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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Cells were lysed in RIPA buffer and 20 μg of protein lysate were separated on NuPAGE 4-12% Bis-Tris Midi Gels (Invitrogen). Proteins were transferred onto nitrocellulose membrane and stained with Ponceau Red to confirm complete transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Bleiswijk, the Netherlands) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... and HepG2 total RNA (4 µg) was input to reverse transcribe with the SuperScript III First-Strand cDNA Synthesis kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... spontaneous Ca2+ sparks were recorded from quiescent cardiomyocytes loaded with fluo-4 AM (20 μmol/L, Molecular Probes, Oregon, USA) and superfused with Hepes-Tyrode solution containing (in mmol/L) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded onto 4-well cover glass chambered dishes (Lab-Tek; 1064716) and transferred to Leibovitz’s L15 medium (Invitrogen; 11415064) for imaging ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The final concentration was 9.53 ng/μL measured with a NanoDrop 1000 spectrophotometer (Peqlab Biotechnologie, GmbH) and Qubit 4 Fluorometer (ThermoFisher Scientific) using 1X dsDNA HS Assay Kits ...
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bioRxiv - Genomics 2020Quote: 20μl of each cellular fraction was used for SDS-PAGE in a NuPAGE 4-12% Bis-TRIS gel and MOPs running buffer (ThermoFisher). For subcellular fraction marker proteins ...
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bioRxiv - Immunology 2021Quote: ... at 100% humidity and 4°C prior to plunge freezing into liquid ethane using a Vitrobot mark IV plunge freezer (ThermoFisher). For data set acquisition of apo NLRP3 ...
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bioRxiv - Genomics 2020Quote: Neuronal suspensions were labelled at 37°C with a combination of the Fluo-4 calcium indicator (5 μg/ml; Invitrogen) and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were stained for 25 mins at 4°C with LIVE/DEADTM fixable dead cell stains (Thermofisher Scientific) or DAPI (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were denatured in SDS-PAGE sample buffer and resolved in Novex™ 4-20% Tris-Glycine gels (Invitrogen) before immunoblotting with anti-ubiquitin or anti-ubiquitinated protein (clone FK2 ...
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bioRxiv - Molecular Biology 2021Quote: ... and centrifuged at 13,000 x g for 15 min at 4 °C and RNAs were lightly digested by treatment with MNase (Thermo Scientific) at a final concentration of 0.05 U μl-1 for 20 min at 16 °C ...
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bioRxiv - Immunology 2019Quote: Skin samples were collected from the cheek of mice at the indicated time points with a 4- or 6-mm biopsy punch into cold RPMI 1640 medium (RPMI; Gibco) and minced into smaller pieces with surgical scissors ...
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bioRxiv - Genetics 2019Quote: ... the lysate fraction was run on a NuPAGE 4-12% Bis-Tris gels (Novex) and transferred to PVDF membranes in an iBlot (Invitrogen). Membranes were blocked with 5% milk powder in TBS-T and incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Bioengineering 2019Quote: ... for 10 min at 98°C and were loaded onto pre-cast NuPAGE 4-12% (w/v) Bis-Tris 1.5-mm minigel (Invitrogen™). The electrophoresis was carried out at 180 V in NuPAGE MES SDS running buffer (50 mM Tris base ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.2% hydrogen peroxide in 0.5x SSC) and treated with proteinase K (4 μg/ml in 1x PBSTx, Thermo Fisher 25530049). Following overnight hybridizations at 56°C ...
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bioRxiv - Developmental Biology 2019Quote: ... equal amounts of otic vesicles or whole brain protein extract were resolved on NuPAGE 4-12% Bis-Tris Gels (Invitrogen) or NuPAGE 3-8% Tris-Acetate Gels (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... and 30 μg of protein was loaded onto a 4-12% Bis-Tris (Invitrogen/Thermo Fisher Scientific, Cat No. NPO323BOX), size fractionated at 200 V for 35 minutes ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation was performed as following: 25 µg of chromatin solution was incubated at 4° overnight with 25µl of the PrG-Dynabeads (Thermo Scientific),3µg of antibody (rabbit-anti Nucleolin ...
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bioRxiv - Plant Biology 2019Quote: ... The respective antibodies or IgG were coupled for 4-6 hour to Protein G Dynabeads (50µl, 10004D, Thermo Fisher Scientific) and subsequently incubated overnight with equal amounts of sonicated chromatin ...
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bioRxiv - Genomics 2019Quote: ... Samples were then incubated overnight at 4°C with 1ug of H3K9me3 antibody and 10ul of Dynabeads (Life Technologies 1006D). Libraries were then prepared using the BIOO NextFlex sequencing kit and sequenced at the Vincent J ...
