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Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 9 µM Bodipy (Thermo Fisher Scientific) for lipid droplets ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 µL of M-280 Streptavidin Dynabeads (Invitrogen) per time point were washed 3 times with 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: A real-time TaqManTM assay was performed using the Applied-Biosystems 7500 real-time PCR system (ThermoFisher Scientific). WNV primer/probe mix (Forward-primer sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... recombinant Cas9 protein (100 ng/μl; GeneArt Platinum Cas9 Nuclease, Thermo Fisher Scientific, Waltham, MA, USA), and ssODNs (400 ng/μl ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were mounted with ProLong(™) DiamondAntifade Mountant with DAPI (Fisher Scientific, P36971), visualized with Axio Imager 2 microscope using 5×/0.15 N-Achroplan or 20×/0.5 EC Plan-Neofluar objectives ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated with 15 mL ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) for 1-2 hours at 42°C ...
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bioRxiv - Molecular Biology 2024Quote: ... using Fast SYBR Green Master Mix (Applied Biosystems: 4385612). 0.2 μL each of 50 μM forward and reverse primers were combined with 4 μL of the treated sample ...
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bioRxiv - Molecular Biology 2024Quote: ... and supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1× penicillin–streptomycin (Thermo Fisher Scientific, 15140122) at densities from 10–20 k/ well in cell-culture coated 96 well plates (Corning Costar ...
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bioRxiv - Molecular Biology 2024Quote: ... sodium pyruvate and GlutaMAX (Thermo Fisher Scientific, 35050079) and supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2024Quote: ... 5 μL of 100bp DNA ladder (SM0243, Thermo Fisher Scientific, USA) was added and 10 μL PCR products were added in the remaining wells along with positive and negative control ...
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bioRxiv - Microbiology 2024Quote: Total RNA from saliva was extracted with MagMAX CORE Nucleic Acid Purification kits (Applied Biosystems, Foster City, CA). Micro-RNAs were detected using Illumina Hiseq x Ten (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... and DNase I (Thermo Scientific, EN0521) at room temperature for a 1h.
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) with HaltTM protease and phosphatase inhibitor cocktail (Thermo Scientific, 78441). The suspension was gently pipetted until no visible pellet ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA samples were isolated from human hippocampus and cerebellar tissues using TRIzole (Life Technologies), as we reported 68,69 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1X non-essential amino acids (Gibco). On day of analysis ...
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bioRxiv - Cell Biology 2024Quote: Co-immunoprecipitation for the MeCP2-Flag and hnRNP L-HA interaction was performed using a Pierce™ Co-IP Kit (Thermo Scientific, 26149) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and BglII (#ER0082, Thermo Fisher Scientific, US), fractionated in 1% agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... GH3 cells were transduced with supernatants containing virus carrying the shMeCP2 or shhnRNP L construct using the Lipofectamine 3000 reagent (Invitrogen). After 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Barcoded wells from the 2nd-round NGS product were pooled by experiment and loaded onto E-gel EX 2% Agarose gels (Invitrogen, G401002) for visualization ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mixture contained 10 μL platinum green hot start PCR master mix (13001014, Invitrogen, USA), 1 μL of forward primer ...
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bioRxiv - Molecular Biology 2024Quote: ... which were then washed with 100 μL of 1X PBS pH 7.4 (Gibco, 10010023). The PBS was then removed and 50 μL of a lysis buffer added ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting products were then recombined into pDONR221 (Thermo Fisher Scientific, cat # 12536017) through a Gateway BP reaction with Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and MkPs were purified by flow cytometry and EdU incorporation analyses were performed according to the manufacturer’s protocols (Click-iT® EdU Flow Cytometry Assay Kits, Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... through a Gateway BP reaction with Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, cat # 11789020) to produce pDONR221-p24 entry clones ...
