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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Plant Biology 2020Quote: ... two complementary oligonucleotides with 4-bp overhangs (Supplemental Table 1) were annealed and inserted by a Golden Gate reaction with BpiI (Thermo Scientific) and T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated over night at 4°C with primary antibody mouse IgG2a anti-V5 tag monoclonal antibody (1/1000; Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were washed once in 1 x PBS and placed into Opti-MEM reduced serum media and into 4 mm electroporation cuvettes (Thermo Fisher) with 5ug mRNA/1 million NK cells ...
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bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibody staining was performed for overnight at 4°C on the shaker and secondary donkey anti-goat 568(1:400, Molecular Probes) was added following four washes of 0.3% PTX ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were incubated at 4°C overnight with goat anti-mouse AlexaFluor 488 nm (1:500) and anti-rabbit AlexaFluor 647 nm (Molecular Probes) in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... was carried out overnight at 4° C followed by secondary antibodies (conjugated with Alexa Fluor 488 or Alexa Fluor 647 (1:250; Molecular Probes, Invitrogen) at RT for 4-5 hours ...
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bioRxiv - Immunology 2019Quote: ... All elution fractions were evaluated for their purity using 12% SDS–PAGE and 11 purified 1 ml IgG fractions were combined and dialyzed overnight at 4°C against 4L of PBS (pH 7.4) using SnakeSkin dialysis tubing with 1 kDa cutoff (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039 ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted supernatants were pre-cleared for 1 hr at 4 °C on a rotator with 50 μL of Protein-A Dynabeads (Life Technologies) pre-blocked overnight in ChIP buffer + 0.01% SDS and 100 μg ultrapure BSA (Ambion) ...
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bioRxiv - Molecular Biology 2021Quote: The cDNA reactions were diluted 1:50 and 4 µL was used per 10 µL qRT-PCR reaction with PowerUp SYBR Green Master Mix (ThermoFisher, A25742) and final primer concentrations of 500 nM in 384-well plates (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in blocking buffer with secondary antibodies at 4°C on an orbital shaker for three hours (dilution 1:500; Alexa Fluor Donkey anti-Goat 633 (Invitrogen A21082), Donkey anti-rabbit Cy3 (Jackson Immuno #711-165-152)) ...
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bioRxiv - Neuroscience 2019Quote: ... then the supernatant was removed and the pellet was resuspended in in label-free buffer [100 mM Tris-HCl (pH7.6) 4% (w/v) SDS] containing 1% protease cocktail inhibitor (Thermo Fisher, UK). Homogenates were centrifuged at 20,000 x g for 20 minutes at 4°C with the soluble fraction of each sample transferred to Lo-Bind tubes (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... for 1 h at 4 °C and immunoprecipitations were performed with 30 μL of Pierce Anti-HA Agarose (Thermo Fisher Scientific) per sample rotating overnight at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Biotinylated mPA was then modified with 10 equivalents of sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC; Thermo Fisher Scientific) at r.t ...
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bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... was measured by live ratio-fluoresce microscopy of ATT pre-treated cells after incubation in 80 µl/well of DPBS supplemented with 1 µM di-4-ANEPPDHQ (D36802, Thermo Fisher) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... samples were incubated for 24 h at 4°C with secondary antibody Alexa Fluor 647 anti-rabbit (1:500, 4414, Molecular Probes) diluted in IF-buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... actin cortices were staining by overnight incubation at 4°C in 1:100 rhodamine-phalloidin (stock in methanol; Fisher Scientific #10125092). For TUNEL staining using the “In Situ Cell Death Detection Kit TMR” (Roche #12156792910) ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti–mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and the expression plasmid encoding the GP gene of an EBOV Makona strain (GenBank accession no. KJ660346) or BDBV Uganda strain (GenBank accession no. KR063673) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher). After 48-72 h ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific) and mouse anti-SST antibody (1:250 ...
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bioRxiv - Biochemistry 2021Quote: The cells were plated at 4 × 104 cells well-1 in a Nunclon Delta-Treated 96-well plate (Thermo Scientific 167008). After allowing an overnight growth for cells to adhere ...
