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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The denaturing effect of SDc was quenched by addition of 0.8 μL of 1:2500,000 diluted ERCC RNA Spike-In Mix 1 and 2.8 μL of quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]), followed by incubation at 72°C for 90 sec ...
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bioRxiv - Immunology 2023Quote: ... peptides were first loaded onto a trap cartridge (ThermoFisher PepMap, C18, 5 μm, 100 μm i.d. x 20 mm), then eluted onto a reversed phase Easy-Spray column (ThermoFisher PepMap ...
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: The collected tissue was quickly washed in PBS twice and centrifuged for 5 minutes at 0.2 x g prior to dissociation with 500 μL of TrypLE Express (Gibco) at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of each restriction digest reaction was combined with 1 μL 6X TriTrack DNA Loading Dye (Thermo Fisher) and run on a 2% agarose gel pre-stained with ethidium bromide ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Two rounds of gravity settling were performed before plating the cells on laminin (5 μg/ml in PBS, ThermoFisher) coated plates with addition of DMEM/F12 with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... and the extraction was repeated with 50 % ACN / 5 % Formic Acid (HCOOH) (LC-MS Grade, #85178, Thermo Fisher Scientific). The solution was dried in vacuum centrifugation for 2 hours 30 minutes (maximum vacuum pressure up to 5.1 ...
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bioRxiv - Biophysics 2023Quote: ... immunostained cells were exposed for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific, Waltham, Massachusetts) in water.
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bioRxiv - Developmental Biology 2023Quote: ... 1×3–5 min with 1x PBS + 1 ng/mL DAPI and mounted in ProLong Gold antifade reagent (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells were seeded in a 100 mm dish and selected with 5 µg/ml Blasticidin S HCl (Invitrogen). Colonies formed after 2-3 weeks were analyzed for anti-TET repressor by western blots (data not shown) ...
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bioRxiv - Biochemistry 2023Quote: ... at 37°C and under 5% CO2 atmosphere in a HERA cell VIOS 160i CO2 incubator (Thermo Fisher Scientific). The cells were passaged using 1× TrypLETM Express (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... The 5 framework sequences adjacent to the targeted CDRs were amplified using Phusion High-Fidelity DNA polymerase (Thermo Scientific). Framework fragments were assembled with the neighboring mutagenized CDR fragments by sequential ...
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bioRxiv - Microbiology 2023Quote: RK-13 rabbit kidney cell line obtained from the ATCC (ATCC CCL-37) was grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco), containing 10% FBS (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... incorporated 5-EU was detected by the Click-iT™ RNA Alexa Fluor 594 Imaging Kit (ThermoFisher, cat: C10330). Intracellular antibody labelling was performed after the Click reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Immunology 2022Quote: ... cells and cellular debris were removed by centrifugation at 4,000 × g for 5 minutes followed by filtration through a 0.22 μm Nalgene rapid-flow filter (ThermoFisher Scientific). Clarified supernatant was incubated with His60 Ni-Superflow resin (Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were cultured at 37 °C in a humidified atmosphere at 5% CO2 with Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 1 hour at room temperature and subsequently blocked with 5% bovine serum albumin (ThermoFisher Scientific 37525) and 10% donkey serum (Lampire 7332100 ...
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bioRxiv - Immunology 2023Quote: ... Confluent TeloRF cells were infected with 1 MOI UCD52 RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Quantitative real-time PCR was conducted using SYBR Green chemistry on a Quant Studio-5 instrument (Applied Biosystems, USA). The reaction volume of 20 µL consisted of 10 µL of TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Genetics 2023Quote: ... 5 µg of purified total RNA was then treated with DNase (TURBO DNA-free kit, Thermo Fisher (MA, USA) according to manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 25,000 cells were plated onto 24-well plates that were coated with fibronectin (5 µg/cm2, ThermoFisher). To evaluate the effects of exogenous TGFβ ligand or inhibition of TGFβ signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... and p-Smad1/5/8 and thoroughly analyzed under a fluorescent light microscope (EVOS FL, Life Technologies, Carlsbad, CA). Single-plane laser scanning confocal photomicrographs were taken from every other section using a laser scanning confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Immunology 2023Quote: ... we assembled RNP complexes by thoroughly mixing 2.25 ul of 5 ug/ul TrueCut Cas9 Protein V2 (Thermo Fisher) and 5ul 40uM gRNA duplex and incubating at RT for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... for 2 h at 37°C at 5% CO2 in the presence of 0.5 mg/ml dextran-rhodamine (MW=10,000, Molecular Probes). To test sporozoite hepatocyte traversal in the absence or presence of anti-CSP mAbs ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Neuroscience 2023Quote: ... After addition of protein G-coated magnetic dynabeads (5 μl beads/μg antibody, Thermo Fisher Scientific, cat. no. 13424229), the lysate+antibody+bead mixtures were incubated for 1 hour at 4°C with end-over-end mixing ...
