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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... the islets or cells were treated with Fluo-4 direct calcium reagent solution containing 2.5 mM probenecid (Thermo Fisher Scientific) for 60 min at 37°C and washed and then stimulated with 75 mL KCl for 1 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were incubated for 20 min at 4 °C with gentle agitation and lysed by sonication (Model 500, Fisher Scientific). Lysate was repeatedly clarified by centrifugation (35,000xg for 30 min) ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 μl of boiled supernatant of whole cell lysates was resolved on 4-12% Bis-Tris NuPAGE protein gel (Invitrogen) in 1×MES buffer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... in non-reducing conditions and the proteins were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies). Proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly,10μg of denatured protein was loaded onto Novex 4-20% Tris-Glycine Mini Gels (Thermo Fisher Scientific, MA, USA) and subjected to electrophoresis (45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Final RNA concentration and quality were measured with a Qubit 4 Fluorometer using the HS RNA kit (Thermo Fisher Scientific), and a Nanodrop OneC ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of total protein were loaded per well on Bolt™ 4–12 % Bis-Tris gels (Thermo Scientific, #NW04120BOX) and blotted on a 0.2 µm PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Proteins were mixed with NuPAGE reducing sample buffer and boiled at 70°C for 10 min and separated by SDS-PAGE on NuPAGE 4–12% Bis-Tris gels in anti-oxidant buffer (all from ThermoFisher). Resolved proteins were transferred to a nitrocellulose membrane using the Trans-blot Turbo system (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 100μg of whole cell lysate from each strain was run on NuPage 4-12% bis-tris protein gel (Thermo Scientific) at 120V for 3hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Every time point was fixed with paraformaldehyde 4% overnight at 40C and then dehydrated and rehydrated through methanol and PBS 1x (Gibco)-Tween 5% washes ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane and the membranes were probed overnight at 4 °C with the appropriate primary antibodies recognizing SLITRK6 (Invitrogen) or β-Actin (ACTB ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 4 μg RNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA). Real-time PCR was performed on the ABI 7500 FAST qPCR system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blotting was completed using 4-12% Bis-Tris pre-cast gels and MOPS running buffer purchased from Invitrogen (USA), Nitrocellulose membrane obtained from GE Healthcare (Amersham ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples (50µg total protein) were resolved by SDS-PAGE using 4-12% Bis-Tris gel (Nupage, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... protein samples were separated using pre-cast 4%-12% Bis-Tris Gels (Thermo-Fisher) and stained using the Silver Quest Staining Kit (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: Harvested cells were centrifuged on glass slides by a cell cytospin machine (Cytospin 4, Thermo Fisher Scientific, Waltham, MA, USA). Morphology and membrane proteins were observed by MGG and IF staining respectively as described previously40 ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoblots were analysed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 2 min at 90 °C and 18 μL of resuspended cross-linked pellets were loaded onto a 4-12% pre-cast Novex Tris-Glycine gels (Invitrogen). Further immunodetection of caspase-4 was performed following standard western blotting procedures.
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bioRxiv - Cell Biology 2020Quote: ... all the crosslinking samples were boiled for 10 min and resolved on a NuPAGE 4–12% gradient polyacrylamide gel (Invitrogen). Gels were stained using SimplyBlue (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled LDMNCs were incubated with Biotin Binder Dynabeads at a ratio of 4 beads per input cell (Life Technologies) and the lineage-positive cells were depleted using a magnetic particle concentrator according to the manufacturer’s instructions (Dynal MPC-6 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were resolved by SDS-PAGE using Novex NuPAGE pre-cast gradient Bis-Tris acrylamide gels (4-12%) (Invitrogen). After gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... Cells were incubated with 4 ng/µl of custom labelled goat anti-rabbit IgG – PeG4-ATTO655 (DOL 1.7) secondary antibodies (Invitrogen, 31212) or custom labelled goat anti-mouse IgG – Alexa647 (DOL 1.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and equal amounts of protein (20-40 μg) were run on NuPage 4-12% Bis-Tris gradient gels (Thermo Fisher) by SDS-PAGE and transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA content analysis was performed by fixing cells with 4 % PFA and staining with FxCycle Violet Stain (Thermo Fisher Scientific) followed by FACS using a MACSQuant Flow Cytometer (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: Denatured protein lysates (15 μg/sample) were loaded on to NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gels (Invitrogen, NP0321) alongside Chameleon Duo Protein Ladder (3 μL/lane ...