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bioRxiv - Cell Biology 2024Quote: ... p24 sequences were transferred into modified Gateway destination vector pTSGW (UASt-Signal peptide of Tango1-GFP-Gateway cassette) (Yang et al., 2021) through Gateway LR recombination using LR Clonase II Plus enzyme (Thermo Fisher Scientific, cat # 12538120) to obtain the desired plasmids.
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bioRxiv - Microbiology 2024Quote: ... Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes, Life Technologies), and qualification was done using the Agilent D1000TS Kit and Tapestation 4200 ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 U of DreamTaq polymerase (Fisher Scientific, Waltham, MA) and 1 μl template DNA (McManamon et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from all experiments was reverse-transcribed to cDNA using the RevertAid cDNA prep kit (K1621, Thermo Fisher Scientific). For qPCR assays and higher abundance genes ...
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bioRxiv - Molecular Biology 2024Quote: ... and 40 ng of Cas7-11 expressing plasmid were cotransfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) according to manufacturer standards.
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bioRxiv - Molecular Biology 2024Quote: ... sodium pyruvate and GlutaMAX (Thermo Fisher Scientific, 35050079) and supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2024Quote: ... washed once with 1X PBS (Gibco, 10010023) and replaced with 100 μL (for 24 wells) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1X Protease Inhibitor Cocktail (Thermo Fisher Scientific, 78429). Cells were lysed on ice for approximately 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes, Life Technologies), and qualification was done using the Agilent D1000TS Kit and Tapestation 4200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were transferred to nitrocellulose membranes via an iBlot-2 transfer device (Thermo Fisher Scientific), blocked for 1h at RT using Intercept PBS LI-COR Blocking Buffer (LI-COR ...
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bioRxiv - Molecular Biology 2024Quote: HEK293FT cells (Invitrogen, R70007) were cultured in Dulbecco’s modified Eagle medium with high glucose ...
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bioRxiv - Molecular Biology 2024Quote: ... and supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1× penicillin–streptomycin (Thermo Fisher Scientific, 35050079). Cells were maintained at 37 ℃ and 5% CO2 throughout all experiments.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was collected and concentration determined by Pierce BCA assay (Thermo Fisher Scientific 23227). Concentrations were normalized and equal amounts from each sample run on Bio-Rad 4–20% Mini-PROTEAN gels (4561094 ...
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bioRxiv - Microbiology 2024Quote: ... along with SYBRTM Safe DNA Gel Stain (S33102, Invitrogen, USA). In the first and last wells of the gel ...
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bioRxiv - Cell Biology 2024Quote: ... Limited studies were carried out using media-2 but was prepared using Advanced DMEM/F12 (Gibco) supplemented with 1% B27 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lipofectamine LTX with PLUS reagent (Invitrogen by Thermo Fisher Scientific) was used to transfect the ONs according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... at a final concentration of 100 or 200 nM in OptiMEM reduced serum medium (Gibco). For gymnosis experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... and penicillin-streptomycin (10,000 U/mL, Gibco™, Waltham, MA, USA). The cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2024Quote: ... with 10% fetal bovine serum (Gibco™, Waltham, MA, USA) and penicillin-streptomycin (10,000 U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in DMEM (Dulbecco’s Modified Eagle Medium) with pyruvate and low glucose (Gibco), supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Lipofectamine LTX with PLUS reagent (Invitrogen by Thermo Fisher Scientific) was used to transfect the ONs according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... using iBlot2 Gel Transfer Device (Invitrogen). The membranes were blocked with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Molecular Biology 2024Quote: Relative expression levels of the target GFP gene was assessed by qRT-PCR analysis using the SYBR™ Select Master Mix (Thermo Scientific™). The β-actin housekeeping gene was used as an internal control for the normalisation of gene expression.
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bioRxiv - Molecular Biology 2024Quote: ... TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific). HEK293 cell lines with tetracycline inducible TAP-tagged baits were generated by co-transfection of 0.1 µg of plasmid containing the TAP-tagged bait (hsINTS13ΔC ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were transferred onto nitrocellulose membranes (Invitrogen) using iBlot2 Gel Transfer Device (Invitrogen) ...