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bioRxiv - Immunology 2019Quote: ... for 30 minutes and then concentrated onto a poly-lysine coated slide using the Cytospin 4 cytocentrifuge (Thermo Fisher Scientific), for 5 minutes at 1250 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... 24 h after the addition of VEGF-A cells were fixed with 4 % PFA before further processing according to the Click-iT EdU Imaging Kit (Invitrogen). Experiments were performed with eight replicates per group ...
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bioRxiv - Microbiology 2019Quote: ... The samples were centrifuged at 10,000 x g for 2 minutes and 10 µl portions of the supernatants were loaded on a 4-12% Tris-Glycine slab gel connected to Tris-Glycine SDS running buffer (Invitrogen). The gel was electrophoresed at 200 volts for 1 hour ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were boiled and reduced in a mixture containing NuPAGE® Sample Reducing Agent (10X) and 4X Protein Loading Buffer before being resolved by SDS gel electrophoresis on 4-20% Tris/glycine gels (Invitrogen). Proteins were transferred onto a nitrocellulose membrane utilizing a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... 10-15% of RIPA-soluble fraction and 2.5-5% of insoluble fraction were loaded on 4-12% Bis-Tris gels and run using MOPS buffer (Life Technologies) and visualized by western blotting on PVDF ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were then clarified through centrifugation at 10000 rpm for 10 min at 4°C and concentration determined measuring Absorbance at 280nm in the Nanodrop 1000 (ThermoFisher). Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated with appropriate secondary antibodies and incubated for two to three hours at room temperature or overnight at 4°C (goat anti-rabbit IgG 488 or 647; Invitrogen, A11034 and Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Biochemistry 2019Quote: ... cell numbers were determined by trypsinating and counting cells from 4 wells using a Countess automated cell counter (Thermo Scientific). The experiment was done in triplicates ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Biophysics 2019Quote: 100-nm diameter tetraspeck fluorescent beads samples (Supplementary Fig. 4) were prepared by diluting the initial solution (T7279, Thermo Fisher) at 5.10−4 in PBS + 5% glucose ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes and separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris Gel (ThermoScientific) in 1x Bolt MES SDS Running Buffer (Invitrogen) for 60 minutes at 130V.
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bioRxiv - Microbiology 2019Quote: ... Samples were heated in Laemmli buffer at 100°C for 5min before proteins were separated on gradient polyacrylamide gels (4-12%, NuPage, Invitrogen), which were run at 175V for 50min ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were separated on a precast 4-12%Bis-Tris Plus gel in Bolt™ MOPS SDS running buffer (Invitrogen). Gels were blotted on a Nitrocellulose membrane (Amersham TM Protran™ 0.45um ...
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bioRxiv - Physiology 2019Quote: Sensory nodose neurons isolated from B6.129F and TRPA1-deficient mice were loaded with Flou-4 NW with pluronic acid F-127 (Molecular Probes) for 60 minutes at 37°C for 45 min in neurobasal-A medium ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifugation at 14 000 xg for 15 min at 4°C (Heraeus Multifuge X3R; Thermo Fisher Scientific, Vienna, Austria) the supernatant was filtrated through a 0.8 μm strainer (VWR ...
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bioRxiv - Bioengineering 2019Quote: ... Proteins were loaded on 4-20%Tris-Gly gradient gels (invitrogen XP04200BOX) then transferred to nitrocellulose membranes with a dry transfer system (Invitrogen iBIot Blotting System and Gel Transfer Stacks Invitrogen IB301001) ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were separated on a 4–12% gradient Bis-Tris gel (NuPAGE Bis-Tris gels, 1.0 mm, 10 well; Art. No. NP0321; Life Technologies) in 1X MOPS (NuPAGE 20X MOPS SDS running buffer ...
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bioRxiv - Cell Biology 2019Quote: ... specimens were incubated with primary antibodies at room temperature for 1 hr or at 4°C overnight and then stained with a mixture of an Alexa Fluor 488-conjugated antibody (Invitrogen) (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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bioRxiv - Cancer Biology 2019Quote: ... and samples were denatured at 70C for 10 minutes in NuPAGE LDS sample buffer with NuPAGE sample reducing agent and then resolved on a 4-12% Bis-Tris gradient gel (Invitrogen). For chemiluminescent detection ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were centrifuged (15,000 xg, 30 min 4 °C) and the supernatants mixed with 30 μl of NeutrAvidin agarose beads (Thermo Scientific) for 2 hrs at 4 °C to adsorb the biotinylated proteins ...
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bioRxiv - Pathology 2020Quote: ... Reverse-phase mobile buffers were composed of A: 0.1% (v/v) FA (Thermo Scientific, MA, US, Cat No. 34851-4), and B ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... 4 × 106 HEK293T cells were seeded the day before transfection in 100 mm dishes in DMEM medium (Gibco Life Technologies) with 10% fetal bovine serum (FBS ...