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bioRxiv - Cell Biology 2020Quote: ... 20-25 μg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P ...
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bioRxiv - Cell Biology 2020Quote: ... cells were blocked at room temperature for 30 min in blocking buffer (4% (wt/vol) UltraPure BSA (Thermo Fisher Scientific) in 2× SSC supplemented with 3% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were run in triplicate on a 4-12% Bis-Tris gel in 1X MES SDS running buffer for 40 minutes at 200V (all Invitrogen). Three biological replicates were run for each of the four groups ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were loaded in each lane and separated on a 4-12% Bis-Tris NuPAGE gel (Invitrogen), then transferred onto a PVDF membrane ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μl of this cell suspension was added as a spot in the center of a well in a 4-well chambered coverglass slide (Nunc). After a 1 hour incubation at room temperature to allow the cells to settle ...
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bioRxiv - Immunology 2022Quote: ... 2.5 μg of GPC or GPC-I53-50A was mixed with loading dye and run on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). For SDS-PAGE analysis ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... samples were washed twice in PBS and incubated in 0.05% trypsin containing 0.5mM EDTA (37°C, 4 min, Gibco Cat #: 25300054). Following trypsinization ...
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bioRxiv - Physiology 2022Quote: ... Incubation with blocking/permeabilization solution containing primary antibodies at 4 °C for overnight was followed by secondary antibody staining using suitable species-specific Alexa Fluor-coupled antibodies (Invitrogen) and flat-mounting in microscope glass slides with Fluoromount-G (0100-01 ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of the His6 tag was assessed by running “pre-thrombin” and “post-thrombin” samples on a 4-12% BOLT SDS PAGE gel in MES buffer (Invitrogen); the difference in size between the His6-tagged and untagged version was clearly resolved on these gels ...
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bioRxiv - Cancer Biology 2022Quote: ... The 4-micron Dionex IonPac AG-11-HC guard column (2 × 50 mm) and 4-micron IonPac AS11-HC analytical IC column (2.0 × 250 mm) were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The cDNA coding for fibulin1C (Uniprot number P23142-4) with a C-terminal 6x His tag was custom-synthesized by Invitrogen and transiently transfected in HEK293 T cells using polyethylenimine in OPTIMEM (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... purified PEP from chloroplasts was solubilized in Laemmli buffer and stacked in the top of a 4-12 % NuPAGE gel (Invitrogen). After staining with R-250 Coomassie Blue (Biorad) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibodies (diluted in 5% BSA and 2.5% NGS) overnight at 4°C followed by washing with 1X PBS and incubated with AlexaFluor (Thermo Fisher) conjugated secondary antibodies in the dark for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 19.5 µL were quenched by adding 7.5 µL 4 X LDS sample buffer (Life Technologies, Carlsbad, CA, USA), 3 µL 10X sample reducing agent (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... is prepared fresh each time by measuring NaIO4 powder and resuspending to a concentration of 4 mg/mL in aqueous solution of 200 mM Sodium Acetate pH 5.5 (Invitrogen, AM9740). Input RNA (up to 5 µg ...
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bioRxiv - Genetics 2022Quote: ... and a Qubit 4 Fluorometer with the Quant-iT™ Qubit™ dsDNA BR Assay Kit (Thermo Scientific, Wilmington, DE). DNA concentrations can be found in Table S2.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher). Plates were imaged using a 20x objective on a CellVoyager CV8000 High-Content Screening System (Yokogawa) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with 4 μg plasmid using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h culture ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 3.0-4.0 s at a blotting force of 4 and vitrified using a Vitrobot MarkIV (Thermo fisher Scientific) with chamber maintained at 8 °C and 100% humidity ...