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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Gel electrophoresis was performed using 4-12% NuPAGE Bis-Tris gels according to manufacturer’s protocols (NuPAGE system, Thermo Fisher Scientific). Protein gel bands were excised ...
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bioRxiv - Genetics 2021Quote: ... The aqueous layer was transferred to a new tube and 500ul of 4° isopropanol with 1ul Glycoblue (Thermo Fisher Scientific) was added to precipitate the RNA ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Immunology 2021Quote: ... All fixed/permeabilized cells were washed three times in room temperature Perm/Wash buffer and then stained in 2mL of Perm/Wash buffer with 4 drops of the NucBlue Fixed Cell ReadyProbes reagent (ThermoFisher) for 15 minutes at room temperature ...
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bioRxiv - Genetics 2019Quote: ... by incubating at 70°C for 10 minutes and separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) along with 5% of input samples and analysed by Western blotting as described above.
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bioRxiv - Evolutionary Biology 2021Quote: ... The in situ hybridization experiments were carried out on 4 µm sections mounted on SuperFrost Plus™ Slides (ThermoFisher Scientific) using the RNAscope 2.5 HD Detection Reagent RED kit according to the manufacturer’s recommendations (Advanced Cell Diagnostics) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell debris was pelleted by centrifugation at 13,000g for 10 minutes at 4°C and the protein concentration measured by bicinchoninic acid (BCA) assay (Pierce, Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... for the β-tubulin samples and 4 ng/µl of the multiple-labeled goat anti-rabbit IgG secondary antibody DOL 8.3 (Invitrogen, 31212) for the clathrin samples for 45 min ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos/hearts were then washed with 1% BSA/PBSTX and incubated for 48 hours at 4°C in goat anti-rabbit Alexa Fluor 568 (embryos) (Cat. No. A-11036; Invitrogen) or goat anti-rabbit Alexa Fluor 488 (hearts ...
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bioRxiv - Genomics 2020Quote: Total protein from equal amounts of cells was separated on a NuPage Gradient 4-12% Bis-Tris Mini Gel (Invitrogen), transferred to a PVDF membrane ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 4 µg DNAse-treated total RNA using Superscript II or IV Reverse Transcriptase (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... hippocampal tissue from E19 Sprague Dawley albino rat embryos was harvested on ice in 4°C Hibernate E (Life Technologies) and digested with 20 units/mL papain (Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at 3000 × g at 4°C for 30 mins and subsequently lysed with B-PER (Thermo Scientific) supplemented with 50 U DNAseI ...
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bioRxiv - Neuroscience 2021Quote: ... 25 µl of supernatant was mixed with loading buffer and resolved by SDS-PAGE in 4- 12% Bis–Tris gels (Invitrogen) under reducing conditions and electro-blotted onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately 20 μg of protein per sample was loaded into a NativePAGE Novex 4-16% Bis-Tris polyacrylamide gel (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... were plated in 24-well plates at 4×104 cells per well in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Thermo Fisher, USA) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... The immunoblots were analyzed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA was used to quantify Taqman probes (target-FAM and housekeeping-TET) provided in Supplementary Table 4 and TaqMan Fast advanced Master Mix (ThermoFisher) on a 7900 Fast Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 450 xg for 10 min at 4 °C and pellets were incubated with red blood cell lysis buffer (Invitrogen) for 30 sec ...
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bioRxiv - Cell Biology 2020Quote: ... Laemmli sample buffer (0.1 mL of 4x) was added to lysate prior to loading 10 μL on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). Gels were transferred onto a Mini-size polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were desalted into reaction buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl and 4 mM Tris (2-Carboxyethyl) Phosphine (TCEP) (Invitrogen, T2556)) for removing DTT in storage buffer ...
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bioRxiv - Cell Biology 2021Quote: ... WCE and bound proteins were separated by running in a 4-12% Bis-Tris gradient gel with MOPS SDS running buffer (Invitrogen). Mif2-TAF and Flag-Cse4 were detected by immunoblotting with anti-protein A (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (10-20 μg) were run on SDS/PAGE with NuPAGE precast 4-12% gradient gels (Invitrogen; Carlsbad, CA, USA) and blots were incubated overnight at 4°C with primary antibodies (Supplementary Table 1 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein fractions were subjected to SDS-PAGE analysis and the fractions containing purified TtArsM were pooled and dialyzed for 16 h at 4°C in 20 mM NaP pH 7.4 buffer supplemented with protease inhibitor cocktail (Thermo Scientific). The identity of the purified His-tagged TtArsM protein was confirmed by mass spectrometry and protein aliquots were stored at -20°C.
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates containing 10 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Brain extracts containing 25 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Biophysics 2022Quote: ... and CpE with C-terminal decahistidine tags preceded by thrombin cleavage sites (claudin-4-His10, cCpE-His10, and CpE-His10) were cloned into pFastBac1 (ThermoFisher) and expressed in Tn5 (Trichoplusia ni ...
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bioRxiv - Biochemistry 2020Quote: Purified rFIX samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-12% Bis-Tris Bolt acrylamide gel (Invitrogen) in 1x MES running buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... 15 or 35 µl/well) of fresh media (stored in a regular 4 °C fridge, oxygen saturated) with and without the hypoxia dye (Invitrogen, Image-iT Green Hypoxia Reagent ...
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bioRxiv - Bioengineering 2021Quote: ... cocultured with macrophages in RPMI +10%FBS for 48 h and fixed afterwards for imaging using 4% paraformaldehyde (PFA, ThermoFisher). Cells were imaged using a DMI 6000B inverted phase contrast microscope (Leica Camera ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were washed with PBS and incubated overnight at 4° C with blocking buffer (Thermo Scientific, Waltham, MA, cat. #37572) to eliminate nonspecific sites ...
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bioRxiv - Bioengineering 2020Quote: ... The next day samples were centrifuged at 13,000 rpm for 10 min at 4 °C (Thermo Scientific Sorvall Legend X1R) The supernatant was removed and placed in a speed-VAC (Savant SC110 ...
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bioRxiv - Neuroscience 2021Quote: ... the beads bearing the immunoprecipitated products were washed four times with IP buffer at 4° C and eluted with 50μl of 2X LDS loading buffer (Invitrogen #NP0007) at 55° C for 30 min ...
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bioRxiv - Bioengineering 2020Quote: Equal masses of protein lysate or equal volumes of immunoprecipitation eluents were loaded into NuPAGE 4-12% Bis-Tris gels (ThermoFisher). After electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 minutes at 70°C and then loaded into a 4-12% NuPAGE Bis-Tris gel (Invitrogen, NP0326BOX). After electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... and samples were incubated at 70°C for 5 minutes and loaded onto Bis-Tris 4-12 % gradient gels (ThermoFisher). Bands were stained by CBB and relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: Scc2/4 was labeled by mixing the protein in an equimolar ratio with Qdot 705 Strep Conjugate (Thermo Fisher Scientific) and incubating for 10 min on ice in BSA buffer (40 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: Protein samples in 1X SDS loading buffer/100 mM dithiothreitol (DTT) were resolved by gel electrophoresis on acrylamide NuPAGE Novex 4-12% Bis-Tris Protein Gels (Invitrogen) with NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...
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bioRxiv - Neuroscience 2020Quote: ... 10-30 μg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... blood was removed with 0.9% saline followed by perfusion and fixation with 4% chilled paraformaldehyde (PFA, Fisher Scientific, Pittsburgh, PA). Perfused brains were post-fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to electrophoretic separation on a 4-20% Tris-Glycine polyacrylamide gel (ThermoFisher Scientific, #XP04200), transferred to Nitrocellulose membrane (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.