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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fetuses and placenta were flash frozen in dry ice or fixed in 4% paraformaldehyde (Thermo Fisher Scientific #J19943.K2) for 72 hours at 4°C for immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2024Quote: ... and infected with sendai virus (SeV) carried polycistronic Klf4-Oct3/4-Sox2 and Klf4 vectors (ThermoFisher, Waltham, MA, #A16517), here referred as OSK-SeV ...
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bioRxiv - Physiology 2024Quote: ... boiled at 95°C for 5min and electrophoresed on BoltTM 4-12% Bis-Tris Plus gel (Thermo Fisher, NW04122BOX) at 200V for 20min ...
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bioRxiv - Cell Biology 2024Quote: ... 1200 µg of protein was incubated overnight rotating at 4°C with 200 ul of Streptavidin beads (Thermo Scientific) which were acetylated with Sulfo-NHS-Acetate beforehand ...
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bioRxiv - Cell Biology 2024Quote: ... (Pierce) were added to 4-15% polyacrylamide gradient gels and supplemented with Coomassie Brilliant blue (0.2% final concentration; ThermoFisher) added to the supernatant ...
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bioRxiv - Genomics 2024Quote: ... The concentration and quality of the libraries were evaluated using a Qubit 4 fluorometer (Thermo Fisher Scientific, MA, USA), 2100 Bioanalyzer System (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... bronchial tissues were rinsed and then incubated overnight at 4°C with MEM medium (Life Technologies, Burlington, QC, CA) supplemented with 7.5% NaHCO3 (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and GFP+ transduction was immunoenhanced using an anti-GFP 488 antibody (ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation and were resolved with NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) prior to transfer onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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Self-assembled DNA-collagen bioactive scaffolds promote cellular uptake and neuronal differentiationbioRxiv - Bioengineering 2024Quote: ... The cells were then labeled with 4 µg/mL goat anti-rabbit (For Integrin-Beta and Vinculin: Invitrogen; A11011) or anti-mouse (For MAP2 ...
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bioRxiv - Bioengineering 2024Quote: ... The islets were subsequently incubated at 4°C overnight with anti-mouse Alexa Fluor 488 (Invitrogen, cat. no. A28175) and anti-rabbit Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... on 4 ng of cDNA in a 10 µL reaction consisting of SYBR green master mix (Applied Biosystems #4367659) with 0.3 µM forward and reverse primers (Table 1).
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated in PBS with 4% EDTA supplemented with DAPI and fluorescein diacetate (FDA, Thermo Fisher F1303) at 37 °C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatants were isolated by centrifugation at 12,000 × g at 4°C for 30 min and then mixed with 30 μl of Protein G Dynabeads (Invitrogen) that had been preincubated with antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μg of sample was then loaded onto an SDS PAGE pre-cast 4-12% Bis Tris gel (ThermoFisher) and separated with MOPS buffer (ThermoFisher) ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Formalin fixed paraffin embedded (FFPE) tissue sections (4 μm) were prepared on Superfrost Plus microscope slides (Thermo Fisher Scientific). Sections were deparaffinized followed by antigen retrieval in CC1 buffer (pH 9 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 4 minutes at 37°C with a 100-fold dilution of 0.05%Trypsin-EDTA solution (Gibco), next cells were fixed as above ...
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bioRxiv - Biochemistry 2024Quote: Gel electrophoresis was performed at 195V for 65 min in Novex 4-20% Tris-Glycine gels (WXP42020BOX, Thermo Fisher). For western blotting ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated using SDS polyacrylamide gel electrophoresis (Bolt™ 4-12% Bis-Tris Plus Gel, Thermo Fisher Scientific) and further transferred onto a PVDF membrane (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Protein lysates were separated using 4-12% Bis-Tris SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Thermo Fisher Scientific, #NP0321) for 1-2 h at 150V ...
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bioRxiv - Cell Biology 2024Quote: ... Metabolite separation was performed using a Dionex IonPac AS11-HC column with 4 μm particle size (Thermo Fisher Scientific). The flow rate for IC was set at 0.25 mL/min ...
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bioRxiv - Plant Biology 2024Quote: ... 40 µL of each sample was loaded onto stain-free gradient gels (4–20% Precise Protein Gels, Thermo Scientific) and separated at 150 V for 1 h in 1X TBS electrophoresis running buffer (24.8 mM Tris base ...
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bioRxiv - Plant Biology 2024Quote: ... 100 ng of RNA were prepared in equal volumes (4 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Molecular Biology 2024Quote: ... RNP complexes attached to the beads were subjected to treatment with DNase (Turbo DNase [Ambion], 4 U/100 μL) and optionally RNase I (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was incubated overnight at 4°C with anti-His-Tag antibodies (Thermo Fisher Scientific, USA, #MA1-21315) diluted 1:1000 in the blocking solution ...
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bioRxiv - Genomics 2024Quote: ... and DNA concentrations measured with the dsDNA High Sensitivity Assay kit on a Qubit 4 Fluorometer (both Invitrogen, USA) using 2µL of DNA extract as input ...
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bioRxiv - Immunology 2024Quote: ... cell culture plates were harvested and stained for the presence of SSEA-4 (clone MC813-70, Thermo Fisher Scientific) to characterize pluripotency per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 5 000×g for 5 min at 4°C in SpinX 0,2 µm tubes (Corning, Fisher Scientific) for cell debris and bacterial removal ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was replaced with 5 mM glucose-containing RPMI 1640 with Di-4-ANEPPDHQ (Thermo Fisher Scientific) at 2 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed in 4% paraformaldehyde for 15 min at room temperature and permeabilized with 0.1% Triton X-100 (Cat# 85111, ThermoFisher) for 30 min in PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... frozen at -20°C and resuspended in 4 mL/gr of pellet of B-PER Reagent (Thermo Fisher Scientific) with 2 µl of lysozyme (50 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... The LOS extracts were then run on NuPAGE Bis-Tris gradient (4-12%) SDS-PAGE gels (Invitrogen, Waltham, USA) with NuPAGE MES running buffer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: T24 human epithelial bladder cells (ATCC HTB-4) were grown in McCoy’s 5A modified medium (Gibco 16-600-082) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Conductivity was measured using a Thermo Scientific Orion 4-Star Benchtop pH/Conductivity Meter (Thermo Fisher Scientific, Massachusetts, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Isolated pericentral/periportal hepatocytes were rocked for 10 min at 4 °C with RIPA lysis buffer (ThermoFisher Scientific, #89900) with protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... for 10m and blocked overnight by using blocking solution (PBS plus 4% bovine serum albumin, fraction V, Fisher Scientific 9002-46-8 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: DNA extractions were quantified using Qubit 4 fluorometer with a double stranded DNA broad range quantification assay (ThermoFisher, UK) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg protein were loaded onto 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... Time points were resolved by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Biochemistry 2020Quote: ... AB_2617216) was added to sections overnight at 4°C followed by Alexa Fluor®-conjugated goat IgG against mouse IgM (Invitrogen, Carlsbad, CA, 1:500 dilution), for 40 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated overnight with primary antibodies at 4°C followed by incubation with appropriate secondary antibodies (1:1000; Invitrogen Alexa Fluor™ 488/594/647) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Cxcr3a::GFP samples were incubated with primary antibodies against GFP for 48 h at 4°C (chicken anti-GFP (Thermo Fisher Scientific, Cat#: A10262; 1:300)) ...