with 5 µM of the calcium-sensitive dye mag-fluo-4 AM (KD ~22 µM, Invitrogen) dissolved in Tyrode’s buffer.
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bioRxiv - Synthetic Biology 2019Quote: ... benthamiana leaves infiltrated with SN14 (MTP-Su9::NifD::HA) were run on SDS-PAGE using a gel having a polyacrylamide concentration of 4-20 % (Invitrogen). The gel was stained with Aqua stain (Bulldog Bio) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cytocentrifuged preparations (200 μL of cell suspension/slide) were obtained in a Cytospin 4 Shandon (Thermo Scientific Corporation, Waltham, MA) at 800 rpm for 5 minutes at room temperature and stained with LIVE/DEADTM viability/cytotoxicity kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Molecular Biology 2019Quote: ... Ten micrograms of cell lysate or 20 µg of exosomal protein was separated on gradient SDS-polyacrylamide gel (4-12% Bis-Tris, Invitrogen) and transferred to nitrocellulose membrane (Trans-Blot Turbo ...