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bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000; cat# 51-6900, Invitrogen/ThermoFisher, Waltham, MA) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in serum before incubation with mouse monoclonal β-endorphin primary antibody (Abeam ab54205,1:200) for 24 h at 4°C and Alexa-fluor 488 chicken anti-mouse secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were run on a 4%–12% polyacrylamide gel for 1 hour at 150 V and colored with PageBlue Protein staining solution (Thermo Scientific) overnight.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... or 1 cm 4-12% Bis-Tris gel for ProQ diamond stain with 1x MOPS running buffer (Thermo Fisher Scientific, NP0001), transferred onto a PVDF membrane (EMD Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Microbiology 2020Quote: ... the samples were purified to obtain only the bacterial cells by first diluting them 1/4 in UltraPure™ DNase/RNase-Free distilled water (Invitrogen), then centrifuging twice at 4 226 g for 10 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... The lysate was clarified by centrifugation (18 000g, 30 min, 4°C) and applied to a 1-ml Ni-NTA column (HisPur, ThermoFisher Scientific). After incubation at 4°C for 1 h the resin was washed with 50 ml of lysis buffer and eluted with 6 ml lysis buffer plus 250 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: GTPases at a final concentration of 10 μM were loaded with sub-stoichiometric (1/4 [GTPase]) concentrations of GDP-BODIPY-FL (Thermo Scientific) in the presence of 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were fixed in ice-cold 100% ethanol for 1 minute followed by 4 minutes of staining with 0.0001% Cresyl Violet (ACROS Organics, AC229630050). Slides were dehydrated using 95% and 100% ethanol followed by xylene for 1 minute each ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cells were centrifuged at 14,000 rpm for 1 h at 4°C to remove the cell debris and the supernatant was incubated to Ni2+-NTA resin (Thermo Fisher) pre equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... overnight at 4 °C and visualised with a fluorescent secondary antibody (goat-anti-mouse IgG Alexa 488-conjugated (1:5000, A11029, Life Technologies)) ...
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bioRxiv - Microbiology 2020Quote: ... Differentiated THP-1 cells are adherent and were seeded in 4-well Lab-TekII Chamber Slides (Nalgene Nunc International, Rochester, NY) at a density of 3 × 105 cells per well ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ml of culture was transferred to a foil wrapped tube and incubated with FM 4-64 (1 µM in DMSO, Molecular Probes) for 30 min at 30°C ...
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bioRxiv - Bioengineering 2022Quote: DNA transfections were performed by seeding HEK293T cells in 12 well plates at 25% confluency and adding 1 μg of each DNA construct and 4 μL of Lipofectamine 2000 (Thermo Fisher). RNA transfections were performed by adding 1 μg of each RNA construct and 3.5 μL of Lipofectamine MessengerMax (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... after which gels were transferred onto Amersham Protran 0.2 µm nitrocellulose membrane (Dutscher, 10600001) immersed at 4°C in NuPAGE Transfer Buffer (ThermoFisher, NP0006-1) for 90 minutes at 60V ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were separated from the lysate and washed four times with 1 ml of CoIP buffer before resuspending in 15 μl of 1 x loading buffer (NuPAGE LDS sample buffer (4 x) and NuPAGE sample reducing agent (10 x) (ThermoFisher Scientific) diluted to 1 x with H2O) ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation of sections from cerebral organoids in primary antibodies solution was performed overnight at 4°C and in secondary antibodies solution at room temperature for 1 hour (Alexa Fluor, Molecular Probes). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2022Quote: ... which were subsequently vitrified at 4°C and 100% humidity for 4.5 s with a blotting force of -1 on a Vitrobot IV (Thermo Fisher Scientific) with standard Vitrobot filter paper (ø55/20 mm ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... This was followed by blocking and incubation with appropriate primary antibodies overnight at 4 °C and species specific secondary antibodies [polyclonal Alexa fluor 488 goat anti-rabbit,1:1000 (A11070 of Invitrogen Biosciences), polyclonal Alexa fluor 555 rabbit anti-mouse,1:1000 (A21427 of Invitrogen Biosciences)] for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating slices were then incubated 48h at 4° with a mix of anti-GFP (1:5000, Ref. A10262, Invitrogen, USA) and anti-NeuN (1:1000 ...