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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the PCR products were directly labeled with Alexa Fluor 555 dye and purified using the FISH Tag DNA Orange Kit (ThermoFisher Scientific). Labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The standard phenol/chloroform (ThermoFisher, 10308293) process was used to extract DNA for the following qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBS with 1% TWEEN-20 (Fisher Scientific, BP337-100) (PBST ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 205 g/L AlbuMAX I Lipid-Rich BSA (Thermo Fisher 11020039), 15 mg/L hypoxanthine (Sigma H9636) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with anti-AKAP12 (Thermo Fisher #PA5-21759, 1:200), anti-βIII-Tubulin (R&D Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 μL Proteinase K (10 mg/ml) (Thermo Scientific™, EO0491) were added to sample tube and incubated at 65°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μL Proteinase K (10 mg/ml) (Thermo Scientific™, EO0491) were used and incubated at 55°C for 1h with vigorous pipetting every 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... using Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher 23400021) supplemented with 205 g/L AlbuMAX I Lipid-Rich BSA (Thermo Fisher 11020039) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proper secondary antibodies were used for 1h at room temperature before visualised the membranes with Pierce™ ECL Western Blotting Substrate (Thermo Scientific, 10005943) or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... or SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, 34580) and Amersham™ Hyperfilm™ ECL™ (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were precleared with 25 μL/sample Protein A Dynabeads (Life technologies, 10002D) for 1h before incubated with 2.5 μg S9.6 antibody (sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... based on the SeeBlue2 plus molecular weight ladder (Thermo Fisher Scientific), were excised with a clean scalpel ...
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bioRxiv - Molecular Biology 2024Quote: ... and also on a Qubit 2.0 fluorometer (Life Technologies), to confirm accurate RNA abundance (data not shown) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were analyzed using a 4-12 % Bis-Tris NuPAGE SDS-PAGE gel (Life Technologies) and separated by molecular weight using denaturing electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... The NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, Catalogue Number 78833) was employed for this procedure.
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bioRxiv - Molecular Biology 2024Quote: ... using the Bolt Mini Blot Module (Life Technologies) and western blot carried out using the iBind Western Blot Apparatus (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from HeLa cells using QIAzol Reagent (15,596-018, Ambion life technologies) according to the manufactures protocol followed by DNase I treatment ...
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bioRxiv - Molecular Biology 2024Quote: siRNAs against JAK1 (UP and DOWN) were purchased from Life Technologies catalog number AM16708 and 4390824 ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrations were measured on a Nanodrop One (ThermoFisher). A total of 1 μg of RNA was used as input for cDNA synthesis reactions by using the Omniscript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined using a Qubit™ protein assay kit (Life Technologies) and approximately 250 ng of each protein loaded per lane ...
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bioRxiv - Molecular Biology 2024Quote: ... Data were acquired by using Xcalibur (version 2.8, Thermo Scientific Inc.). MS data were collected by top-15 data-dependent acquisition ...
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bioRxiv - Molecular Biology 2024Quote: ... The resultant RNA (40Lng) was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using custom gene-specific reverse primers (Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... and western blot carried out using the iBind Western Blot Apparatus (Life Technologies). Affinity-purified rabbit polyclonal antibodies specific to each eIF4E family member were used as the primary probe in western blotting ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 mg of nuclear extracts were bound to 50 μL of magnetic HisPur Ni-NTA beads (ThermoFisher Scientific) for 1 hr at RT on a rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted using TRIzol (Invitrogen) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were counterstained with Hoechst 34580 (H21486; ThermoFisher Scientific). Coverslips were mounted onto microscopy slides (Globe Scientific INC ...
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bioRxiv - Cancer Biology 2024Quote: ... The EdU incorporation was determined with the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay kit (C10424; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody incubation (Alexa Fluor 594 anti-rabbit, Invitrogen #A11037, 1:500) was done in 2% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides generated were analyzed by electrospray ionization on an Elite tandem orbitrap mass spectrometer (Thermo Scientific Inc). Nanoflow HPLC was performed by using a Waters NanoAcquity HPLC system (Waters Corporation) ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems) and custom primers (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The HPLC system was coupled to Orbitrap Fusion mass spectrometer (Thermo Scientific) through a Nanospray Flex Ion Source ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was quantified on a Nanodrop 1000 (Thermo Fisher) and also on a Qubit 2.0 fluorometer (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were processed using the In-Gel Tryptic Digestion Kit (Thermo Scientific, Inc.) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred onto polyvinylidene difluoride membranes (Invitrogen, CA, USA). Membranes were blocked with 3% skimmed milk for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to the 96 well plate (Thermo Fisher Scientific, MA, USA) and luciferase reporter activities were measured by luminometer (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... and random hexamer primers (Thermo Scientific, #SO142). Real-time qPCR was performed using KAPA SYBR FAST mix (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... with random primers (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The forward transfection method was used to deliver plasmids by using Lipofectamine 3000 (Invitrogen, L3000001) or Lipofectamine LTX (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: RNA interference (25nM for siRAD51, 60nM for the rest siRNAs) was achieved with Lipofectamine RNAiMax (Life technologies, 13778075) in OPTI-MEM (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: Cells were maintained in high-glucose DMEM medium (Life Technologies, 31966047) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2024Quote: ... 2CmM L-glutamine (Life Technologies, 25030024) and 100 units/ml penicillin-streptomycin solution (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... and c-Myc using the CytoTune™-iPS 2.0 Sendai Reprogramming kit (Thermo Fisher Scientific, A16518) according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... and 100 units/ml penicillin-streptomycin solution (Life Technologies, 15140122) at 37C°C with 5% CO2 supplement ...
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bioRxiv - Molecular Biology 2024Quote: ... Fibroblasts were harvested using TrypLE Express (Thermo Fisher Scientific, 12605010) and passaged at a 1 to 4 split ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... The desired DNA product was purified with 6% TBE gel (Invitrogen) and subjected to massive parallel amplicon sequencing carried out by an Illumina sequencer (Sanger/Illumina 1.9) ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were counter-stained with DAPI (Thermo Fisher Scientific), Cells were imaged by confocal microscopy on a Zeiss LSM 800 (Zeiss ...
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bioRxiv - Molecular Biology 2024Quote: ... or Lipofectamine LTX (Invitrogen, 15338100). Gibson cloning was used to generate INTS6-GFP plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified using a QuBitTM 2.0 (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... in OPTI-MEM (Gibco, 11058021) by using reverse transfection method ...