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Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells (ATCC) were transfected with 4 µg ID91-repRNA or GFP-repRNA as a negative control using lipofectamine 2000 (ThermoFisher). After 24 hours cells and supernatants were collected for analysis by SDS-PAGE and Western blot under reducing and non-reducing conditions ...
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bioRxiv - Immunology 2022Quote: ... Sections were then washed 3 x 30 min in TBST and incubated overnight at 4°C with appropriate secondary antibodies raised in goat and conjugated to Alexa Fluor dyes (Invitrogen) were diluted 1:500 or 1:1000 in TBS-T + 2 % NDS/NGS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Pathology 2022Quote: ... Samples were centrifuged (1,600g, 10min., 4°C) and supernatant absorbance was read (λ540 nm) in a Multiskan Sky microplate reader (Thermo Scientific). Serial dilutions of purified MDA were used as a standard and MDA concentration was expressed as µmol MDA/g wet weight tissue.
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 405 WT peptides were identified (Table 4) on the Fusion Lumos OrbiTrap mass spectrometer (mass tolerance <5 ppm) using Proteome Discoverer 2.2.0 software (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... they were separated by 1D SDS/PAGE using Invitrogen NuPAGE 4-12% Bis-Tris midi gels (ThermoFisher Scientific, Massachusetts, USA) and Invitrogen MES running buffer using the manufacturer’s instructions (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were denatured at 75 °C for 20 min and run at 150 V for 1.5 h on 4-12% NuPAGE Bis-Tris gels in NuPAGE MOPS running buffer (Thermo Fisher). Proteins were transferred to polyvinylidene difluoride membrane using NuPAGE transfer buffer (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were boiled at 95°C for 5 min and loaded on SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). Efficiency of ligand coupling to the Ad capsid (% total hexon protein coupled ...
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bioRxiv - Genomics 2022Quote: ... and equal total protein amounts were loaded and run on a precast NuPAGE Bis-Tris 4–12% (Thermo Fisher Scientific) SDS–PAGE at 120 V ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The cryo-EM grids were prepared at 100% humidity and 4 °C using a Vitrobot Mark IV (Thermo Fisher Scientific) and the following blotting parameters ...
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Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... followed by centrifugation at 5000g for 20 minutes at 4°C and RNA was isolated using TRIzol reagent (Life Technologies), as described previously by Abhishek et al.10 ...
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bioRxiv - Microbiology 2022Quote: ... pseudovirus carrying a luciferase reporter and encapsulated in Omicron variant S proteins were incubated with six 4-fold serial dilutions of the heat-inactivated serum samples by DMEM (Gibco) for 1 h at 37℃ ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated to 90°C for 10 min before being loaded on 4-12% Bis-Tris polyacrylamide gels (ThermoFisher). Each experiment was triplicated ...
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bioRxiv - Genetics 2022Quote: ... fixed ovaries and eggs were incubated in a 300 nM DAPI stain solution (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) on a shaking platform (150-200 rpm ...
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bioRxiv - Bioengineering 2022Quote: ... Netherlands) and shock frozen in liquid nitrogen to generate cryosections of 4 μm on a Cryostar NX70 (Thermo Fisher Scientific). Sections were mounted on Superfrost Plus slides (Menzel Glas ...
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bioRxiv - Cell Biology 2022Quote: Human and mouse lung samples were inflated with a 4% w/v paraformaldehyde (PFA) solution in PBS (14190250, Thermo Fisher) then placed in 4% PFA overnight for fixation ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were passaged every 4-5 days using 0.5 mM EDTA (pH 8.0) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) for 10 minutes and kept in phosphate-buffered saline (PBS, no. 10010023, Gibco) during the experiment.
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bioRxiv - Biophysics 2022Quote: ... operated at 200 kV and equipped with a Selectris energy filter and a Falcon 4 detector (both Thermo Fisher Scientific). Images were recorded in Electron Event Representation (EER ...
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bioRxiv - Biochemistry 2022Quote: ... a fraction of these (amount corresponding to approximately 0.5 pmoles of total ID2) were loaded on 4-12% Wedge-Well Tris- Glycine gels (Thermo Fisher). Following SDS-PAGE ...
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bioRxiv - Bioengineering 2022Quote: ... CK+DCIR medium consists of epithelial base medium (50% DMEM [Gibco], 50% F-12 Nutrient Mixture [Gibco], 15 mM HEPES [Corning], 4 mM L-glutamine [Gibco)] 1% P/S [Gibco] ...