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bioRxiv - Cell Biology 2023Quote: ... Chondrocytes were collected and cultured in an incubator containing 5% CO2 at 37°C with Dulbecco’s DMEM/F12 (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco) and 100 U/ml penicillin G sodium ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed 5 times with wash buffer and 2 times with 5x SSC (Gibco ref 15557-044) + 0,1% Tween 20 ...
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bioRxiv - Developmental Biology 2023Quote: ... Separation of metabolites was achieved in 12 minutes using a Hypercarb column (5 mm, 100X2.1 mm column, Thermo Scientific) and a gradient run of solvent A (7.5 mM ammonium acetate with 0.05% ammonium hydroxide ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4) and then each well was loaded with a cell-permeant 5 μM Fluo-4AM solution (ThermoFisher Scientific) for 40 minutes ...
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bioRxiv - Biophysics 2023Quote: ... HeLa and MDCK cells were cultured at 5% CO2 and 37°C in Dulbecco’s modified Eagle medium (DMEM, Capricorn) with 1% Penicillin Streptomycin (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (FBS) low in endotoxins and heat-inactivated (Life Technologies, Inc., Rockville, MD, USA) and incubated at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3 nM of fluorescently labeled and biotinylated DNA (or 2x1.5 nM with experiments for the intermolecular cases) were bound to 5 nM Streptavidin in PBS and with 1:1800 Tetraspeck beads (0.1 µm, ThermoFisher). After ∼10 min incubation time ...
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bioRxiv - Plant Biology 2023Quote: ... the supernatant containing the soluble fraction was loaded onto a Pierce 5-mL Ni-NTA column (Thermo Fisher Scientific) equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to ultraviolet light (UV) for 5 minutes with a 2% of sulfa SANPAH solution (Thermo scientific, 22589). The hydrogels were washed several times under sterile conditions and incubated with 3.3% of Rat Tail Collagen 1 (Gibco A10483 ...
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bioRxiv - Cell Biology 2023Quote: ... The bottom chamber was filled with 5 ml cartilage medium consisting of α-MEM (41061, GibcoTM, Thermo Fisher Scientific), 10% v/v FBS (BCBV7611 ...
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bioRxiv - Molecular Biology 2023Quote: ... except that the purification on sucrose cushion was performed before the digestion with RNase I (Ambion, 5 units/UA260). Biotinylated oligonucleotides (IDT - Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 2-5 µg of the indicated plasmid using Lipofectamine 3000 as per manufacturer’s instructions (ThermoFisher). Transfection medium was exchanged to fresh medium and cells were moved to 10 cm plates 4-6 hours post-transfection ...
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bioRxiv - Plant Biology 2023Quote: ... 30 ng of the cDNA were used for RT-qPCR analysis (QuantStudio 5 Real-Time PCR system; Applied Biosystems). Ct values were analyzed with QuantStudio design and analysis software (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured as neurospheres at 37 °C with 5% CO2 in NPC medium: DMEM/F12 (Gibco, CAT# 10565018) with GlutaMAX supplement ...
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bioRxiv - Immunology 2023Quote: ... Amplification and cloning of the truncated versions of TGM4 (D1-3 and D4-5) was performed by PCR amplification using proofreading Taq polymerase Phusion Hi as per the manufacturer’s instructions (Invitrogen), full-length codon-optimised TGM4 as template ...
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bioRxiv - Developmental Biology 2023Quote: ... Human immortalised endometrial epithelial cells (Ishikawa cells) were cultured at 37°C (5% CO2) in DMEM:F12 B-Nut mix (50:50, ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: NIH/3T3 mouse embryonic fibroblast cells (ATCC) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) (ThermoFisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the tubules were digested using 10 ml of DMEM-F12 buffer containing 5 mg of trypsin (Thermo Fisher 27250018) on a 50 ml conical tube and the reaction was carried out on a 35°C shaker water- bath ...
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bioRxiv - Cell Biology 2023Quote: ... 5 × 105 cells/ml were plated in three 50 mm non-treated tissue-culture dishes (122-17, Thermo Scientific). For control ...
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bioRxiv - Cancer Biology 2023Quote: ... and the mouse macrophage cell line RAW 264.7 were cultured at 37°C in 5%CO2 in RPMI-1640 medium (Invitrogen) containing 10% (vol/vol ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.