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bioRxiv - Bioengineering 2022Quote: ... The dissociated cells and tissue fragments were centrifuged at 1,000 rpm for 5 mins at 4 ℃ and cultured in a T75 tissue-culture flask in a growth medium (advanced DMEM/F12 (Gibco) containing 5% bovine serum (Gibco)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Physiology 2022Quote: ... Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen). Proteins were transferred onto polyvinylidene fluoride membranes (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of detergent-soluble lysates were resolved using the NuPAGE Novex Midi Gel system on 4–12% Bis–Tris gels (Invitrogen). Proteins were transferred to PVDF membranes (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: ... The mitochondrial suspension was then centrifuged at 8500xg for 20 minutes at 4°C and the remaining pellet was resuspended in TRIzol reagent (15596018, Invitrogen) for downstream extraction of mitochondrial RNA using Direct-zol RNA miniprep (R2055 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed using the Avestin Emulsiflex c5 (ATA Scientific) and centrifuged at 4°C for 15 minutes at 12’000 RPM in the SS-34 rotor (Thermo Scientific). Supernatant was filtered through a 0.45 μm filter ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 14,000 rpm for 20 min at 4°C and cleared with protein A-Sepharose beads (Thermo Scientific). Pre-cleared lysates were then incubated with anti-GluN2A-pS1459 coupled to protein A-Sepharose overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: Phosphopeptide enrichment was performed using a Fe3+-IMAC column (Propac IMAC-10 4×50 mm, Thermo Fisher Scientific, Waltham, USA) as described previously (Ruprecht et al. ...
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bioRxiv - Immunology 2020Quote: ... cells were restimulated with PMA (50 ng/ml) and ionomycin (500 ng/ml) for 4 h in RPMI 1640 (Invitrogen) containing 8% FCS ...
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bioRxiv - Immunology 2020Quote: ... CAR T cells were incubated for 30 min at 37°C with either 1μM fura-2 AM or fluo-4 AM (Life Technologies). T cells were then washed in HBSS and resuspended in TexMACS complete medium with 3% AB serum ...
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bioRxiv - Microbiology 2020Quote: ... a single colony was selected to in-oculate 4 ml Luria-Bertani (LB) broth (Thermo Fisher Scientific, Waltham, MA, USA) containing vector-specific selective antibiotics (25 μg/ml for Kanamycin or 50 μg/ml for Ampicillin) ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted cell lysates were incubated for 16 h at 4°C with Dynabeads Protein G (100-O3D, Thermo Fisher Scientific) precoated with 5 μL of anti-MYC antibody (ab32 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated at 95 °C for 10 min before loading onto a 4-12% gradient Bis-Tris mini-gel (Invitrogen). After separation ...
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bioRxiv - Neuroscience 2019Quote: Mice were terminally anaesthetised with intraperitoneal injection of pentobarbitone followed by thoracotomy and transcardial perfusion with 10 ml 0.9% NaCl followed by 20 ml 4% paraformaldehyde (PFA, Fisher Scientific) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then incubated overnight with primary antibody at 4°C followed by an incubation with Alexa Fluor-conjugated anti-donkey-secondary antibodies (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Separation of an equal amount of proteins by SDS-PAGE was performed using 4-12% NuPAGE Tris-Acetate gels (Invitrogen). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... cells reached 90-95% confluency and were transfected with siRNA twice (4 hours apart between first and second transfections) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilised protein was cleared by centrifugation (10 min, 125000xg, 4°C) and incubated with antibodies cross-linked to Dynabeads (Invitrogen) by overhead rotation for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mg of pre-cleared HeLa nuclear extracts were incubated overnight at 4°C with either 40 μg ATRX antibody or rabbit IgG cross-linked to Dynabeads Protein G (Invitrogen). Beads were washed three times with 5 ml wash buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved by gel electrophoresis using the NuPage Bis-Tris 4-12% precast gel system according to the manufacturer’s instructions (Life Technologies). Western blot transfer was carried out according to the manufacturer’s instructions with the SureLock X-cell system (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4 °C in TBST-5% BSA—a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on acrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: Protein lysates were obtained from all cell lines using RIPA buffer and separated in a NuPAGE Bis-Tris 4-12% gradient gel (ThermoFisher). Proteins were transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... RELN or different RELN mutants (Y1821H, G1280E, I650S, D556V, R913C, C539R, R3207C) was performed for 4 hrs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 18 μg of total protein were resolved under reducing conditions by using SDS polyacrylamide gels and NuPage Novex 4–12% Bis/Tris Gels (Invitrogen) for SUMO conjugates analysis or 10% for SAE2 immunodetection ...
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bioRxiv - Neuroscience 2021Quote: ... a total of 5 ug of reduced protein lysates were loaded onto a 4-12% gradient Bis-Tris gel in 1X SDS Running buffer (Invitrogen) supplemented with the NuPAGE antioxidant ...